Cell imaging of dopamine receptor using agonist labeling iridium(<scp>iii</scp>) complex

Vellaisamy, Kasipandi; Li, Guodong; Ko, Chung-Nga; Zhong, Hai‐Jing; Fatima, Sarwat; Kwan, Hiu Yee; Wong, Chun‐Yuen; Kwong, Wai-Jing; Tan, Weihong; Leung, Chung‐Hang; Ma, Dik‐Lung

doi:10.1039/c7sc04798c

Cited by 103 publications

(33 citation statements)

References 30 publications

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“…The success of metal complexes such as platinum (Pt) complexes for anticancer applications encourages the development of new metallodrugs . Recent fundamental research and clinical trials showed that ruthenium (Ru) complexes are promising alternatives for Pt drugs .…”

Section: Introductionmentioning

confidence: 99%

Red‐Light‐Controlled Release of Drug–Ru Complex Conjugates from Metallopolymer Micelles for Phototherapy in Hypoxic Tumor Environments

Sun

Yan

Thiramanas

et al. 2018

Adv Funct Materials

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Traditional photodynamic phototherapy is not efficient for anticancer treatment because solid tumors have a hypoxic microenvironment. The development of photoactivated chemotherapy based on photoresponsive polymers that can be activated by light in the "therapeutic window" would enable new approaches for basic research and allow for anticancer phototherapy in hypoxic conditions. This work synthesizes a novel Ru-containing block copolymer for photoactivated chemotherapy in hypoxic tumor environment. The polymer has a hydrophilic poly(ethylene glycol) block and a hydrophobic Ru-containing block, which contains red-light-cleavable (650-680 nm) drug-Ru complex conjugates. The block copolymer self-assembles into micelles, which can be efficiently taken up by cancer cells. Red light induces release of the drug-Ru complex conjugates from the micelles and this process is oxygen independent. The released conjugates inhibit tumor cell growth even in hypoxic tumor environment. Furthermore, the Ru-containing polymer for photoactivated chemotherapy in a tumor-bearing mouse model is applied. Photoactivated chemotherapy of the polymer micelles demonstrates efficient tumor growth inhibition. In addition, the polymer micelles do not cause any toxic side effects to mice during the treatment, demonstrating good biocompatibility of the system to the blood and healthy tissues. The novel red-light-responsive Ru-containing polymer provides a new platform for phototherapy against hypoxic tumors.

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Section: Introductionmentioning

confidence: 99%

Red‐Light‐Controlled Release of Drug–Ru Complex Conjugates from Metallopolymer Micelles for Phototherapy in Hypoxic Tumor Environments

Sun

Yan

Thiramanas

et al. 2018

Adv Funct Materials

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“…As a GPCR (G protein-coupled receptor) member, DR is classified into D1-like (including DRD1 and DRD5) and D2-like (including DRD2, DRD3 and DRD4) families according to their coupled G proteins, Gαs or Gαi [ 17 , 18 ]. Many neurogenic diseases such as Parkinson and Alzheimer’s disease were associated with DR dysfunction and relevant agonists or antagonists are used to target DR for therapy or modified to generate effective probes for live imaging [ 19 , 20 , 21 , 22 , 23 ]. Since Zhang et al reported that compounds with affinity for DRs could modulate autophagy in a screen [ 24 ], functions of different DRs agonists and antagonists in autophagy have been examined in cells and animals [ 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Dopamine Receptor Subtypes Differentially Regulate Autophagy

Wang

Liu

et al. 2018

IJMS

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Some dopamine receptor subtypes were reported to participate in autophagy regulation, but their exact functions and mechanisms are still unclear. Here we found that dopamine receptors D2 and D3 (D2-like family) are positive regulators of autophagy, while dopamine receptors D1 and D5 (D1-like family) are negative regulators. Furthermore, dopamine and ammonia, the two reported endogenous ligands of dopamine receptors, both can induce dopamine receptor internalization and degradation. In addition, we found that AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase) pathways are involved in DRD3 (dopamine receptor D3) regulated autophagy. Moreover, autophagy machinery perturbation inhibited DRD3 degradation and increased DRD3 oligomer. Therefore, our study investigated the functions and mechanisms of dopamine receptors in autophagy regulation, which not only provides insights into better understanding of some dopamine receptor-related neurodegeneration diseases, but also sheds light on their potential treatment in combination with autophagy or mTOR pathway modulations.

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“…This considerably increases image resolution in luminescence microscopy and lowers detection threshold in sensing experiments. 1,4,11,12 As a result, advanced sensing platforms were developed to recognize various biologically relevant analytes, including (but not limited to) detection of cyanide ions, 13 biothiols 14 (including thiourea 15 ), b-galactosidase, 16 dopamine receptor, 17 or inhibitor of tumor necrosis factor-a. 18 Moreover, the sensitivity of emission parameters (intensity and lifetime) to oxygen concentration makes possible mapping of oxygen concentration in in vitro and in vivo experiments, 19,20 including quantitative measurements with the use not only emission intensity but also phosphorescence lifetime imaging (PLIM) techniques, [21][22][23][24][25] which provides considerably higher precision in oxygen biodistribution mapping.…”

Section: Introductionmentioning

confidence: 99%

Water-soluble cyclometalated platinum(ii) and iridium(iii) complexes: synthesis, tuning of the photophysical properties, and in vitro and in vivo phosphorescence lifetime imaging

Solomatina

Lukina

et al. 2018

RSC Adv.

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This paper presents synthesis and photophysical investigation of cyclometalated water-soluble Pt(II) and Ir(III) complexes containing auxiliary sulfonated diphosphine (bis(diphenylphosphino)benzene (dppb), P^P*) ligand. The complexes demonstrate considerable variations in excitation (extending up to 450 nm) and emission bands (with maxima ranging from ca. 450 to ca. 650 nm), as well as in the sensitivity of excited state lifetimes to molecular oxygen (from almost negligible to more than 4-fold increase in degassed solution). Moreover, all the complexes possess high two-photon absorption cross sections

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Cell imaging of dopamine receptor using agonist labeling iridium(iii) complex

Abstract: A long-lived complex 13 could selectively bind to dopamine receptors (D1R/D2R) and monitor their internalization in living cells.

Cited by 103 publications

References 30 publications

Red‐Light‐Controlled Release of Drug–Ru Complex Conjugates from Metallopolymer Micelles for Phototherapy in Hypoxic Tumor Environments

Red‐Light‐Controlled Release of Drug–Ru Complex Conjugates from Metallopolymer Micelles for Phototherapy in Hypoxic Tumor Environments

Dopamine Receptor Subtypes Differentially Regulate Autophagy

Water-soluble cyclometalated platinum(ii) and iridium(iii) complexes: synthesis, tuning of the photophysical properties, and in vitro and in vivo phosphorescence lifetime imaging

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