Does age pay off? Effects of three-generational experiments of nanodiamond exposure and withdrawal in wild and longevity-selected model animals

Augustyniak, Maria; Babczyńska, Agnieszka; Dziewięcka, Marta; Flasz, Barbara; Karpeta-Kaczmarek, Julia; Kędziorski, Andrzej; Mazur, B.; Rozpędek, Katarzyna; Alian, Reyhane Seyed; Skowronek, Magdalena; Świerczek, Ewa; Świętek, Agata; Tarnawska, Monika; Wiśniewska, Klaudia; Ziętara, Patrycja

doi:10.1016/j.chemosphere.2022.135129

Cited by 5 publications

(13 citation statements)

References 66 publications

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“…At the same time, queens showed higher levels of catalase (CAT), and peroxiredoxin (Prx) compared to workers [77]. Our previous studies on A. domesticus show that lines D and H (without additional stress load) do not differ in terms of basal CAT activity and total antioxidant capacity (TAC) and, at the same time, have a higher percentage of cells with reactive oxygen species (ROS+), and cells manifesting signs of apoptosis and/or autophagy [34]. This observation supports the conclusion that a high concentration of CAT may not be significant for extending the life span of D-strain individuals.…”

Section: Selection For Longevity and Susceptibility To Dna Damage Cau...mentioning

confidence: 94%

“…Gut tissues were used for flow cytometry, and prepared as described elsewhere [16,31,34]. The cell suspension was treated according to the protocol supplied with the kit (Luminex, Austin, TX USA; MCH200107).…”

Section: Tissue Preparation For Flow Cytometrymentioning

confidence: 99%

“…The results were presented as dot plot, based on the following cell populations: pATM (−) and pH2A.X (−) cells (no DNA damage), pATM (+) and pH2A.X (−) cells (ATM activation), pATM (−) and pH2A.X (+) cells (H2A.X activation) and dual activation of ATM and H2A.X-cells with double-strand breaks. The measurements were performed as described previously [34]. 4.7.2.…”

Section: Tissue Preparation For Flow Cytometrymentioning

confidence: 99%

“…This study was aimed to evaluate DNA damage and elements of the repair pathway after using low doses of GO, with particular emphasis on different stages of life and potentially different coping capabilities with oxidative stress (resulting from multi-generational selection for longevity and different survival strategies). To achieve this goal, we used a unique strain of the selected model organism Acheta domesticus, which is characterized by several modifications indicating that the extension of life is accompanied by a shift in the redox balance [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus

Flasz

Dziewięcka

Ajay

et al. 2022

IJMS

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The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms.

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Section: Selection For Longevity and Susceptibility To Dna Damage Cau...mentioning

confidence: 94%

Section: Tissue Preparation For Flow Cytometrymentioning

confidence: 99%

Section: Tissue Preparation For Flow Cytometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus

Flasz

Dziewięcka

Ajay

et al. 2022

IJMS

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“…Additionally, they have been shown to have low toxicity, making them a potential alternative to other nanomaterials that may have harmful effects. Overall, using NDs in biomedicine is a rapidly growing field with exciting potential for future advancements [28][29][30]. Some nanoparticles have demonstrated the capacity to augment the stability and bioavailability of diverse therapeutic agents, thereby facilitating precision-targeted and controlled drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Does Selection for Longevity in Acheta domesticus Involve Sirtuin Activity Modulation and Differential Response to Activators (Resveratrol and Nanodiamonds)?

Ziętara,

Flasz,

Augustyniak

2024

IJMS

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Sirtuins, often called “longevity enzymes”, are pivotal in genome protection and DNA repair processes, offering insights into aging and longevity. This study delves into the potential impact of resveratrol (RV) and nanodiamonds (NDs) on sirtuin activity, focusing on two strains of house crickets (Acheta domesticus): the wild-type and long-lived strains. The general sirtuin activity was measured using colorimetric assays, while fluorescence assays assessed SIRT1 activity. Additionally, a DNA damage test and a Kaplan–Meier survival analysis were carried out. Experimental groups were fed diets containing either NDs or RV. Notably, the long-lived strain exhibited significantly higher sirtuin activity compared to the wild-type strain. Interestingly, this heightened sirtuin activity persisted even after exposure to RVs and NDs. These findings indicate that RV and NDs can potentially enhance sirtuin activity in house crickets, with a notable impact on the long-lived strain. This research sheds light on the intriguing potential of RV and NDs as sirtuin activators in house crickets. It might be a milestone for future investigations into sirtuin activity and its potential implications for longevity within the same species, laying the groundwork for broader applications in aging and lifespan extension research.

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A Review on Interaction of Nanomaterials of Group-XIV (G14) Elements of the Periodic Table with Proteins and DNA: Applications in Biotechnology and Pharmacy

Hekmat,

Haertlé,

Leblanc

et al. 2024

BioNanoSci.

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Does age pay off? Effects of three-generational experiments of nanodiamond exposure and withdrawal in wild and longevity-selected model animals

Cited by 5 publications

References 66 publications

Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus

Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus

Does Selection for Longevity in Acheta domesticus Involve Sirtuin Activity Modulation and Differential Response to Activators (Resveratrol and Nanodiamonds)?

A Review on Interaction of Nanomaterials of Group-XIV (G14) Elements of the Periodic Table with Proteins and DNA: Applications in Biotechnology and Pharmacy

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