Dominant factors for adsorptive recovery of carnosine based on immobilized metal affinity adsorption

Oshima, Tairo; Sakamoto, Toshihiko; Tachiyama, Hodzumi; Kanemaru, Kenzo; Ohe, Kaoru; Baba, Yoshihiro

doi:10.1016/j.jbiosc.2009.08.190

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the perspective of pI, magG@PDA@Cu 2+ composites were more likely to enrich peptides with a theoretical pI lower than 8.3. Because the immobilization for IMAC is based on the principles of hard and soft acids and bases 43 and the enrichment procedure was performed in alkaline solution (pH = 8.3), the peptides with low pI values became negatively charged under this condition, increasing the chances to interact with the positively charged Cu 2+ .…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu²⁺ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Zhao

Deng

Zhang

2013

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

In this work, Cu(2+)-immobilized magnetic graphene@polydopamine (magG@PDA@Cu(2+)) composites were synthesized for the first time. Magnetic graphene prepared via a hydrothermal reaction were easily encapsulated by a layer of polydopamine through the oxidative polymerization of dopamine in alkaline buffer, and it was conveniently modified with Cu(2+) ions afterward. The as-prepared magG@PDA@Cu(2+) composites were endowed with strong magnetic responsivness, excellent dispersibility and biological compatibility. We applied the novel nanocomposites to the enrichment and identification of low-concentration standard peptides, peptides in standard protein digestions, endogenous peptides in human urine and serum. The enriched peptides were eluted and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The magG@PDA@Cu(2+) composites were proved to exhibit great affinity to both hydrophobic and hydrophilic peptides, thus providing a rapid and facile approach to the extraction of low-concentration peptides. Notably, peptides at an extremely low concentration of 10 pM could be detected by MALDI-TOF MS after enrichment with magG@PDA@Cu(2+) composites. The results demonstrated that the magG@PDA@Cu(2+) composite is a promising candidate for the enrichment of low-abundance peptides for mass spectrometry analysis.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu²⁺ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Zhao

Deng

Zhang

2013

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

Dominant factors for adsorptive recovery of carnosine based on immobilized metal affinity adsorption

Cited by 1 publication

References 0 publications

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu²⁺ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu²⁺ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Contact Info

Product

Resources

About

Dominant factors for adsorptive recovery of carnosine based on immobilized metal affinity adsorption

Cited by 1 publication

References 0 publications

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu2+ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu2+ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Contact Info

Product

Resources

About

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu²⁺ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis

Synthesis of Polydopamine-Coated Magnetic Graphene for Cu²⁺ Immobilization and Application to the Enrichment of Low-Concentration Peptides for Mass Spectrometry Analysis