Does the endometrial gene expression of fertile women vary within and between cycles?

Evans, Gloria; Phillipson, Gregory T.M.; Sykes, Peter; McNoe, Les; Print, Cristin G.; Evans, John J.

doi:10.1093/humrep/dex385

Cited by 18 publications

(13 citation statements)

References 49 publications

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“…The endometrial biopsies from healthy participants were all taken at two time-points (ES and MS) within the same menstrual cycle. A recent study by Evans et al demonstrated that the prior collection of an LH + 2 sample does not affect the general gene expression level of a LH + 7 sample [50]. Their study data suggested that the expression of tested genes exhibited a stable pattern, which was not affected even when sampled twice in one cycle and is valid to predict endometrial receptivity in the subsequent cycles.…”

Section: Discussionmentioning

confidence: 99%

A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

Rekker

Altmäe

Suhorutshenko

et al. 2018

Genes

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The endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about the miRNAs involved in mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early to mid-secretory phase transition and with RIF condition could be reflected in blood miRNA profiles. In total, 116 endometrial and 114 matched blood samples collected from two different population cohorts were subjected to small RNA sequencing. Among fertile women, 91 DE miRNAs were identified in the mid-secretory vs. early secretory endometrium, while no differences were found in the corresponding blood samples. The comparison of mid-secretory phase samples between fertile and infertile women revealed 21 DE miRNAs from the endometrium and one from blood samples. Among discovered novel miRNAs, chr2_4401 was validated and showed up-regulation in the mid-secretory endometrium. Besides novel findings, we confirmed the involvement of miR-30 and miR-200 family members in mid-secretory endometrial functions.

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Section: Discussionmentioning

confidence: 99%

A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

Rekker

Altmäe

Suhorutshenko

et al. 2018

Genes

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“…It must be acknowledged, however, that assessment of reproducibility is limited by the small numbers, the lack of abnormal mid-luteal test results, and use of a natural cycle. Interestingly, the concept of reproducibility of gene expression has been recently tested in six subjects with normal receptivity, comparing gene expression across multiple cycles using a whole-genome microarray (88), though the direct application of these data to the ERA remains uncertain.…”

Section: Endometrial Assessmentmentioning

confidence: 99%

What exactly is endometrial receptivity?

Lessey

Young

2019

Fertility and Sterility

263

166

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Endometrial receptivity is a complex process that provides the embryo with the opportunity to attach, invade, and develop, culminating in a new individual and continuation of the species. The window of implantation extends 3-6 days within the secretory phase in most normal women. In certain inflammatory or anatomic conditions, this window is narrowed or shifted to preclude normal implantation, leading to infertility or pregnancy loss. Of the factors that prevent normal implantation and pregnancy, embryo and endometrial quality share responsibility. In this review, we highlight the advances in the study of implantation from the perspective of the endometrium, normally a barrier to implantation. New advances will allow the early identification of defects in endometrial receptivity and provide new avenues for treatment that promote successful establishment of pregnancy.

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“…Despite recent advances in assisted reproduction techniques (ART), implantation rates remain low after controlled ovarian stimulation (COS) for in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI) treatments (van der Gaast et al, 2008). Embryonic implantation failure remains the major limitation of ART success (Evans et al, 2018;van der Gaast et al, 2008), and although embryo quality is the main determinant of implantation success, maturity and endometrial receptivity are important factors to consider. Embryo implantation followed by gestation depends on a viable and high quality embryo, a receptive endometrium and having a molecular "dialogue" between them (Dieamant et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Recent research in this area has focused on finding a viable endometrial receptivity marker that may help to identify the best moment to perform an embryo transfer (Edgell et al, 2013;Evans et al, 2018;Kliman & Frankfurter, 2019;Miravet-Valenciano et al, 2015;Siristatidis et al, 2018). Histologically, endometrial maturity can be classified using the Noyes criteria (Kliman & Frankfurter, 2019;Noyes et al, 1950;Paulson, 2019), which characterizes the endometrium into its phases (Complementary table).…”

Section: Introductionmentioning

confidence: 99%

Use of clomiphene citrate protocol for controlled ovarian stimulation impairs endometrial maturity

Montenegro¹,

Kuhl²,

Schneider³

et al. 2020

JBRA

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Introduction: Despite recent advances in assisted reproduction techniques and recent knowledge regarding embryo and endometrium quality, implantation and birth rates remain low. The objective of this study was to investigate whether clomiphene citrate alters endometrial maturation in infertile patients. Methods: In a prospective self-matched cohort study, we assessed the ovulation of women in spontaneous and stimulated cycles (with clomiphene citrate). We determined the ovulation day by ultrasound scanning. In both cycles, we took four blood samples (BS1 - at early proliferative phase, BS2 - at mid proliferative phase, BS3 - after ovulation and BS4 - at mid luteal phase) to determine the serum concentrations of FSH, LH, estradiol and progesterone. We retrieved an endometrial biopsy five days after ovulation, followed by blinded analysis and classification according to Noyes criteria, in both cycles. Results: Twenty-two participants completed the study. There were significant differences in FSH BS3 ( p =0.001), in LH BS3 and BS4 ( p <0.001 and p =0.049, respectively), in estradiol BS2, BS3 and BS4 ( p <0.001, p =0.024 and p <0.001, respectively) and in progesterone BS3 and BS4 ( p =0.028 and p <0.001, respectively). Considering Noyes criteria, there was a one-day delay when comparing the stimulated cycle with the spontaneous cycle ( p =0.004), and a two-day delay when comparing the stimulated cycle with the biopsy day. Conclusion: This study indicates that ovarian stimulation with clomiphene citrate delays the endometrial maturity, and could possibly impair the implantation process due to asynchrony.

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Does the endometrial gene expression of fertile women vary within and between cycles?

Abstract: Funding was granted from the Tertiary Education Commission of New Zealand, Contract I.D.:UOOX06007. There are no competing interests.

Cited by 18 publications

References 49 publications

A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

What exactly is endometrial receptivity?

Use of clomiphene citrate protocol for controlled ovarian stimulation impairs endometrial maturity

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