Does N‐acetyl Cystein Affect the Sensitivity and Specificity of <i>Helicobacter pylori</i> Stool Antigen Test?

Demırtürk, Levent; Yazgan, Yusuf; Tarçın, Özlem; Özel, Melih; Diler, Muhittin; Öncül, Oral; Yıldırım, Şükrü

doi:10.1046/j.1523-5378.2003.00132.x

Cited by 18 publications

(10 citation statements)

References 21 publications

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“…A high prevalence of Helicobacter spp . has previously been reported in pet dogs and cats with gastritis and so our present data confirms these findings [ 18 24 ].…”

Section: Discussionsupporting

confidence: 93%

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Comparison of three diagnostic assays for the identification ofHelicobacter spp. in laboratory dogs

et al. 2015

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A number of Helicobacter species may confound experimental data because of their association with disease progressing in various kinds of laboratory animals. Screening of Helicobacter species is particularly desirable, because they are prevalent in commercial and research animal facilities. The aim of the present study was to compare three diagnostic methods [e.g. Helicobacter stool antigen kit (HpSA), polymerase chain reaction (PCR) and rapid urease test (RUT)] for the identification of Helicobacter spp. in stools or gastric biopsy specimens collected from eight dogs suffering from gastritis. The gastroscopic biopsy specimens were tested using RUT and PCR, while stool specimens were evaluated using both HpSA and PCR. DNAs from the gastric biopsies and stool specimens were analyzed by both a consensus PCR that amplified the RNA polymerase beta-subunit-coding gene (rpoB) of Helicobacter spp. and a species-specific PCR to amplify the urease B gene of Helicobacter heilmannii, Helicobacter pylori, and Helicobacter felis. Helicobacter spp. were detected in 62.5% of the dogs, while H. heilmannii and H. felis were identified in 37.5 and 25% of the dogs, respectively. The HpSA did not efficiently detect Helicobacter spp. in the stool samples compared to the RUT and PCR assays, both of which successfully detected Helicobacter spp. in the two sample types. Finally, we recommend that consensus PCR with stool specimens could be used before the species-specific PCR for identifying Helicobacter species in laboratory dogs.

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“…A high prevalence of Helicobacter spp . has previously been reported in pet dogs and cats with gastritis and so our present data confirms these findings [ 18 24 ].…”

Section: Discussionsupporting

confidence: 93%

“…from the intestine of the animal may vary with time. Furthermore, use of Nacetylcysteine-like mucolytic agents may decrease the accuracy of the diagnosis [ 24 ]. Cut off titer, though difficult to decide but crucial to reach the conclusion by using antigen detection technique [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of three diagnostic assays for the identification ofHelicobacter spp. in laboratory dogs

et al. 2015

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“…Indeed, in experimentally spiked specimens, the lowest concentration could not be detected after only a 24-h delay (388). Treatment with the mucolytic agent N-acetylcysteine has been shown to decrease both the test's sensitivity and specificity (104). As previously stated, determining a cutoff to define a positive sample is crucial, especially for HpSA, and adjustments may have to be made.…”

Section: Stool Testsmentioning

confidence: 98%

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

2007

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SUMMARY The discovery of Helicobacter pylori in 1982 was the starting point of a revolution concerning the concepts and management of gastroduodenal diseases. It is now well accepted that the most common stomach disease, peptic ulcer disease, is an infectious disease, and all consensus conferences agree that the causative agent, H. pylori, must be treated with antibiotics. Furthermore, the concept emerged that this bacterium could be the trigger of various malignant diseases of the stomach, and it is now a model for chronic bacterial infections causing cancer. Most of the many different techniques involved in diagnosis of H. pylori infection are performed in clinical microbiology laboratories. The aim of this article is to review the current status of these methods and their application, highlighting the important progress which has been made in the past decade. Both invasive and noninvasive techniques will be reviewed.

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“…However, the lowest concentration could not be detected after only a 24-hour delay in experimentally spiked specimens 20. Treatment with the mucolytic agent N-acetylcysteine decreases both the specificity and sensitivity 21. While the test is quite specific, it is possible that rare Helicobacter species present in stools may also be detected.…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test

Kim¹,

Kim²,

Park³

et al. 2013

Gut Liver

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Background/AimsThe objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans.MethodsSixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation.ResultsThirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively.ConclusionsThe monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.

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Does N‐acetyl Cystein Affect the Sensitivity and Specificity of Helicobacter pylori Stool Antigen Test?

Cited by 18 publications

References 21 publications

Comparison of three diagnostic assays for the identification ofHelicobacter spp. in laboratory dogs

Comparison of three diagnostic assays for the identification ofHelicobacter spp. in laboratory dogs

Helicobacter pyloriDetection and Antimicrobial Susceptibility Testing

Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test

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