Does Water-Jet Force Affect Cryopreserved Adipose-Derived Stem Cells? Evidence of Improved Cell Viability and Fat Graft Survival

Abstract

Background Adipose tissue harvested by liposuctions is an available source of adipose-derived stem cells (ASCs). Water-jet–assisted liposuction is a favorable method for fat collection with little mechanical damage. This study aimed to investigate whether or not the water-jet–assisted liposuction made a difference in the biological characteristics of cryopreserved ASCs and fat graft survival in cell-assisted lipotransfer. Methods Human lipoaspirates were obtained from the abdomen or thighs of 20 female participants for body contouring. A single surgeon randomly harvested 50 mL of adipose tissue by the water-jet–assisted liposuction and the conventional liposuction, respectively. Adipose-derived stem cells were isolated from lipoaspirates and then cryopreserved for 4 weeks. Cryopreserved ASCs were used to examine the surface markers, cell proliferation, migration, and adipogenic differentiation in vitro. The fat survival of ASCs-enriched grafts from different liposuctions was measured in animal models. Results The cryopreserved ASCs with the water-jet assistance had better capacities of cell proliferation, migration, and adipogenic differentiation and achieved a better survival result of ASCs-enriched fat grafting. Conclusions Cryopreservation of ASCs with the water-jet force showed more excellent biological characteristics. The water-jet–assisted liposuction was superior to the conventional liposuction in obtaining ASCs and fat survival of coimplantation with grafts.

Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential

“…Earlier studies have shown a variance of 10% −80% survival of fat grafts that are not augmented with ASCs. The water jet technique has shown promising results with regard to cell viability [12][13][14] and when used in the absence of cell supplementation, it is possible to achieve better fat survival using the water jet technique when compared with manual isolation [14] .…”

Development of Multilayer Mesenchymal Stem Cell Cell Sheets

“…MSCs can be easily isolated from different tissues. Even if bone marrow stem cells are still the MSCs used most often, the invasive procedure to isolate the bone marrow makes it more difficult and more stressful for the donors [52], compared with the use of adipose stem cells that can be easily isolated from liposuction [53,54]. In addition, the number of isolated adipose stromal cells from the liposuction can be 50,000 times higher than the number of bone marrow stem cells isolated from the bone marrow [55][56][57].…”