Dopamine increases the expression of tyrosine hydroxylase and aromatic amino acid decarboxylase in primary cultures of fetal neurons

Vitry, F. De; Hillion, Joëlle; Catelon, J.; Thibault, Jean; Benoliel, J.J.; Hamon, Michel

doi:10.1016/0165-3806(91)90092-w

Cited by 34 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There-fore, ligand stimulation of receptors linked to guanine nucleotide binding proteins that increase intracellular cAMP will activate TH gene transcription. Such a mechanism might explain the induction of TH expression (and, thus, a dopaminergic phenotype) in a variety of central and peripheral nervous system models (28)(29)(30)(31). Similarly, nerve growth factor activates TH gene expression via a distinct recognition sequence, in this case an AP-1 site (32).…”

Section: Resultsmentioning

confidence: 99%

Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions.

Choi

Won

Roback

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

MN9D is an immortalized dopaminecontaining neuronal hybrid cell line. When MN9D cells were coaggregated with primary embryonic cells of optic tectum, a brain region that does not receive a dopaminergic innervation, there was a marked reduction in their dopamine content, tyrosine hydroxylase immunoreactivity, and tyrosine hydroxylase mRNA. Similar reductions in dopamine content were produced by coaggregation with cells from embryonic thalamus, another brain region devoid ofdopaminergic innervation.Coaggregation of MN9D cells with dopaminoceptive cells from the corpus striatum or the cortex did not have a demonstrable stimulator effect on the dopamine content of MN9D cells. The decrease in MN9D dopamine content produced by optic tectum cells was not reversed by addition of corpus striatum cells.Thus, the MN9D hybrid cells are able to respond to an inhibitory factor(s) from cells derived from brain areas that are not targets for dopaminergic neurons. Catecholamineproducing PC12 cells did not respond in a similar manner, suggesting that the response ofMN9D cells is a function of their mesencephalic origin. Given the selective response of MN9D cells to different brain cell populations, this hybrid cell line should facilitate investigations of cell-cell interactions in the central nervous system that may be involved in the expression of neurotransmitter phenotype and establishment of specific neuronal connections.Examination of the molecular mechanisms involved in cellcell interactions and establishment of specific neuronal connections in the brain is difficult because of the complexity of the neuronal population. There is, however, considerable evidence for the participation of chemotrophic mechanisms involving soluble molecules in axonal guidance (1) and the direct contact of growth cones with cellular membranes and extracellular matrix (2). In addition to such positive signals, nontarget cells may play a role in inhibition of growth cone extension (3, 4). For dopaminergic neurons, primary tissue cultures have proven useful in approaching some of these issues of neuronal connectivity (5). In particular, the cell aggregation culture system developed by Moscona (6) for examining cell-cell interactions and applied to brain tissue by Garber and Moscona (7) Although the use of cell aggregation techniques simplifies the examination of neuronal interactions, this approach has limitations with respect to identification of the molecules involved because of cell heterogeneity in any brain area and the limited amounts ofmaterial obtainable from a specific cell type for isolation ofspecific molecules. For these reasons, we have developed a series of clonal cell lines using somatic cell fusion (11) that provides large quantities of cells for studies of cell-cell interactions and isolation of specific neurotrophic molecules. Among these cell lines is a clonal catecholamineproducing hybrid cell line designated as MN9D. It was derived from cells of the mesencephalon and contains substantial quantities of dopamine (12). Bri...

show abstract

Section: Resultsmentioning

confidence: 99%

Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions.

Choi

Won

Roback

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In vitro evidence suggests that an immediate effect MB is altered toxicokinetics of DA, such that efflux is impeded, thus increasing DA concentration [Barlow et al, 2003]. During development, DA has autocrine and paracrine functions [De Vitry et al, 1991;Levitt et al, 1997], but excess intracellular DA may be oxidized and interfere with mitochondrial function [Berman and Hastings, 1999]. Acutely, MB exposure increases striatal DA levels in adult mice and rats [Thiruchelvam et al, 2000b;Zhang et al, 2003], MB has also been shown to inhibit mitochondrial complex III [Zhang et al, 2003].…”

Section: Discussionmentioning

confidence: 99%

“…Catecholamine systems, including DA systems, are among the earliest neuronal populations to replicate and differentiate [Johnston, 1985] and are important for further brain development [De Vitry et al, 1991;Levitt et al, 1997]. Due to underdeveloped protective mechanisms and rigid schedules of interconnecting biochemical events, the embryonic nervous system is particularly sensitive to disruption, and may never fully recover after early insult [Rodier, 1994[Rodier, , 1995Gupta et al, 1999;Sobotka et al, 1972].…”

