Dopamine synthesis by non-dopaminergic neurons expressing individual complementary enzymes of the dopamine synthetic pathway in the arcuate nucleus of fetal rats

Ugrumov, M. V.; Melnikova, V. I.; Lavrentyeva, A. V.; Кудрин, В. С.; Rayevsky, K.S.

doi:10.1016/j.neuroscience.2004.01.002

Cited by 54 publications

(39 citation statements)

References 30 publications

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“…As postulated by Weihe et al (2006), cortical TH-IR (dopaergic) neurons may supply L-DOPA to neighboring AADC-containing, TH-negative neurons, which then may be capable of synthesizing dopamine. Dopamine production by monoenzymatic cells was reported in the hypothalamic arcuate nucleus of developing rats (Ugrumov et al, 2004). On the other hand, L-DOPA may function as a transmitter, as posited by Misu and colleagues (2003).…”

Section: Discussionmentioning

confidence: 87%

Neurochemical characterization of tyrosine hydroxylase-immunoreactive interneurons in the developing rat cerebral cortex

et al. 2008

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Understanding the development of cortical interneuron phenotypic diversity is critical because interneuron dysfunction has been implicated in several neurodevelopmental disorders. Here, tyrosine hydroxylase (TH)-immunoreactive neurons in the developing and adult rat cortex were characterized in light of findings regarding interneuron neurochemistry and development. Cortical THimmunoreactive neurons were first observed two weeks postnatally and peaked in number three weeks after birth. At subsequent ages, the number of these cell profiles was gradually reduced, and they were seen less frequently in adults. No DNA fragmentation or active caspase 3 was observed in cortical TH cells at any age examined, eliminating cell death as an explanation for the decrease in cell number. Although cortical TH cells reportedly fail to produce subsequent catecholaminergic enzymes, we found that the majority of these cells at all ages contained phosphorylated TH, suggesting that the enzyme may be active and producing L-DOPA as an end-product. Morphological criteria and colocalization of some TH cells with glutamic acid decarboxylase suggests that these cells are interneurons. Previously, parvalbumin, somatostatin, and calretinin were demonstrated in nonoverlapping subsets of interneurons. Cortical TH neurons colocalized with calretinin but not with parvalbumin or somatostatin. These findings suggest that the transitory increase in TH cell number is not due to cell death but possibly due to alterations in the amount of detectable TH present in these cells, and that at least some cortical TH-producing interneurons belong to the calretinin-containing subset of interneurons that originate developmentally in the caudal ganglionic eminence.

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Section: Discussionmentioning

confidence: 87%

Neurochemical characterization of tyrosine hydroxylase-immunoreactive interneurons in the developing rat cerebral cortex

et al. 2008

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“…S5 and Movies S1-S48 (AVI)]. These neurons may produce levodopa, similar to more rostral neurons that are reportedly involved in interneuronal shuttling for catecholamine production (28). Also, some enkephalin (Penk1) expression was lost from the PB and DN, serotonin receptor 1a (Htr1a) disappeared from the Dll, and somatostatin receptor 2 (Sstr2) was lost from the EGL and DC [Movies S1-S48 (AVI)].…”

Section: Novel Math1mentioning

confidence: 82%

Excitatory neurons of the proprioceptive, interoceptive, and arousal hindbrain networks share a developmental requirement for Math1

Rose

Ahmad²,

Thaller³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

123

208

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Hindbrain networks important for sensation and arousal contain diverse neuronal populations with distinct projections, yet share specific characteristics such as neurotransmitter expression. The relationship between the function of these neurons, their developmental origin, and the timing of their migration remains unclear. Mice lacking the proneural transcription factor Math1 (Atoh1) lose neurons essential for hearing, balance, and unconscious proprioception. By using a new, inducible Math1 Cre*PR allele, we found that Math1 is also required for the conscious proprioceptive system, including excitatory projection neurons of the dorsal column nuclei and for vital components of the interoceptive system, such as Barrington's nucleus, that is closely associated with arousal. In addition to specific networks, Math1 lineages shared specific neurotransmitter expression, including glutamate, acetylcholine, somatostatin, corticotropin releasing hormone, and nitric oxide. These findings identify twenty novel Math1 lineages and indicate that the Math1 network functions partly as an interface for conscious (early-born) and unconscious (late-born) proprioceptive inputs to the cortex and cerebellum, respectively. In addition, these data provide previously unsuspected genetic and developmental links between proprioception, interoception, hearing, and arousal.auditory ͉ dorsal columns ͉ medial lemniscus ͉ proneural ͉ rhombic lip

