Dosage Des Lipides Des Fèces. Extraction Séparée, Importance Et Composition en Acides Gras Des Lipides Non Saponifiés Et De Ceux Des Complexes Insolubles

Four feeds, two duodenal digesta samples, two ileal digesta samples and two faecal samples were analysed in quintuplicate for their long-chain fatty acid content by a new method (direct methylation) whereby the dried samples were heated with benzene and 5 % w/v methanolic HCl in screw-capped bottles at 70°C for 2 h. The methyl esters, were extracted with hexane, purified by thin-layer chromatography, and measured by gas-liquid chromatography. The values obtained were compared with those obtained using an established method (saponification) in which the dried samples were refluxed with ethanolic KOH, the fatty acids liberated by acidification, extracted and methylated by refluxing with 5 % wlv methanolic HCl and the methyl esters extracted, purified and measured as before. Statistical analysis indicated no particular bias for either method though in general the direct methylation appeared to have a lower variance than saponification. A regression analysis of the direct methylation means (2) against the saponification means (i) gave the equation: -d=0.0073 + 0.9883 r2 = 0.994 (n=96) (i 0.0255) (i 0.0079)

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Direct methylation of long‐chain fatty acids in feeds, digesta and faeces without prior extraction

Outen

Beever

Fenlon

1976

J Sci Food Agric

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Influence of the type of fat in milk replacers on hydrolysis and digestion by the pre‐ruminant calf

Johnson

Leibholz

1980

J Sci Food Agric

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Pre-ruminant Friesian calves were fed milk replacers containing three different fat sources to observe their effect on abomasal outflow, triglyceride hydrolysis and nutritive value. The calves were fitted with re-entrant cannulae in the duodenum and ileum. The milk replacers were prepared by homogenising 30% butterfat, tallow or soya-bean oil with skim milk prior to spray-drying. These milk replacers (500 g), and also skim milk, were fed once daily dissolved in 3500 ml of water. Total collections of abomasal and ileal outflow were made for 24 h periods. There were no appreciable differences in abomasal emptying of fresh matter, dry matter, total fat, or in the pH of the digesta when calves received milk replacers containing butterfat or tallow, but some inhibition of gastric emptying occurred when skim milk was fed. The extent of hydrolysis of the fats which occurred in the abomasum, as evidenced from free fatty acid concentrations in the abomasal fat, decreased in the order: butterfat > soya-bean >tallow. There was an inverse relationship between the chain length of the fatty acids and their rate of hydrolysis. Extensive biohydrogenation in the large intestine appeared to occur only when the highly unsaturated soya-bean oil was fed. The mean apparent digestibilities of the total dietary fats were: butterfat, 95.0%; tallow, 55.3 "/, and soya-bean oil, 93.9%. Between 4-16 g more fat than was ingested flowed from the abomasum during the period 0-24 h after calves received skim milk, which represented 1 4 % of the total abomasal outflow. Metabolic faecal fat excretion was 5.93 k2.02 g 100 g-l faecal dry matter.

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Effect of addition of tallow and lecithin in the diet of weanling piglets on the apparent total tract and ileal digestibility of fat and fatty acids

Souza

Peiniau

Mounier

et al. 1995

Animal Feed Science and Technology

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Dosage Des Lipides Des Fèces. Extraction Séparée, Importance Et Composition en Acides Gras Des Lipides Non Saponifiés Et De Ceux Des Complexes Insolubles

Cited by 10 publications

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Direct methylation of long‐chain fatty acids in feeds, digesta and faeces without prior extraction

Direct methylation of long‐chain fatty acids in feeds, digesta and faeces without prior extraction

Influence of the type of fat in milk replacers on hydrolysis and digestion by the pre‐ruminant calf

Effect of addition of tallow and lecithin in the diet of weanling piglets on the apparent total tract and ileal digestibility of fat and fatty acids

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