Dopamine sensing with fluorescence strategy based on low temperature co-fired ceramic technology modified with conducting polymers

Baluta, Sylwia; Malecha, Karol; Zając, Dorota; Sołoducho, Jadwiga; Cabaj, Joanna

doi:10.1016/j.snb.2017.06.073

Cited by 35 publications

(19 citation statements)

References 37 publications

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“…They create an appropriate microenvironment to immobilize the protein and act as a transducer during the transfer of electric charge. 9,21 In fact, CP is easy to manipulation of their electrical and physicochemical characteristics through reduction or oxidation process (n-type or p-type materials). 22 Furthermore, conductive polymers have a hydrophobic backbone that facilitates stackable p-p molecules, making the interaction between the protein and the polymer matrix stronger.…”

Section: Synthesis and Characterization Of Monomer 47-bis(5-(34ethymentioning

confidence: 99%

“…Due to their high durability and stability, they are a suitable material for the construction of sensor devices. 8,9 The performance of the biosensor depends mainly on the structure of the surface, the interaction between the enzyme and the surface of the electrode and the protection of the three-dimensional structure of the protein. Therefore conductive polymers have proven to be one of the most useful transducers due to their simplicity of production.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical biosensor for detection of 17β-estradiol using semi-conducting polymer and horseradish peroxidase

et al. 2020

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A convenient electrochemical sensing pathway for 17b-estradiol detection was investigated. The system is based on a conducting polymer and horseradish peroxidase (HRP) modified platinum (Pt) electrode. The miniature estradiol biosensor was developed and constructed through the immobilization of HRP in an electroactive surface of the electrode covered with electroconducting polymerpoly(4,7-bis(5-(3,4ethylenedioxythiophene)thiophen-2-yl)benzothiadiazole). The detection strategy is based on the fact that 17b-estradiol (E2) and pyrocatechol (H 2 Q) are co-substrates for the HRP enzyme. HRP, which does not react with E2, in the presence of H 2 O 2 catalyses the oxidation of H 2 Q to o-benzoquinone (Q). With the optimized conditions, such constructed biosensing system demonstrated a convenient level of sensitivity, selectivity in a broad linear range -0.1 to 200 mM with a detection limit of 105 nM. Furthermore, the method was successfully applied for hormone detection in the presence of potential interfering compounds (ascorbic acid, estriol, estrone, uric acid and cholesterol). Fig. 2 Schematic representation of the 17b-estradiol enzyme-based biosensor.9080 | RSC Adv., 2020, 10, 9079-9087This journal is

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Section: Synthesis and Characterization Of Monomer 47-bis(5-(34ethymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensor for detection of 17β-estradiol using semi-conducting polymer and horseradish peroxidase

et al. 2020

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“…Their energy gaps, ionization potentials, and electron affinity are in semiconductor-specific ranges. From our previous study, the results clearly show that it is not necessary to use doping in compounds obtained in our laboratory to ensure the transport of electrons between the active center of the protein and the electrode [44,45]. This fact forms the basis of our research.…”

Section: Characterization Of Polymersmentioning

confidence: 77%

Enzymatic Platforms for Sensitive Neurotransmitter Detection

Baluta

Zając

Szyszka

et al. 2020

Sensors

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A convenient electrochemical sensing pathway was investigated for neurotransmitter detection based on newly synthesized silole derivatives and laccase/horseradish-peroxidase-modified platinum (Pt)/gold (Au) electrodes. The miniature neurotransmitter’s biosensors were designed and constructed via the immobilization of laccase in an electroactive layer of the Pt electrode coated with poly(2,6-bis(3,4-ethylenedioxythiophene)-4-methyl-4-octyl-dithienosilole) and laccase for serotonin (5-HT) detection, and a Au electrode modified with the electroconducting polymer poly(2,6-bis(selenophen-2-yl)-4-methyl-4-octyl-dithienosilole), along with horseradish peroxidase (HRP), for dopamine (DA) monitoring. These sensing arrangements utilized the catalytic oxidation of neurotransmitters to reactive quinone derivatives (the oxidation process was provided in the enzymes’ presence). Under the optimized conditions, the analytical performance demonstrated a convenient degree of sensitivity: 0.0369 and 0.0256 μA mM−1 cm−2, selectivity in a broad linear range (0.1–200) × 10−6 M) with detection limits of ≈48 and ≈73 nM (for the serotonin and dopamine biosensors, respectively). Moreover, the method was successfully applied for neurotransmitter determination in the presence of interfering compounds (ascorbic acid, L-cysteine, and uric acid).

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“…Previous research connected with dopamine detection using the laccase biosensor showed a promising method based on indirect strategy. It was mainly related to graphene quantum dots (GQDs) and it could be used thanks to the ability of dopamine to spontaneous polymerization in alkaline environments [40]. Consequently, despite the lack of adequate literature data on spontaneous epinephrine polymerization, it was decided to investigate whether such phenomenon was occurring and whether sensors based on quantum dots could also be used in the detection of EP.…”

Section: Detection Principles and Optical Ep Determinationmentioning

confidence: 99%

Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics

Baluta

Malecha

Świst

et al. 2020

Sensors

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A novel fluorescence-sensing pathway for epinephrine (EP) detection was investigated. The ceramic-based miniature biosensor was developed through the immobilization of an enzyme (laccase, tyrosinase) on a polymer—poly-(2,6-di([2,2′-bithiophen]-5-yl)-4-(5-hexylthiophen-2-yl)pyridine), based on low temperature cofired ceramics technology (LTCC). The detection procedure was based on the oxidation of the substrate, i.e., in the presence of the enzyme. An alternative enzyme-free system utilized the formation of a colorful complex between Fe2+ ions and epinephrine molecules. With the optimized conditions, the analytical performance illustrated high sensitivity and selectivity in a broad linear range with a detection limit of 0.14–2.10 nM. Moreover, the strategy was successfully used for an EP injection test with labeled pharmacological samples.

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Dopamine sensing with fluorescence strategy based on low temperature co-fired ceramic technology modified with conducting polymers

Cited by 35 publications

References 37 publications

Electrochemical biosensor for detection of 17β-estradiol using semi-conducting polymer and horseradish peroxidase

Electrochemical biosensor for detection of 17β-estradiol using semi-conducting polymer and horseradish peroxidase

Enzymatic Platforms for Sensitive Neurotransmitter Detection

Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics

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