Dominant lethal effects of nocodazole in germ cells of male mice

Attia, Sabry M.; Ahmad, Sheikh F.; Okash, Radwa Mohamed; Bakheet, Saleh A.

doi:10.1016/j.fct.2015.01.004

Cited by 6 publications

(7 citation statements)

References 32 publications

(34 reference statements)

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“…As the duration of mouse spermatogenesis is 35 days (Attia et al, 2015), many of the evaluated sperm cells had been exposed to the IF field during the whole spermatogenesis and then stored in the cauda epididymis for 3 weeks. The shortest possible exposure was experienced by those cells that had just reached maturity at the time of analysis; such cells were exposed for the two first weeks of spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Effects of intermediate frequency magnetic fields on male fertility indicators in mice

Kumari

Capstick

Cassarà

et al. 2017

Environmental Research

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Human exposure to intermediate frequency (IF) fields is increasing due to new applications such as electronic article surveillance systems, wireless power transfer and induction heating cookers. However, limited data is available on effects of IF magnetic fields (MF) on male fertility function. This study was conducted to assess possible effects on fertility indicators from exposure to IF MF. Male C57BL/6J mice were exposed continuously for 5 weeks to 7.5 kHz MF at 12 and 120 μT. Sperm cells from cauda epididymis were analysed for motility, total sperm counts, and head abnormalities. Motile sperm cells were classified as progressive or non-progressive. Testicular spermatid heads were counted as well. The body weight development and reproductive tissue weights were not affected. No exposure-related differences were observed in sperm counts or sperm head abnormalities. Proportion of non-motile cells was significantly decreased in the 120 µT group, and a corresponding increase was seen in the percentage of motile cells (significant in non-progressive motile cells). In conclusion, no adverse effects on fertility indicators were observed. Increased sperm motility is an interesting finding that needs to be confirmed in further studies.

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Section: Discussionmentioning

confidence: 99%

Effects of intermediate frequency magnetic fields on male fertility indicators in mice

Kumari

Capstick

Cassarà

et al. 2017

Environmental Research

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“…6G). While nocodazole clearly arrests cells in mitosis it is also known to cause DNA damage [58][59][60][61] , and DEK contributes to DNA damage repair. 37 Thus DEK may be retained on chromatin in nocodazole treated cells in order to support the DNA damage response and repair.…”

Section: Discussionmentioning

confidence: 99%

DEK over-expression promotes mitotic defects and micronucleus formation

Matrka

Hennigan

Kappes³

et al. 2015

Cell Cycle

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The DEK gene encodes a nuclear protein that binds chromatin and is involved in various fundamental nuclear processes including transcription, RNA splicing, DNA replication and DNA repair. Several cancer types characteristically over-express DEK at the earliest stages of transformation. In order to explore relevant mechanisms whereby DEK supports oncogenicity, we utilized cancer databases to identify gene transcripts whose expression patterns are tightly correlated with that of DEK. We identified an enrichment of genes involved in mitosis and thus investigated the regulation and possible function of DEK in cell division. Immunofluorescence analyses revealed that DEK dissociates from DNA in early prophase and re-associates with DNA during telophase in human keratinocytes. Mitotic cell populations displayed a sharp reduction in DEK protein levels compared to the corresponding interphase population, suggesting DEK may be degraded or otherwise removed from the cell prior to mitosis. Interestingly, DEK overexpression stimulated its own aberrant association with chromatin throughout mitosis. Furthermore, DEK colocalized with anaphase bridges, chromosome fragments, and micronuclei, suggesting a specific association with mitotically defective chromosomes. We found that DEK over-expression in both non-transformed and transformed cells is sufficient to stimulate micronucleus formation. These data support a model wherein normal chromosomal clearance of DEK is required for maintenance of high fidelity cell division and chromosomal integrity. Therefore, the overexpression of DEK and its incomplete removal from mitotic chromosomes promotes genomic instability through the generation of genetically abnormal daughter cells. Consequently, DEK over-expression may be involved in the initial steps of developing oncogenic mutations in cells leading to cancer initiation

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“…The outcome of studies investigating reproductive failure in rodents exposed to landfill leachates may be useful in understanding how humans residing and working within 2–3 km radius of landfill facilities expressed high incidence of low birth weight, different congenital malformations, adverse pregnancy outcomes and other reproductive or developmental anomalies [ 3 , 15 , 16 ]. In the study herein, a dominant lethal test, an assay that detects mutagens capable of increasing male germ cell mutation during spermatogenesis, embryonic viability and/or present risk of transmissible genetic damage [ 17 ], was used to evaluate embryonic viability in female mice mated with leachate exposed male mice. The study utilizes the sperm head abnormality and dominant lethal mutation test as biomarkers to assess the potentials of OSL to increase reproductive abnormalities and reduce embryonic viability in mice.…”

Section: Introductionmentioning

confidence: 99%

Reproductive toxicity assessment of Olusosun municipal landfill leachate in Mus musculus using abnormal sperm morphology and dominant lethal mutation assays

Ademola

Alimba

Bakare

2020

Environ Anal Health Toxicol

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Mixture of xenobiotics in Olusosun landfill leachates (OSL) induced somatic and germ cell genotoxicity in eukaryotic cells. However, whether the DNA-damaged germ cells can increase reproductive failure, embryotoxicity and/or teratogenicity during fertilization remained unclear. The study herein investigated reproductive toxicity and embryotoxic effects of OSL in mice using abnormal sperm morphology and dominant lethal mutation assays. Six mice per group were exposed to 0.5 mL of 5, 10, 25, and 50% OSL (v/v, leachate/distilled water) for 5 consecutive days. Similar treatment was giving to distilled water and cyclophosphamide (20 mg/kg body weight), as negative and positive control groups, respectively. At 35 day post-treatment, the mice were sacrificed and examined for cauda epididymal abnormal sperm morphology, testicular weight change and histopathological lesions. Male mice were mated to untreated nulliparous females (1 treated male per 2 untreated females) until vaginal plugs were observed. Pregnant females were sacrificed on 14th day post-mating and uterine content examined for number of implants per female, resorptions, and live and dead fetuses. OSL significantly increased teratozoospermia by 31.02% with amorphous sperm head having the highest frequency and sperm with two heads, the least. Histology of the testes revealed congestion of the interstitial blood vessels, spermatid retention and disorganization of the germinal epithelium from the basal compartment. OSL insignificantly increased absolute and relative testes weights but reduced body weight of exposed mice. There was insignificant induction of dominant lethal mutation, although low fertility was observed in the treated mice. The analyzed physico-chemical parameters and heavy metals/metalloids in OSL were higher than permissible limits. The metals, physico-chemical parameters and other unanalyzed substances in OSL induced abnormal sperm morphology, altered testes pathology, but did not significantly induce dominant lethal mutation in mice. Constituents of OSL are toxic to the male reproductive organs in mice and suggests harmful impact on public health. Indiscriminate disposal of solid wastes in the environment should be prevented.

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Dominant lethal effects of nocodazole in germ cells of male mice

Cited by 6 publications

References 32 publications

Effects of intermediate frequency magnetic fields on male fertility indicators in mice

Effects of intermediate frequency magnetic fields on male fertility indicators in mice

DEK over-expression promotes mitotic defects and micronucleus formation

Reproductive toxicity assessment of Olusosun municipal landfill leachate in Mus musculus using abnormal sperm morphology and dominant lethal mutation assays

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