Dorsoventral Differences in Intrinsic Properties in Developing CA1 Pyramidal Cells

Marcelin, Béatrice; Liu, Zhiqiang; Chen, Yuncai; Lewis, Alan S.; Becker, Albert; McClelland, Shawn; Chetkovich, Dane M.; Migliore, Michele; Baram, Tallie Z.; Esclapez, Monique; Bernard, Christophe

doi:10.1523/jneurosci.5870-11.2012

Cited by 40 publications

(56 citation statements)

References 52 publications

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“…Indeed, in rats, membrane conductances near rest differ from DH to VH (i.e. Kv723, HNCN24 and GIRK25) and dendritic surface area of DH neurons is larger than that of VH ones101126. We did not observe any significant difference in the average membrane capacitance between VH and DH cells but we measured a similar specific conductance, suggesting that differences in RMP and Rin might depend on both cell dimension and ionic conductances.…”

Section: Discussionmentioning

confidence: 46%

Electrophysiological Properties of CA1 Pyramidal Neurons along the Longitudinal Axis of the Mouse Hippocampus

Milior

Castro

Sciarria

et al. 2016

Sci Rep

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Evidence for different physiological properties along the hippocampal longitudinal axis is emerging. Here, we examined the electrophysiological features of neurons at different dorso-ventral sites of the mouse CA1 hippocampal region. Cell position was defined with respect to longitudinal coordinates of each slice. We measured variations in neuronal excitability, subthreshold membrane properties and neurotransmitter responses along the longitudinal axis. We found that (i) pyramidal cells of the dorsal hippocampus (DH) were less excitable than those of the ventral hippocampus (VH). Resting Membrane Potential (RMP) was more hyperpolarized and somatic Input Resistance (Ri) was lower in DH compared to VH. (ii) The Paired-pulse ratio (PPR) of focally induced synaptic responses was systematically reduced from the DH to the VH; (iii) Long-term-potentiation was most pronounced in the DH and fell gradually in the intermediate hippocampus and in the VH; (iv) the frequency of miniature GABAergic events was higher in the VH than in the DH; (v) the PPR of evoked inhibitory post-synaptic current (IPSC) was higher in the DH than in the VH. These findings indicate an increased probability of both GABA and glutamate release and a reduced plasticity in the ventral compared to more dorsal regions of the hippocampus.

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Section: Discussionmentioning

confidence: 46%

Electrophysiological Properties of CA1 Pyramidal Neurons along the Longitudinal Axis of the Mouse Hippocampus

Milior

Castro

Sciarria

et al. 2016

Sci Rep

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“…Surprisingly, the dorso-ventral gradient of resonance and I h properties of CA1 pyramidal cell dendrites are reversed as compared with those found at earlier stages of development (13). The meaning and mechanisms for such changes remain to be determined.…”

Section: Discussionmentioning

confidence: 82%

“…In Vitro Electrophysiology-Hippocampal 350-m-thick dorsal (coronal) or ventral (sagittal with a 45°angle) slices were prepared from male adult Wistar Han rats, as described (13). Slices were then transferred to a holding chamber at room temperature in normal artificial cerebrospinal fluid.…”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, the resonance (or band pass filtering) properties in the soma of these cells also change along this axis, together with the properties of the hyperpolarization-activated I h current (8 -12). Similar principles have been described in CA1 pyramidal cells during development, but whether they persist in adult animals is not known development (13). The size of the place fields coded by CA1 pyramidal cells increases along the dorso-ventral axis (14,15).…”

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confidence: 85%

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Differential Dorso-ventral Distributions of Kv4.2 and HCN Proteins Confer Distinct Integrative Properties to Hippocampal CA1 Pyramidal Cell Distal Dendrites

Marcelin

Lugo

Brewster

et al. 2012

Journal of Biological Chemistry

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Background:Information is differentially processed in the dorsal versus ventral hippocampus. Results: Expression levels of Kv4.2 and HCN1 varied and conferred distinct integrative properties to CA1 pyramidal cell dendrites in dorsal and ventral hippocampus. Conclusion: Molecular and physiological differences provide the basis for the specific properties of dorsal and ventral CA1 pyramidal cells. Significance: Channel expression and function enable specific processing roles.

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“…More generally, while most studies to date focused on the dorsal hippocampus, substantial cognitive (e.g. fear vs. declarative learning), connectivity (Ropireddy and Ascoli, 2011; Cardoso-Cruz et al, 2014; Krook-Magnuson et al, 2012; Lee et al, 2014), and biophysical (Graves et al, 2012; Malik et al, 2016; Marcelin et al, 2012b; Marcelin et al, 2012a) differences exist in the septotemporal directions towards the posterior and ventral regions. Given the explicit anatomical mapping of the ISH intensity and density values across the rostro-caudal extent in the ABA, the present study could in principle be expanded in the future to account for these gradients, possibly refining the neuron type classification of Hippocampome.org.…”

Section: 0 Discussionmentioning

confidence: 99%

Molecular fingerprinting of principal neurons in the rodent hippocampus: A neuroinformatics approach

Hamilton

White

Rees

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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Neurons are often classified by their morphological and molecular properties. The online knowledge base Hippocampome.org primarily defines neuron types from the rodent hippocampal formation based on their main neurotransmitter (glutamate or GABA) and the spatial distributions of their axons and dendrites. For each neuron type, this open-access resource reports any and all published information regarding the presence or absence of known molecular markers, including calcium-binding proteins, neuropeptides, receptors, channels, transcription factors, and other molecules of biomedical relevance. The resulting chemical profile is relatively sparse: even for the best studied neuron types, the expression or lack thereof of fewer than 70 molecules has been firmly established to date. The mouse genome-wide in situ hybridization mapping of the Allen Brain Atlas provides a wealth of data that, when appropriately analyzed, can substantially augment the molecular marker knowledge in Hippocampome.org. Here we focus on the principal cell layers of dentate gyrus (DG), CA3, CA2, and CA1, which together contain approximately 90% of hippocampal neurons. These four anatomical parcels are densely packed with somata of mostly excitatory projection neurons. Thus, gene expression data for those layers can be justifiably linked to the respective principal neuron types: granule cells in DG and pyramidal cells in CA3, CA2, and CA1. In order to enable consistent interpretation across genes and regions, we screened the whole-genome dataset against known molecular markers of those neuron types. The resulting threshold values allow over 6000 very-high confidence (>99.5%) expressed/not-expressed assignments, expanding the biochemical information content of Hippocampome.org more than five-fold. Many of these newly identified molecular markers are potential pharmacological targets for major neurological and psychiatric conditions. Furthermore, our approach yields reasonable expression/non-expression estimates for every single gene in each of these four neuron types with >90% average confidence, providing a considerably complete genetic characterization of hippocampal principal neurons.

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Dorsoventral Differences in Intrinsic Properties in Developing CA1 Pyramidal Cells

Cited by 40 publications

References 52 publications

Electrophysiological Properties of CA1 Pyramidal Neurons along the Longitudinal Axis of the Mouse Hippocampus

Electrophysiological Properties of CA1 Pyramidal Neurons along the Longitudinal Axis of the Mouse Hippocampus

Differential Dorso-ventral Distributions of Kv4.2 and HCN Proteins Confer Distinct Integrative Properties to Hippocampal CA1 Pyramidal Cell Distal Dendrites

Molecular fingerprinting of principal neurons in the rodent hippocampus: A neuroinformatics approach

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