DOM fluorescence, a tracer for biogeochemical processes in a coastal upwelling system (NW Iberian Peninsula)

Nieto‐Cid, Mar; Álvarez‐Salgado, Xosé Antón; Gago, Jesús; Pérez, Fíz F.

doi:10.3354/meps297033

Cited by 68 publications

(78 citation statements)

References 59 publications

(96 reference statements)

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“…These fixed Ex/Em wavelengths were chosen to assure that we were measuring the same fluorophores in all samples and to allow comparison with other works. Basically, we determined the fluorescence at the wavelengths proposed by Coble et al (1990) and confirmed by Nieto-Cid et al (2005) in the Iberian upwelling system. Four replicate measurements were performed for each Ex/Em wavelength.…”

Section: Methodsmentioning

confidence: 99%

“…Four replicate measurements were performed for each Ex/Em wavelength. A four-points standard curve was prepared daily with a mixed standard of quinine sulphate (QS) and Trp in sulphuric acid 0.05 mol L 21 (Nieto-Cid et al 2005). The equivalent concentration of every peak was determined by subtracting the average peak height from the blank height and dividing by the slope of the standard curve.…”

Section: Methodsmentioning

confidence: 99%

“…These two classes of compounds can be used to trace diverse biogeochemical processes such as labile organic matter production (Nieto-Cid et al 2005), respiration (Chen and Bada 1992;Nieto-Cid et al 2005), and photobleaching (Skoog et al 1996;Moran et al 2000). All these processes play a key role in the accumulation, recycling, and export of DOM in marine ecosystems (Carlson 2002).…”

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confidence: 99%

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Microbial and photochemical reactivity of fluorescent dissolved organic matter in a coastal upwelling system

2006

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We observed significant changes in the dissolved oxygen content and the fluorescence of humic substances and dissolved aromatic amino acids after 24 h light and dark incubations in the coastal upwelling system of the Ría de Vigo under a wide variety of meteorologic and oceanographic conditions. Respiration rates were inversely correlated with the net production of humic fluorescence in the dark at a net rate of 20.027 6 0.003 mg equivalents of quinine sulphate per mmol of O 2 , suggesting that marine humics are a by-product of the bacterial respiration of dissolved organic matter (DOM). On the contrary, humic fluorescence consumption in the light minus dark incubations was positively correlated with the net production in the dark, indicating a rapid photodegradation of recently produced marine humic substances. Parallel incubation experiments demonstrated that daily photodegradation rates and residual humic fluorescence levels followed a seasonal pattern characterized by a marked autumn maximum. Finally, a significant linear correlation between the gross primary production (Pg) and the net production of aromatic amino acids fluorescence in the light pointed to the rapid consumption of dissolved protein-like materials at a net average rate of 21.4 6 0.2 ppb equivalents of tryptophan per day, which accumulates in the water column only when Pg exceeds 80 6 20 mmol kg 21 d 21 .

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Microbial and photochemical reactivity of fluorescent dissolved organic matter in a coastal upwelling system

2006

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“…Such fluorescent data pooled into excitation and emission matrices (EEMs) provide insights into the different types of organic compounds in DOM (Coble et al 1998;Yamashita and Tanoue 2003). In marine systems, a high concentration of protein-like FDOM is often associated with high biological activity in the surface layers (Yamashita and Tanoue 2003;Nieto-Cid et al 2005). The protein-like FDOM is often correlated with dissolved free amino acids (Yamashita and Tanoue 2003), which are readily consumed by bacterioplankton within timescale of hours to several weeks.…”

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confidence: 99%

“…The protein-like FDOM is often correlated with dissolved free amino acids (Yamashita and Tanoue 2003), which are readily consumed by bacterioplankton within timescale of hours to several weeks. Therefore, DOM fluorescence from these compounds has been used to trace dynamics of labile and semilabile organic matter in various aquatic environments (Yamashita and Tanoue 2003;Nieto-Cid et al 2005;Guillemette and del Giorgio 2011). Humic-like fluorescence is mainly due to a heterogeneous mixture of complex biopolymers (humic substances), which constitute , 30-50% of dissolved organic carbon (DOC) in aquatic systems (Thurman 1985).…”

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confidence: 99%

Biogeochemical control on fluorescent dissolved organic matter dynamics in a large freshwater lake (Lake Biwa, Japan)

