Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells

Abstract

DRD1 activation upregulates DRD1 and SULT2A1 expression and SULT2A1 activity in HepG2 cells, suggesting that the DRD1 subtype may be involved in the metabolism of drugs and xenobiotics through regulating SULT2A1.

Exogenous dopamine induces dehydroepiandrosterone sulfotransferase (rSULT2A1) in rat liver and changes the pharmacokinetic profile of moxifloxacin in rats

“…Our results showed that DA enhanced the expression of D1 more than that of D2, and DA increased the cAMP concentrations obviously, suggesting DA performs its functions probably via D1-like receptor linked signaling pathway in rat liver [3]. Besides, our previous results in HepG2 cells showed that attenuation of D1 expression by using siRNA targeting D1 mRNA caused a marked reduction in the overall extent of SULT2A1, and cAMP level also was enhanced by DA in HepG2 cytosol, which is in agreement with our present in vivo results [26].…”

“…In our previous study, D1 agonists (SKF 82958 and SKF38393) were found to markedly increase the mRNA expression of SULT2A1 by about 8-fold and 40-fold in HepG2 cells, respectively, while nonspecific D1/D2 agonist (DA) induced only nearly 2-fold at mRNA level. However, the induction of SULT2A1 at protein and activity level by three compounds are all less than 3-fold [26]. In this study, the strength of rSULT2A1 induction by DA in rat liver was in agreement with that from in vitro study.…”

“…Besides, our previous results showed that methamphetamine, a structural analogue of DA, could induce the expressions of rSULT2A1 remarkably in rat liver and brain [23,24]. Our recent data in vitro also revealed that the activity of SULT2A1 in HepG2 cells increased significantly after the treatment with DA, and the expression of SULT2A1 was significantly induced by specific D1 agonists (SKF-38393 and SKF 82958) that activating D1 signaling pathway selectively [26]. In addition, it has been demonstrated that dopaminergic system could regulate cytochrome P450 (CYP) isoforms in rat liver [27e29].…”

“…Our previous study demonstrated that specific D1 agonists induce SULT2A1 in human HepG2 cells, via the pathway of D1 activation, and all D1-D5 receptors are expressed in HepG2 cells [26]. These results suggest that dopamine receptors maybe function in animal liver as well, just like those in other outer peripheral system [3,6e9].…”

Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex

“…In order to verify that the complexes were interacting with dopamine receptors (D1R/D2R) in cells, siRNA knockdown experiments were performed with D1R/D2R siRNA in A549. 27 – 29 As shown by Western blotting experiments, the levels of both D1R ( Fig. 3a ) and D2R ( Fig.…”