Doença de Chagas crônica: do xenodiagnóstico e hemocultura à reação em cadeia da polimerase

Portela-Lindoso, Ana Angélica Bulcão; Shikanai-Yasuda, Maria Aparecida

doi:10.1590/s0034-89102003000100016

Cited by 68 publications

(28 citation statements)

References 45 publications

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“…Benznidazole is being increasingly prescribed for the antiparasitic treatment of Chagas disease, not only the expansion of indications based on evidence, but also as a result of increased demand in endemic and non-endemic countries with better structure of their national surveillance and healthcare systems (38) (294) (295) . Benznidazole (Class 1, level of evidence b) is produced as tablets of 100mg and 50mg (adults) and 12.5mg and 50mg (children) (20) (42) (313) . In Brazil, only the 100mg and 12.5mg tablets are available in the SUS network.…”

Section: Treatment Options Dosage and Type Of Administrationmentioning

confidence: 99%

“…PCR is an alternative option to indirect methods (xenodiagnosis and blood culture) in the parasitologic evaluation as a treatment failure criterion after completing antiparasitic treatment (104) (313) . However, there are still limitations on the availability and standardization of techniques in the health networks of endemic countries (313) .…”

Section: Evaluation Of Cure Of Chagas Disease After Antiparasitic Trementioning

confidence: 99%

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2 nd Brazilian Consensus on Chagas Disease, 2015

Dias

Ramos

Gontijo

et al. 2016

Rev. Soc. Bras. Med. Trop.

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286

485

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Section: Treatment Options Dosage and Type Of Administrationmentioning

confidence: 99%

Section: Evaluation Of Cure Of Chagas Disease After Antiparasitic Trementioning

confidence: 99%

2 nd Brazilian Consensus on Chagas Disease, 2015

Dias

Ramos

Gontijo

et al. 2016

Rev. Soc. Bras. Med. Trop.

Self Cite

286

485

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“…The molecular biology by means of the polymerase chain reaction technique, is already well studied in Chagas disese 101 , but has not been a frequently utilized method for indirect research of T. cruzi in the presence of the co-infection with HIV/AIDS. However, it was described in two (0.7%) cases used for diagnostic confirmation in peripheral blood 92 , and on the study of the parasite in duodenal biopsies in four co-infected individuals 51 .…”

Section: Casesmentioning

confidence: 99%

Co-infection Trypanosoma cruzi/HIV: systematic review (1980 - 2010)

Almeida

Ramos

Correia

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction:The co-infection Trypanosoma cruzi/HIV has been described as a clinical event of great relevance. The objective of this study was to describe clinical and epidemiological aspects published in literature. Methods: It is a systematic review of a descriptive nature from the databases Medline, Lilacs, SciELO, Scopus, from 1980 to 2010. Results: There were 83 articles (2.8 articles/year) with a total of 291 cases. The co-infection was described in 1980 and this situation has become the defining AIDS clinical event in Brazil. This is the country with the highest number of publication (51.8%) followed by Argentina (27.7%). The majority of cases are amongst adult men (65.3%) native or from endemic regions with serological diagnosis in the chronic stage (97.9%) and indeterminate form (50.8%). Both diseases follow the normal course, but in 41% the reactivation of the Chagas disease occurs. The most severe form is the meningoencephalitis, with 100% of mortality without specific and early treatment of the T. cruzi. The medication of choice was the benznidazole on doses and duration normally used for the acute phase. The high parasitemia detected by direct or indirect quantitative methods indicated reactivation and its elevation is the most important predictive factor. The lower survival rate was related to the reactivation of the Chagas disease and the natural complications of both diseases. The role of the antiretroviral treatment on the co-infection cannot yet be defined by the knowledge currently existent. Conclusions: Despite the relevance of this clinical event there are still gaps to be filled.

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“…On the other hand, molecular technology demonstrates the presence of parasites based on their antigenic components or DNA segments (3). These tests are not influenced by environmental factors that usually can interfere with the results of a stool test, for example, thus ensuring highly reliable results (4).…”

Section: Introductionmentioning

confidence: 99%

Molecular techniques for the study and diagnosis of parasite infection

Tavares¹,

Staggemeier²,

Borges³

et al. 2011

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition to microsatellites. Molecular assays have comprehensively assisted in the diagnosis, treatment and epidemiological studies of parasitic diseases that affect people worldwide, helping to control parasitic disease mortality.Key words: parasite infection, diagnosis, molecular techniques, molecular epidemiology. Review ARticle The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2011 | volume 17 | issue 3 | pages 239-248 INTRODUCTIONIn parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites (1). However, the occasional difficulty of identifying these parasite structures may decrease the sensitivity of such methods. Currently, because of these difficulties, molecular biology has been employed to detect parasites responsible for parasitic diseases (2).Traditional parasitological analyses have the advantage of being less costly without requiring expensive reagents and equipment. Additionally, such analyses can be easily performed when a trained microscopist is available. On the other hand, molecular technology demonstrates the presence of parasites based on their antigenic components or DNA segments (3). These tests are not influenced by environmental factors that usually can interfere with the results of a stool test, for example, thus ensuring highly reliable results (4).Current laboratory diagnostic methods for the identification of parasites include: polymerase chain reaction (PCR), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), microsatellite marker method, Luminex xMAP-based technology (areas of multianalyte profiling), loop-mediated isothermal amplification (LAMP), and the recently added real-time PCR (5-12).The objective of the present article was to review the applications of molecular biology techniques in parasitology by evaluating and discussing their uses and benefits. MOLECULAR METHODS FOR DETECTION OF PARASITE STRUCTURESSeveral molecular tests to detect parasites have been developed in the last decade. Their specificity and sensitivity have gradually increased, and parasites that were previously difficult to diagnose usi...

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Doença de Chagas crônica: do xenodiagnóstico e hemocultura à reação em cadeia da polimerase

Cited by 68 publications

References 45 publications

2 nd Brazilian Consensus on Chagas Disease, 2015

2 nd Brazilian Consensus on Chagas Disease, 2015

Co-infection Trypanosoma cruzi/HIV: systematic review (1980 - 2010)

Molecular techniques for the study and diagnosis of parasite infection

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