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“…Misu and colleagues have suggested, based on expression of TH, but not AADC, the existence in brain cardiovascular regulatory nuclei of neurons that elaborate dopa, but not dopamine, and hypothesized that these areas, including the nucleus tractus solitarius (NTS) and area postrema (AP), might function in interneuronal "dopaergic" signaling (Misu et al, 2002;Misu et al, 2003). Ugrumov and colleagues have identified in developing rodent hypothalamus, TH-positive, AADC-negative "monoenzymatic" neurons that function to supply L-dopa to TH-negative, AADC-positive cells capable of synthesizing dopamine from L-dopa, and apparently, of packaging it for exocytotic, regulated secretion (Balan et al, 2000;Ugrumov et al, 2002;Ugrumov et al, 2004). Mapping of monoamine plasma membrane and vesicular transporters in rodents during development has identified VMAT2- Schütz et al, 1998a,b) and plasma membrane serotonin transporter (SERT)-expressing thalamocortical projection neurons (Lebrand et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Despite reports in the literature indicating that TH-positive, AADC-negative neurons exist in CNS as do TH-negative, AADC-positive (nonserotonergic, nonhistaminergic) neurons (Komori et al, 1991;Kitahama et al, 1996;Ugrumov, 1997;Ikemoto et al, 1998a,b;Kitahama et al, 1998;Balan et al, 2000;Ershov et al, 2002a,b;Misu et al, 2002;Ugrumov et al, 2002;Misu et al, 2003;Ugrumov et al, 2004;Ershov et al, 2005), their functional status has not been fully assessed because the presence or absence of VMAT2 in these neurons was unknown.…”

Section: Introductionmentioning

confidence: 99%

Three Types of Tyrosine Hydroxylase-Positive CNS Neurons Distinguished by Dopa Decarboxylase and VMAT2 Co-Expression

et al. 2006

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SUMMARY1. We investigate here for the first time in primate brain the combinatorial expression of the three major functionally relevant proteins for catecholaminergic neurotrans-mission tyrosine hydroxylase (TH), aromatic acid acid decarboxylase (AADC), and the brain-specific isoform of the vesicular monoamine transporter, VMAT2, using highly specific antibodies and immunofluorescence with confocal microscopy to visualize combinatorial expression of these proteins.2. In addition to classical TH, AADC, and VMAT2-copositive catecholaminergic neurons, two unique kinds of TH-positive neurons were identified based on co-expression of AADC and VMAT2.3. TH and AADC co-positive, but VMAT2-negative neurons, are termed "nonexocytotic catecholaminergic TH neurons." These were found in striatum, olfactory bulb, cerebral cortex, area postrema, nucleus tractus solitarius, and in the dorsal motor nucleus of the vagus.4. TH-positive neurons expressing neither AADC nor VMAT2 are termed "dopaergic TH neurons." We identified these neurons in supraoptic, paraventricular and periventricular hypothalamic nuclei, thalamic paraventicular nucleus, habenula, parabrachial nucleus, cerebral cortex and spinal cord. We were unable to identify any dopaergic (TH-positive, AADC-negative) neurons that expressed VMAT2, suggesting that regulatory mechanisms exist for shutting off VMAT2 expression in neurons that fail to biosynthesize its substrates.5. In several cases, the corresponding TH phenotypes were identified in the adult rat, suggesting that this rodent is an appropriate experimental model for further investigation of these TH-positive

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Dopamine synthesis by non-dopaminergic neurons expressing individual complementary enzymes of the dopamine synthetic pathway in the arcuate nucleus of fetal rats

Cited by 54 publications

References 30 publications

Neurochemical characterization of tyrosine hydroxylase-immunoreactive interneurons in the developing rat cerebral cortex

Neurochemical characterization of tyrosine hydroxylase-immunoreactive interneurons in the developing rat cerebral cortex

Excitatory neurons of the proprioceptive, interoceptive, and arousal hindbrain networks share a developmental requirement for Math1

Three Types of Tyrosine Hydroxylase-Positive CNS Neurons Distinguished by Dopa Decarboxylase and VMAT2 Co-Expression

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