Does Microfluidic Sperm Sorting Affect Embryo Euploidy Rates in Couples with High Sperm DNA Fragmentation?

Keskin, Müge; Pabuçcu, Emre Göksan; Arslanca, Tufan; Demirkiran, Özgür Doğuş; Pabuçcu, Recai

doi:10.1007/s43032-021-00784-z

Cited by 14 publications

(8 citation statements)

References 55 publications

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“…Similarly, in our study, we found that male age is an independent risk factor for spontaneous abortion in ART patients [29]. With the increase of age, there are a variety of factors associated with increasing age that will destroy the DNA replication and repair mechanism and increase the possibility of DNA breakage, causing abortions, which leads to abortion [30,31]. The relationship between male age and the probability of spontaneous abortions could provide genetic counseling for older men.…”

Section: Discussionsupporting

confidence: 72%

Nomogram model to predict the probability of spontaneous abortion in human assisted reproductive technology

Duan¹,

li²,

Ma³

et al. 2023

Preprint

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Background: Spontaneous abortion is one of the most common complications of pregnancy in assisted reproductive technology (ART), it has become a thorny issue that poses a threat to pregnant women and their unborn children. Prevention is more important than treatment as for spontaneous abortion, Unfortunately, there is no accurate model to predict and prevent its occurrence. Methods: This retrospective cohort study evaluated clinical data from 15,723 patients successfully conceived who underwent IVF/ICSI in the first people’s hospital of shangqiu and the affiliated hospital of zhengzhou university from december 2015 to February 2021. Patients were divided into an "abortion group" and a "non-abortion group" according to the outcome of abortion. We further performed a multi-factor and single-factor logistic regression analysis to determine the independent factors, then we establish nomogram model to avoid the occurrence of spontaneous abortion in ART patients based on the independent influencing factors. Results: In this study, 15,723 patients had successfully conceived with ART, of which 2,336 patients (14.86%) had abortion, and 13,387 patients (85.14%) did not. Logistic regression analysis showed that female age (OR 1.050; 95% CI 1.029 ~ 1.061; P<0.001), male age (OR 1.102; 95% CI 1.086 ~ 1.115; P<0.001), follicle-stimulating hormone (OR 1.044; 95% CI 1.015 ~ 1.069; P<0.001), anti-Mullerian hormone (OR 0.891; 95% CI 0.862 ~ 0.925; P<0.001) were risk factors for spontaneous abortion. Then a nomogram model of abortion occurrence was constructed according to the above factors, and the AUC of the modeling group is 0.746 (95%CI 0.707-0.784), the AUC of the verification group is 0.718 (95%CI 0.999-0.737). Conclusions: Our study found that female age, male age, follicle-stimulating hormone, anti-mullerian hormone were independent risk factors for spontaneous abortion, and the Nomogram model is an effective, simple and intuitive clinical model for predicting the probability of spontaneous abortion in ART.

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Section: Discussionsupporting

confidence: 72%

Nomogram model to predict the probability of spontaneous abortion in human assisted reproductive technology

Duan¹,

li²,

Ma³

et al. 2023

Preprint

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“…In a study reporting ICSI outcomes in 97 men with excessive DNA fragmentation assessed by flow cytometry, the miscarriage rate was higher in the extreme SDFi group (24.5% vs 36.8%, for SDFi <15% vs SDFi >50%, respectively; p >0.05) but the cumulative pregnancy rate did not differ between the groups (46.5% vs 48.7%; p >0.05) [ 76 ]. Following delicate techniques for the selection of highly motile sperm with low levels of fragmentation before ART may not provide any benefit in terms of embryonal formation and clinical miscarriage, or live birth rates [ 77 ]. In the same concept, early reports of antioxidant treatment in men undergoing ART have not shown a significant impact on the miscarriage rate, although a favourable effect is seen on the viable pregnancy rate [ 78 ].…”

Section: Resultsmentioning

confidence: 99%

Recurrent miscarriage and male factor infertility: diagnostic and therapeutic implications. A narrative review.

