Does Adding Silver Nanoparticles to Leukocyte- and Platelet-Rich Fibrin Improve Its Properties?

Abstract

Objectives Leucocyte- and platelet-rich fibrin (L-PRF) membrane can be used in various regenerative treatments. In the case of classical heterologous membrane exposure, microorganisms can be colonized on it and jeopardize the success of treatment. The aim of this study was to compare the antibacterial, mechanical, and histologic characteristics of the L-PRF membrane before and after the addition of silver nanoparticles (SNP). Materials and Method This study was performed on 10 volunteer men aged 25-35 years. 20 ml whole bloods were collected from each person and L-PRFs were made by routine and SNP modified method. Mechanical, antibacterial, and histological properties were evaluated. Results The antibacterial efficacy of L-PRF and nanosilver-modified L-PRF was presented as Klebsiella pneumonia had growth on the L-PRF membrane after 12 hours. After 24 hours, Klebsiella pneumonia and Streptococcus viridans had growth on L-PRF and only Klebsiella pneumonia had growth on SNP-L-PRF. The tensile strength and stiffness were significantly higher in the SNP-L-PRF. Precipitation of the SNPs was patchy in the outer layers and quite homogeneous in the inner core. Conclusion Modification of L-PRF with SNP improves the mechanical properties and antibacterial activity of the L-PRF. It can play an important role in regenerative procedures.

Mechanical and structural properties of leukocyte‐ and platelet‐rich fibrin membranes: An in vitro study on the impact of anticoagulant therapy

“…The E‐modulus of the control membranes in the current study was 0.07 MPa and is comparable (Table 4) to the results of one study found in literature., 15 but lower than the results reported in two other studies and higher than a fourth study 16‐18 . The ultimate tensile strength of the control membranes was 0.29 MPa, which is higher than reports of the strength of L‐PRF in multiple other studies 15,17‐19 . The elongation at break of the control membranes in the current study was about 2.8 times their initial length, which is higher, comparable, and lower than three other studies that recorded an elongation of 1.45, 2.17, and ± 3.50, respectively 15,16,19 .…”

“…In short, the current results of tensile testing are quite similar or differ slightly from results obtained in other studies 15,17‐19 . All previously mentioned studies had different protocols for blood centrifugation and tensile testing though, 15‐19 which makes any comparison difficult (Table 4). The time of blood centrifugation, the g‐force of the centrifuge, the shape of the L‐PRF membrane (rectangular or dog bone shaped) with tensile testing and the crosshead speed of the tensile test have to be taken into consideration.…”

“…The elongation at break of the control membranes in the current study was about 2.8 times their initial length, which is higher, comparable, and lower than three other studies that recorded an elongation of 1.45, 2.17, and ± 3.50, respectively 15,16,19 . In short, the current results of tensile testing are quite similar or differ slightly from results obtained in other studies 15,17‐19 . All previously mentioned studies had different protocols for blood centrifugation and tensile testing though, 15‐19 which makes any comparison difficult (Table 4).…”

“…Few studies report on the mechanical properties of L‐PRF membranes. The E‐modulus of the control membranes in the current study was 0.07 MPa and is comparable (Table 4) to the results of one study found in literature., 15 but lower than the results reported in two other studies and higher than a fourth study 16‐18 . The ultimate tensile strength of the control membranes was 0.29 MPa, which is higher than reports of the strength of L‐PRF in multiple other studies 15,17‐19 .…”

Does PRP Improve the Tendon Gap Healing?

“…The third layer (PRP) was carefully separated by pipette and recentrifuged at 1240 rpm for 5 minutes. Then, the plasma layer (top layer) was discarded and the PRP layer (second layer) was collected for intra conduit injection (7,8).…”