Section: Introductionmentioning

confidence: 99%

A Fetal Risk Factor for Parkinson’s Disease

Barlow¹,

Richfield

Cory‐Slechta

et al. 2004

Dev Neurosci

View full text Add to dashboard Cite

A lack of strong evidence for genetic heritability of idiopathic Parkinson’s disease (PD) has focused attention on environmental toxicants in the disease etiology, particularly agrichemicals. PD is associated with advanced age, but it is unclear whether specific neuronal damage could result from insults during development. This study hypothesized that prenatal exposure to pesticides would disrupt the development of the nigrostriatal dopamine (DA) system and enhance its vulnerability to dopaminergic neurotoxicant exposures later in life. Pregnant C57BL/6J mice were treated on gestational days 10–17 with saline or the pesticides maneb (MB, 1 mg/kg) or paraquat (PQ, 0.3 mg/kg). When offspring were evaluated in adulthood, there were no significant effects of prenatal MB or PQ exposure on locomotor activity. Subsequently, offspring were treated for 8 consecutive days with saline, MB (30 mg/kg), or PQ (5 mg/kg). One week after the last exposure, only males exposed to prenatal MB and adulthood PQ showed significant reductions in locomotor activity (95%) and changes in striatal neurochemistry. Stereological assessment of the substantia nigra pars compacta (SNpc) and ventral tegmental area correspondingly confirmed selective dopaminergic-neuron loss in SNpc. The lack of changes in other exposure groups suggests a specificity to the sequence of exposures as well as gender specificity. These results suggest that prenatal exposure to MB produces selective, permanent alterations of the nigrostriatal dopaminergic system and enhances adult susceptibility to PQ exposure. This study implicates a role for developmental neurotoxicant exposure in the induction of neurodegenerative disorders such as PD.

show abstract

“…Observations on mammalian brain have indicated that the role of some neurotransmitters during ontogeny is completely different from that in the mature brain (Mangoura and Vernadakis, 1988;De Vitry et al, 1991;Ma et al, 1992). In addition to acting as neuromodulators, in fact, they serve as trophic factors regulating the development, maintenance, and plasticity of neuronal morphology (Lipton and Kater, 1989).…”

Section: Functional Meaning Of Gaba Transiencementioning

confidence: 98%

Immunohistochemical investigation of γ‐aminobutyric acid ontogeny and transient expression in the central nervous system of Xenopus laevis tadpoles

et al. 1996

View full text Add to dashboard Cite

The ontogeny of the gamma-aminobutyric acid (GABA)-positive neurons in the brain of Xenopus laevis tadpoles was investigated by means of immunohistochemistry, using specific antibodies both against GABA and its biosynthetic enzyme, glutamate decarboxylase (GAD). The results obtained with the two antisera were comparable. The GABA system differentiates very early during development. At stages 35/36, numerous GABA-positive neurons were seen throughout the prosencephalon and formed two main bilateral clusters within the lateral walls of the forebrain that ran caudally toward the hindbrain. Other GABA-immunolabeled cell bodies, together with a conspicuous network of GABAergic fibers, were seen in the posterior hypothalamus. In the spinal cord, the lateral marginal zone was GABA-positive, as were Rohon-Beard neurons, interneurons, and Kolmer-Agdhur cells. A very rich GABA innervation was observed in the pars intermedia of the pituitary. At stage 50, plentiful immunopositive neurons and fibers were found in the telencephalic hemispheres, the diencephalon, and the mesencephalon (optic tectum and tegmentum). By stage 54, the number of GABA-immunoreactive neurons in the posterior hypothalamus had decreased, so that, at stage 58, there were very few GABA-labeled cell bodies in the dorsolateral walls of the infundibulum, despite a strong GABAergic innervation within the median eminence and the pars intermedia. From stage 58 to stage 66, the distribution pattern was very similar to that described in the adult X. laevis and in other amphibian species. These results point to transient GABA expression within the hypothalamus, possibly related to either 1) a naturally occurring cell death or 2) a phenotypic switch.

show abstract

Dopamine increases the expression of tyrosine hydroxylase and aromatic amino acid decarboxylase in primary cultures of fetal neurons

Cited by 34 publications

References 37 publications

Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions.

Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions.

A Fetal Risk Factor for Parkinson’s Disease

Immunohistochemical investigation of γ‐aminobutyric acid ontogeny and transient expression in the central nervous system of Xenopus laevis tadpoles

Contact Info

Product

Resources

About