Dormant and non-dormant Castanea sativa Mill. buds require different polyvinylpyrrolidone concentrations for optimal RNA isolation

Santamaría, Mª. Estrella; Toorop, Peter E.; Rodrı́guez, Roberto; Cañal, Ma Jesús

doi:10.1016/j.plantsci.2009.10.002

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…The samples were freeze-dried and finely ground with a pestle and mortar. Plant extracts were prepared according to Kirca and Arslan (2008) with modifications regarding the amounts of plant materials (Santamaría et al 2010). The dried plant material (0.075 g) was weighed in a centrifuge tube and 1.5 ml of methanol was added.…”

Section: Measurement Of Total Phenolic Contentmentioning

confidence: 99%

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Quiala

Cañal

Meijón

et al. 2011

Plant Cell Tiss Organ Cult

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In vitro shoot proliferation of Tectona grandis L. (teak) using temporary immersion system (TIS) and different concentrations of BA is presented. Shoots grown on semi-solid MS medium supplemented with 2% (w/v) sucrose and 4.44 µM of BA were used as starting materials. In the TIS, three BA concentrations (2.22, 4.44 and 6.66 µM) and a cytokinin-free medium (CK-free medium) were assayed. A high average number of shoot was reached, applying 4.44 and 6.66 µM of BA (7.7 and 10.3 shoots/explant), respectively. The high BA concentrations decreased the accumulation of phenolic compounds and the deposition of lignin in the vascular cells of the teak shoots. The morphometric analysis by scanning microscopy revealed that the leaves of the shoots cultured in the TIS in Ck-free medium and with 2.22 and 4.44 µM of BA showed elliptical stomata; however, in the leaves developed with 6.66 µM of BA, the stomata were majorly ring-shaped, raised and wide open. Deformed stomata with broken epidermis of the guard cells, typical of hyperhydric leaves, were also observed. The survival percentages after ex vitro rooting on the IBA (492.1 µM) solution were higher in plantlets from CK-free medium and with 2.22 µM of BA, (96.7 and 91.7%, respectively) than those cultured on semi-solid medium (73%). The shoots from both TIS treatments developed a good root system. Plantlets from 6.66 µM of BA showed the lowest survival percentages (60%). A survival percentage was 100% two weeks after transplanting, and three months after ex vitro transfer, the plants were ready for field plantation. Here we present the first report of the successful propagation of teak by TIS.

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Section: Measurement Of Total Phenolic Contentmentioning

confidence: 99%

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Quiala

Cañal

Meijón

et al. 2011

Plant Cell Tiss Organ Cult

Self Cite

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“…3 mg mL 21 for ND buds and 14 . 3 mg mL 21 for D buds (Santamaría et al, 2010). The RNA was used to generate two subtraction libraries, one enriched with dormancy-associated gene transcripts (D library) and the other enriched with gene transcripts associated with the absence of dormancy (ND library), using the Clontech PCR-Select TM cDNA Subtraction Kit (Clontech, Saint-Germain-en-Laye, France).…”

Section: Suppression Subtractive Hybridizationmentioning

confidence: 99%

Transcriptome analysis of chestnut (Castanea sativa) tree buds suggests a putative role for epigenetic control of bud dormancy

et al. 2011

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“…Total RNA from seedlings, 3-weel-old-rosettes and roots was extracted by the phenol/chloroform method, followed by precipitation with 8 M LiCl according to Oñate-Sánchez and Vicente-Carbajosa (2008). RNA form mature seeds was isolated by the hot borate method described by Santamaria et al (2010). Regarding N. benthamiana assays, total RNA was extracted from plants agroinfiltrated with the control vector pmdc107ø, the mGFP::BP::mCherryFP and the mCherryFP::BP::mGFP constructs, by the TRIZOL reagent following manufacturer instructions (Ambion Austin TX).…”

Section: Methodsmentioning

confidence: 99%

Plant Defenses Against Pests Driven by a Bidirectional Promoter

et al. 2019

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The plant defense responses to pests results in the synchronized change of a complex network of interconnected genes and signaling pathways. An essential part of this process is mediated by the binding of transcription factors to the specific responsive cis -elements within in the promoters of phytophagous-responsive genes. In this work, it is reported the identification and characterization of a bidirectional promoter that simultaneously co-regulate two divergent genes, At5g10300 and At5g10290 , upon arthropod feeding. Computational analysis identified the presence of cis -elements within the intergenic region between two loci, mainly from the DOF but also from the AP2/ERF, Golden 2-like and bHLH families. The function of the bidirectional promoter was analyzed using two enhanced variants of the GFP and CherryFP reporter genes, in both orientations, in transient tobacco and stably transformed Arabidopsis plants. Promoter activity was tested in response to feeding of Tetranychus urticae and Pieris brassicae , as well as wounding, flagellin and chitin treatments. Using RT-qPCR assays and confocal microscopy, it was shown that all treatments resulted in the induction of both reporter genes. Furthermore, our findings revealed the asymmetric character of the promoter with stronger activity in the forward than in the reverse orientation. This study provides an example of a bidirectional promoter with a strong potential to be used in plant biotechnology in pest control that requires stacking of the defense genes.

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Dormant and non-dormant Castanea sativa Mill. buds require different polyvinylpyrrolidone concentrations for optimal RNA isolation

Cited by 7 publications

References 33 publications

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

Transcriptome analysis of chestnut (Castanea sativa) tree buds suggests a putative role for epigenetic control of bud dormancy

Plant Defenses Against Pests Driven by a Bidirectional Promoter

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