What exactly is endometrial receptivity?

Lessey, Bruce A.; Young, Steven L.

doi:10.1016/j.fertnstert.2019.02.009

Cited by 262 publications

(201 citation statements)

References 91 publications

(91 reference statements)

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“…The fertile window (the time during which a woman may become pregnant) depends upon many factors, including 1) the time of the LH surge, 2) the subsequent time of the release of the ovum or ovulation, 3) the presence of a viable corpus luteum releasing adequate progesterone (Soules et al, 1989), 4) the duration of time sperm can survive in the female body, which is dependent both on sufficient number and quality of sperm and on the appropriate vaginal environment (e.g., pH) (Stanford, 2015;Lessey & Young, 2019), and 5) quality of the uterine environment. Most investigations report the highest probability of fertility as the 5 days preceding ultrasound-determined day of ovulation (USDO) (Faust et al, 2019), but actual days on which an individual may become pregnant are much more variable, with pregnancy occurring 11 days prior to ovulation to 5 days after ovulation (Direito et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Ultradian Rhythms in Heart Rate Variability and Distal Body Temperature Anticipate the Luteinizing Hormone Surge Onset

Grant

Newman²,

Kriegsfeld

2020

Preprint

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The human menstrual cycle is characterized by predictable patterns of physiological change across timescales, yet non-invasive anticipation of key events is not yet possible at individual resolution. Although patterns of reproductive hormones across the menstrual cycle have been well characterized, monitoring these measures repeatedly to anticipate the preovulatory luteinizing hormone (LH) surge is not practical for fertility awareness. In the present study, we explored whether non-invasive and high frequency measures of distal body temperature (DBT), sleeping heart rate (HR), sleeping heart rate variability (HRV), and sleep timing could be used to anticipate the preovulatory LH surge in women. To test this possibility, we used signal processing to examine these measures across the menstrual cycle. Cycles were examined from both pre- (n=45 cycles) and perimenopausal (n=10 cycles) women using days of supra-surge threshold LH and dates of menstruation for all cycles. For a subset of cycles, urinary estradiol and progesterone metabolites were measured daily around the time of the LH surge. Wavelet analysis revealed a consistent inflection point of ultradian rhythm (2-5 h) power of DBT and HRV that enabled anticipation of the LH surge at least 2 days prior to its onset in 100% of individuals. In contrast, the power of ultradian rhythms in heart rate, circadian rhythms in body temperature, and metrics of sleep duration and sleep timing were not predictive of the LH surge. Together, the present findings reveal fluctuations in distal body temperature and heart rate variability that consistently anticipate the LH surge and may aid in fertility awareness.

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Section: Discussionmentioning

confidence: 99%

Ultradian Rhythms in Heart Rate Variability and Distal Body Temperature Anticipate the Luteinizing Hormone Surge Onset

Grant

Newman²,

Kriegsfeld

2020

Preprint

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“…The ovary, embryo, and endometrium undergo complex changes after ovulation. These changes support the onset of implantation and thus the successful establishment of pregnancy (2,9,10). If the ovary fails to produce correct and sufficient levels of signals, or if the uterus fails to respond to those signals, the success of the implantation process and the overall health of any achieved pregnancy can be severely impacted.…”

mentioning

confidence: 99%

Development and utilization of human decidualization reporter cell line uncovers new modulators of female fertility

Haller

Yin

2019

Proc. Natl. Acad. Sci. U.S.A.

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Failure of embryo implantation accounts for a significant percentage of female infertility. Exquisitely coordinated molecular programs govern the interaction between the competent blastocyst and the receptive uterus. Decidualization, the rapid proliferation and differentiation of endometrial stromal cells into decidual cells, is required for implantation. Decidualization defects can cause poor placentation, intrauterine growth restriction, and early parturition leading to preterm birth. Decidualization has not yet been systematically studied at the genetic level due to the lack of a suitable high-throughput screening tool. Herein we describe the generation of an immortalized human endometrial stromal cell line that uses yellow fluorescent protein under the control of the prolactin promoter as a quantifiable visual readout of the decidualization response (hESC-PRLY cells). Using this cell line, we performed a genome-wide siRNA library screen, as well as a screen of 910 small molecules, to identify more than 4,000 previously unrecognized genetic and chemical modulators of decidualization. Ontology analysis revealed several groups of decidualization modulators, including many previously unappreciated transcription factors, sensory receptors, growth factors, and kinases. Expression studies of hits revealed that the majority of decidualization modulators are acutely sensitive to ovarian hormone exposure. Gradient treatment of exogenous factors was used to identify EC50 values of small-molecule hits, as well as verify several growth factor hits identified by the siRNA screen. The high-throughput decidualization reporter cell line and the findings described herein will aid in the development of patient-specific treatments for decidualization-based recurrent pregnancy loss, subfertility, and infertility.

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“…In recent years, the role of the endometrial "maternal side" during early pregnancy is gaining a growing interest and, in particular, the impact of the endometrial receptivity on the pregnancy outcome. Endometrial receptivity refers to a transient period in which ovarian hormones induce changes in endometrial lining in order to allow blastocyst implantation and, in turn, pregnancy initiation [31]. Unfortunately, the "adequate receptive endometrium" is still far from being defined.…”

Section: Discussionmentioning

confidence: 99%

Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin

D’Ippolito

Nicuolo

Papi

et al. 2020

JCM

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Background: Pinopode expression has been suggested as a marker of endometrial receptivity. Methods: We set up an experimental study comparing endometrial tissue from recurrent pregnancy loss (RPL, n = 30) and fertile control (CTR, n = 20) women in terms of pinopode expression/morphology; expression of thrombomodulin (TM) and ezrin; cytoskeletal organization. Endometrial samples were collected during implantation window and evaluated by scanning electron microscopy, western blot, and immunofluorescence. Results: We found that RPL endometrial tissue showed: (i) increased pinopodes density (* p < 0.05); (ii) a reduced diameter of pinopodes (* p < 0.05); (iii) a decreased TM and ezrin expression (p < 0.05). Additionally, confocal images showed a significantly reduced expression of phosphorylated (p)-ezrin, confirming the results obtained through immunoblot analysis. Immunofluorescence staining showed that in CTR samples, junctions between cells are intact and clearly visible, whereas actin filaments appear completely lost in RPL endometrial samples; this suggests that, due to the impaired expression and activity of TM and ezrin, actin does not bind to plasma membrane in order to orchestrate the cytoskeletal actin filaments. Conclusions: Our findings suggest that an impaired expression of TM and expression/activation of ezrin may affect the connection between the TM and actin cytoskeleton, impairing the organization of cytoskeleton and, eventually, the adequate pinopode development.

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What exactly is endometrial receptivity?

Cited by 262 publications

References 91 publications

Ultradian Rhythms in Heart Rate Variability and Distal Body Temperature Anticipate the Luteinizing Hormone Surge Onset

Ultradian Rhythms in Heart Rate Variability and Distal Body Temperature Anticipate the Luteinizing Hormone Surge Onset

Development and utilization of human decidualization reporter cell line uncovers new modulators of female fertility

Expression of Pinopodes in the Endometrium from Recurrent Pregnancy Loss Women. Role of Thrombomodulin and Ezrin

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