2023

Cent European J Urol

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Introduction Recurrent miscarriage is defined as 2 or more failed clinical pregnancies, typically known as repeated pregnancy loss, occurring before 20 gestational weeks, and further categorized into primary and secondary types. It represents a common and distressing condition to deal with in the field of reproductive medicine, usually affecting <5% of couples, with up to 50% of cases lacking a clearly defined aetiology. The epidemiology also varies depending on maternal age. Remarkably, the situation significantly afflicts expecting parents, whereas maternal factors, such as age and previous pregnancy loss rate, are commonly reported as risk factors. Although previously underestimated, existing evidence suggests the male factor is a possible cause of recurrent pregnancy loss. Material and methods A non-systematic literature review was conducted in the PubMed and Scopus databases for articles written in English investigating the possible association of the male factor in recurrent pregnancy loss. The eligible studies were synthesized in a narrative review format upon discussion and consensus among the authors after being previously independently assessed and selected. Results Lifestyle, obesity, genetic predisposition, chromosomal anomalies, endocrine dysfunction, anatomical abnormalities, immunological factors, infections, and oxidative stress can result in poor embryo development and recurrent miscarriage. Although professional organizations currently recognize male gender as a possible risk factor, specific recommendations on the diagnostic and therapeutic field are still lacking, and the condition necessitates a high level of suspicion and case-by-case management. Conclusions In this review, we delve deeper into the contribution of the male factor in the concept of recurrent miscarriage.

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“…Keskin et al (2022) investigated whether utilizing MSS improves embryo quality and euploidy rates compared to DGC in couples with repeated implantation failure (RIF) and high SDF. They found blastocyst formation was significantly increased ( p < 0.001), but not other measures (fertilization rates, euploidy rates, live births) [ 35 ]. A study by Anderson et al (2020) found significantly increased euploidy rates for day 5 embryos ( n = 358) for FERTILE PLUS™ sperm preparation (63%) compared to DGC (56%; p < 0.05) but no significant difference in pregnancy outcomes.…”

Section: Discussionmentioning

confidence: 99%

Sperm Preparation with Microfluidic Sperm Sorting Chip May Improve Intracytoplasmic Sperm Injection Outcomes Compared to Density Gradient Centrifugation

Banti,

Van Zyl,

Kafetzis

2024

Reprod. Sci.

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Does sperm preparation using the FERTILE PLUS™ Sperm Sorting Chip improve fertilization rates, blastocyst formation, utilization, and euploidy rates in patients undergoing intracytoplasmic sperm injection (ICSI), compared with density gradient centrifugation (DGC)? A single-cohort, retrospective data review including data from 53 couples who underwent ICSI cycles within a 12-month period. For each couple, the two closest, consecutive cycles were identified, where one used the standard technique of sperm preparation (DGC) and the subsequent used FERTILE PLUS™, therefore, couples acted as their own controls. Paired samples t-test was used to compare means for the outcomes (fertilization, blastocyst formation, utilization, and euploidy rates). Binary logistic regression analysis assessed the relationship between female age, the presence of male factor infertility, and euploidy rates. Blastocyst, utilization, and euploidy rates were significantly higher for cycles using FERTILE PLUS™ compared to DGC (76% vs 56%, p = 0.002; 60% vs 41%, p = 0.005, and 40% vs 20%, p = 0.001, respectively). Although there was an increase in fertilization rates for cycles using FERTILE PLUS™, this was not significant (72% vs 68%, p = 0.449). The euploidy rates of females ≤ 35 years were significantly increased when the FERTILE PLUS™ sperm preparation method was used, compared to the older age group (OR 2.31, p = 0.007). No significant association was found between the presence or absence of male factor infertility and euploidy rates between the two cycles. This study provides tentative evidence that the FERTILE PLUS™ microfluidic sorting device for sperm selection can improve blastocyst formation, utilization, and euploidy rates following ICSI in comparison to the DGC method.

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Does Microfluidic Sperm Sorting Affect Embryo Euploidy Rates in Couples with High Sperm DNA Fragmentation?

Cited by 14 publications

References 55 publications

Nomogram model to predict the probability of spontaneous abortion in human assisted reproductive technology

Nomogram model to predict the probability of spontaneous abortion in human assisted reproductive technology

Recurrent miscarriage and male factor infertility: diagnostic and therapeutic implications. A narrative review.

Sperm Preparation with Microfluidic Sperm Sorting Chip May Improve Intracytoplasmic Sperm Injection Outcomes Compared to Density Gradient Centrifugation

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