Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens?

Faramarzi, Azita; Aghaz, Farank; Jahromi, Masoumeh Golestan; Bakhtiari, Mitra; Khazaei, Mozafar

doi:10.1007/s10561-019-09779-2

Cited by 11 publications

(13 citation statements)

References 32 publications

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“…Also, we studied different doses of vitrification and thawing media supplementation by C. siliqua on sperm quality in young men. Our previous work showed that 20 μg/ml C. siliqua improved sperm quality in these men [20].…”

Section: Discussionmentioning

confidence: 79%

“…To the best of our knowledge, there are no publications on the effects of C. siliqua on cryopreservation in aged men. Although, we showed in our previous research that it has protective effects on sperm cryopreservation in young normozoospermic men [20]. Therefore, in the present study, we assessed the effect of in vitro application of C. siliqua on sperm parameters and chromatin quality after vitrification in aged normozoospermic patients.…”

Section: Introductionmentioning

confidence: 83%

“…Every sample was divided into four aliquots to form subsequent groups: fresh (group I), cryopreserved without treatment (group II), cryopreserved + vitrification medium supplementation with 20 μg/ml C. siliqua (group III), cryopreserved without treatment but thawing medium supplementation with 20 μg/ml C. siliqua for 30 min incubation (group IV). This dose was according to previous research [20]. All assessments were done before cryopreservation and after thawing.…”

Section: Methodsmentioning

confidence: 99%

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In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

Faramarzi

Aghaz

Bakhtiari

et al. 2019

Middle East Fertil Soc J

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Background Vitrification is the main technique in the assisted reproductive technique (ART) labs. Sperm vitrification exposes sperm to damage. The aim of the present study was to evaluate the in vitro effect of Ceratonia siliqua (C. siliqua) application on sperm parameters and chromatin quality in normozoospermic aged men. Semen samples (n = 40) were collected from normozoospermic men over 45 years old. Each specimen was divided into four aliquots to form the subsequent groups: fresh (group I), vitrification without treatment (group II), vitrification with the medium supplemented by 20 μg/ml C. siliqua (group III), and vitrification with the thawing medium supplemented by 20 μg/ml C. siliqua (group IV). Sperm progressive motility, normal morphology and viability were assessed. Also, sperm chromatin quality was evaluated by aniline blue (AB), toluidine blue (TB), and sperm chromatin dispersion (SCD) staining. Results Vitrification caused a significant decrease in sperm progressive motility, normal morphology and viability as well as chromatin quality compared to fresh samples (p < 0.05). Supplementation of vitrification/thawing medium with C. siliqua significantly improved sperm progressive motility, normal morphology, viability, and chromatin quality compared to vitrification without any supplementation (p < 0.05). Conclusions The study showed that C. siliqua can improve the detrimental effect of vitrification on sperm parameters and chromatin quality of normozoospermic aged men.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

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In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

Faramarzi

Aghaz

Bakhtiari

et al. 2019

Middle East Fertil Soc J

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“…The various antioxidants have been useful in fertility preservation to counteract elevated ROS production and maintain the sperm motility, viability and function such as zinc, selenium, vitamins C and E (Galatioto et al, 2008; Iovine et al, 2021; Sinclair, 2000). Recently, several studies have showed that addition of synthetic or natural antioxidants to cryopreservation media reduced detrimental effects of the sperm cryopreservation (Dashtestani et al, 2018; Faramarzi et al, 2019; Keshtgar et al, 2020; Najafi et al, 2019; Santonastaso et al, 2021; Yan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

L‐proline as a novel additive to cryopreservation media improved post‐thaw quality of human spermatozoon via reducing oxidative stress

et al. 2021

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Sperm cryopreservation as a routine technique in assisted reproductive technique (ART) laboratories has detrimental effects on spermatozoa. Various methods have been introduced to improve it. The aim of this research was to evaluate the effects of L‐proline supplementation in cryopreservation medium on normozoospermic semen samples. A total of 30 semen samples were collected from normozoospermic men. Cryopreservation media were supplemented with different concentrations of L‐proline (0, 1, 2 and 4 mmol/L). The semen samples were cryopreserved. After thawing, sperm parameters and chromatin integrity (aniline blue (AB), toluidine blue (TB), sperm chromatin dispersion test (SCD) and chromomycin A3 (CMA3)), reactive oxygen species (ROS), and total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were evaluated. A total of 4 mmol/L L‐proline significantly improved progressive motility and viability (p < 0.05). MDA and ROS levels significantly diminished in samples were cryopreserved by 4 mmol/L L‐proline supplemented cryopreservation media (p < 0.001). Also, it significantly increased TAC level. Also, chromatin damages (AB, TB and CMA3) significantly improved in samples were cryopreserved by 4 mmol/L L‐proline supplemented cryopreservation media (p < 0.05). The results support that the usage of L‐proline supplemented cryopreservation media to improve sperm quality after cryopreservation.

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“…This plant extract has been employed in human spermatozoa [ 54 ] by adding it to the freezing and thawing medium. The range of concentrations used was from 5 to 30 µg/mL.…”

Section: Herbal Pharmacognosy and Its Use In Andrologymentioning

confidence: 99%

Plant Extracts as Alternative Additives for Sperm Preservation

Ros-Santaella

Pintus

2021

Antioxidants

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Sperm preservation is a crucial factor for the success of assisted reproductive technology (ART) in humans, livestock, and wildlife. Irrespective of the extender and the storage conditions used, semen handling and preservation negatively affect sperm quality. Moreover, oxidative stress, which often arises during semen storage, significantly reduces sperm function and compromises the sperm fertilizing ability by inducing oxidative damage to proteins, lipids, and nucleic acids. Plant extracts have recently emerged as a cheap and natural source of additives to preserve and enhance sperm function during semen storage. The present work provides an update on the use of these natural compounds as alternative additives for sperm preservation in 13 animal species, including humans. A detailed description of the effects of 45 plant species, belonging to 28 families, on sperm function during semen storage is presented. The plant material and extraction method employed, dosage, possible toxic effects, and antimicrobial properties are provided.

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Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens?

Cited by 11 publications

References 32 publications

In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men

L‐proline as a novel additive to cryopreservation media improved post‐thaw quality of human spermatozoon via reducing oxidative stress

Plant Extracts as Alternative Additives for Sperm Preservation

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