Does a stable isotopically labeled internal standard always correct analyte response?

Wang, Sherry; Cyronak, Matthew J.; Yang, Eric

doi:10.1016/j.jpba.2006.08.010

Cited by 360 publications

(146 citation statements)

References 15 publications

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“…Standard addition [22], echo-peak technique [23,24], isotope dilution [25], post-column standard infusion [26], internal standard usage [27] and matrix-matched calibration [28] can be done to reduce the influence of matrix effect. However, this group of approaches is not immune to matrix effect when using ESI interface.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography‐electrospray tandem mass spectrometry and the evaluation of the matrix effect

Fan

Wang

et al. 2011

J of Separation Science

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In the experiment, a high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with selected reaction monitoring was used to simultaneously determine various classes of phytohormones, including indole-3-acetic acid, α-naphthaleneacetic acid, 2-chlorobenzoic acid, 4-chlorobenzoic acid, indole-3-butyric acid, gibberellic acid, 2,4-dichlorophenoxyacetic acid, 2-naphthoxyacetic acid, abscisic acid, 2,3,5-triiodobenzoic acid, uniconazole, paclobutrazol and 2,4-epibassinolide in rape tissues. The analyses were separated by an HPLC equipped with a reversed-phase column using a binary solvent system composed of methanol and water, both containing 0.1% of formic acid. The matrix effect was also considered and determined. The technology was applied to analyze rape tissues, including roots, stems, leaves, flowers, immature pods and rape seeds. The rape tissues were subjected to ultrasound-assisted extraction and purified by dispersive solid-phase extraction, and then transferred into the liquid chromatography system. The detection limit for each plant hormone was defined by the ratio of signal/background noise (S/N) of 3. The results showed perfect linearity (R(2) values of 0.9987-1.0000) and reproducibility of elution times (relative standard deviations, RSDs,<1%) and peak areas (RSDs,<7%) for all target compounds.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography‐electrospray tandem mass spectrometry and the evaluation of the matrix effect

Fan

Wang

et al. 2011

J of Separation Science

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show abstract

“…[26][27][28] Evaluations based on the relative signal intensities using internal standards, which are typically stable isotope-labeled analogues or compounds having similar structures to the analytes, are also effective in diminishing the matrix effect, 20,22,23 but this may not always be applicable. 29 Nevertheless, the matrix effect is strongly dependent on the chemical nature of each sample, so there is no universal approach that minimizes or overcomes the matrix effect.…”

Section: ·2 Effects Of the Matrix Effect And On-line Separationmentioning

confidence: 99%

Electrospray Ionization Mass Spectrometry for the Quantification of Inorganic Cations and Anions

Hotta

Tsunoda

2015

ANAL. SCI.

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“…On the other hand, the internal standard had to elute close to the compound of interest, showing similar behavior in the detector, in such a way that more than one of them should be used [77]. It is generally observed that using internal standards derives in significant enhancement of certain analytical figures of merit such as precision, linearity and accuracy.…”

Section: Variations In the Analytical Responsementioning

confidence: 99%

Chemometric strategies for enhancing the chromatographic methodologies with second-order data analysis of compounds when peaks are overlapped

Goicoechea

Culzoni

García

et al. 2011

Talanta

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This overview covers the different chemometric strategies linked to chromatographic methodologies that have been used and presented in the recent literature to cope with problems related to incomplete separation, the presence of unexpected components in the sample, matrix effect and changes in the analytical signal due to pre-treatment of sample.Among the different chemometric strategies it focuses on pre-treatment of data to correct background and time shift of chromatographic peaks and the use of second-order algorithms to cope with overlapping peaks from analytes or from analytes and interferences in liquid chromatography coupled to diode array, fast-scanning fluorescence spectroscopy and mass spectrometry detectors.Finally the review presents the strategies used to deal with changes in the analytical response as result of matrix effect in liquid and gas chromatography, as well as the use of standardization strategies to correct modifications in the analytical signal as a consequence of sample pre-treatment in liquid chromatography.

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Does a stable isotopically labeled internal standard always correct analyte response?

Cited by 360 publications

References 15 publications

Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography‐electrospray tandem mass spectrometry and the evaluation of the matrix effect

Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography‐electrospray tandem mass spectrometry and the evaluation of the matrix effect

Electrospray Ionization Mass Spectrometry for the Quantification of Inorganic Cations and Anions

Chemometric strategies for enhancing the chromatographic methodologies with second-order data analysis of compounds when peaks are overlapped

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