Thottathil

Hayakawa

Hodoki

et al. 2013

Limnology & Oceanography

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The dynamics of fluorescent dissolved organic matter (FDOM) in the large monomictic freshwater Lake Biwa (surface area 675 km 2 , maximum depth 104 m) was studied from December 2010 to December 2011. The proteinlike FDOM (FDOM T ) and dissolved organic carbon (DOC) showed epilimnetic accumulation (FDOM T from 4.42 6 0.22 quinine sulfate units [QSU] to 6.30 6 0.04 QSU; DOC from 80.8 6 2.7 mmol L 21 to 102.7 6 3.5 mmol L 21 ) between nutrient-replete winter mixing to nutrient-depleted stratified periods. This accumulation is attributed to the reduced heterotrophic activity following severe P-limitation. The positive correlation between accumulated DOC and FDOM T in the epilimnion and their uniform reduction in the hypolimnion (, 9%) suggest FDOM T as a proxy for semi-labile DOM. The humic-like FDOM (FDOM M ) generally increased with depth, a pattern similar to nutrients and total carbon dioxide (TCO 2 ), but adverse to dissolved oxygen. The significant positive correlations of FDOM M with apparent oxygen utilization (r 5 0.86, p , 0.001), TCO 2 (r 5 0.91, p , 0.001), nitrate (r 5 0.83, p , 0.001), and phosphate (r 5 0.76, p , 0.001) in the deeper layers suggest that FDOM M is formed during hypolimnetic mineralization. We estimated that , 8% of the organic carbon degraded in the hypolimnion is transferred into humic substances. The minor contribution of DOC (6.4%) to hypolimnetic mineralization suggests that production of humic substances is mainly fueled by the mineralization of sinking biogenic particles. The production and consumption of FDOM in freshwater lakes may influence the quality and bioavailability of carbon exported from these systems.

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Absorption and fluorescence of dissolved organic matter in the waters of the Canadian Arctic Archipelago, Baffin Bay, and the Labrador Sea

Guéguen

Cuss

Cassels

et al. 2014

J. Geophys. Res. Oceans

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The optical properties of dissolved organic matter (DOM) were investigated for the first time in the Canadian Arctic Archipelago (CAA), Baffin Bay (BB), and Labrador Sea (LS) as part of the International Polar Year Canada's Three Oceans project (C3O). The dynamics and composition of absorbing DOM (i.e., colored DOM, or CDOM) and fluorescent DOM (FDOM) were evaluated in several distinct water masses occupying the three regions: surface waters, Arctic outflow waters, West Greenland Intermediate waters (WGIW), upper Labrador Sea waters (uLSW), and Bottom Baffin Deep Water (BBDW). Four fluorescent components were identified by applying parallel factor analysis (PARAFAC) to 522 excitation emission matrix (EEM) spectra: three humic-like and one protein-like. The FDOM in surface waters of the CAA and BB differed considerably in character from those of the LS, with higher fluorescence intensity in the former. The fluorescence intensities of the two terrestrial humic-like components (C1 and C3) were linearly correlated with apparent oxygen utilization (AOU) in the CAA Arctic outflow and the WGIW whereas only humic-like C3 was significantly correlated with AOU in uLSW. These findings suggest that the humic-like components were produced in situ as organic matter was bio-oxidized. The slopes of the linear relationship between humic-like intensity and AOU were significantly greater in the WGIW relative to the Arctic outflow waters, which implies that FDOM in the Arctic-derived deeper layer was less prone to mineralization.

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DOM fluorescence, a tracer for biogeochemical processes in a coastal upwelling system (NW Iberian Peninsula)

Abstract: ), where labile DOM is produced from phytoplankton exudation and/or lysis and (2) the bottom nepheloid layer (at a rate of about 0.2 ppb eq tryptophan d

Cited by 68 publications

References 59 publications

Microbial and photochemical reactivity of fluorescent dissolved organic matter in a coastal upwelling system

Microbial and photochemical reactivity of fluorescent dissolved organic matter in a coastal upwelling system

Biogeochemical control on fluorescent dissolved organic matter dynamics in a large freshwater lake (Lake Biwa, Japan)

Absorption and fluorescence of dissolved organic matter in the waters of the Canadian Arctic Archipelago, Baffin Bay, and the Labrador Sea

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