Dopamine-Modified Hyaluronic Acid Hydrogel Adhesives with Fast-Forming and High Tissue Adhesion

Abstract

Commercial or clinical tissue adhesives are currently limited due to their weak bonding strength on wet biological tissue surface, low biological compatibility, and slow adhesion formation. Although catechol-modified hyaluronic acid (HA) adhesives are developed, they suffer from limitations: insufficient adhesiveness and overfast degradation, attributed to low substitution of catechol groups. In this study, we demonstrate a simple and efficient strategy to prepare mussel-inspired HA hydrogel adhesives with improved degree of substitution of catechol groups. Because of the significantly increased grafting ratio of catechol groups, dopamine-conjugated dialdehyde–HA (DAHA) hydrogels exhibit excellent tissue adhesion performance (i.e., adhesive strength of 90.0 ± 6.7 kPa), which are significantly higher than those found in dopamine-conjugated HA hydrogels (∼10 kPa), photo-cross-linkable HA hydrogels (∼13 kPa), or commercially available fibrin glues (2–40 kPa). At the same time, their maximum adhesion energy is 384.6 ± 26.0 J m–2, which also is 40–400-fold, 2–40-fold, and ∼8-fold higher than those of the mussel-based adhesive, cyanoacrylate, and fibrin glues, respectively. Moreover, the hydrogels can gel rapidly within 60 s and have a tunable degradation suitable for tissue regeneration. Together with their cytocompatibility and good cell adhesion, they are promising materials as new biological adhesives.

Multifunctional Injectable Hydrogel Dressings for Effectively Accelerating Wound Healing: Enhancing Biomineralization Strategy

“…The strong reducibility of catechol groups inspired us to introduce them to gelatin backbone to enhance the biomineralization ability of gelatin. [ 8c,9 ] To the best of our knowledge, there have been literatures that grafting dopamine (DA) on the macromolecule, such as hyaluronic acid, [ 21 ] sodium alginate, [ 22 ] and sulfobetaine methacrylate, [ 23 ] can endow them with various excellent properties. However, it has not been reported to enhance the biomineralization ability of gelatin by DA‐modification, thereby giving it a wider range of applications in wound dressings.…”

Dopamine-Grafted Hyaluronic Acid Coated Hyperbranched Poly(β-Amino Esters)/DNA Nano-Complexes for Enhanced Gene Delivery and Biosafety

“…To overcome this dilemma, we report on dopamine (DA)-grafted HA coated hyperbranched PAEs/DNA nano-complexes for enhanced gene delivery (Scheme 1). DA is a hormone and neurotransmitter in biology, while DA-grafted polymers have been found to exhibit bio-adhesive abilities, increasing cell membrane interactions [17]. Additionally, DA can be used as an active targeting ligand to enhance the internalization of nanoparticles into tumor cells with high expression of DA receptors [18].…”

Preparation and Application of Quaternized Chitosan- and AgNPs-Base Synergistic Antibacterial Hydrogel for Burn Wound Healing

“…By detecting the OD value at 450 nm, the ODex/HA-ADH and ODex/HA-ADH/HACC hydrogels had a higher cell viability (over 90%) compared with the Ag@ODex/HA-ADH/HACC hydrogel (76.41%) on day one. This may be due to the fact that dextran and hyaluronic acid were all-natural biomaterials with good biocompatibility that can promote cell proliferation [ 43 , 44 ], while AgNPs had a certain cytotoxicity [ 45 ]. Moreover, the cell viability on day seven was higher than on day one and day three, this may be attributed to the ODex/HA-ADH/HACC hydrogel promoting cell proliferation.…”