DODİN FUNGUSİTİNİN Vicia faba L. BİTKİSİNDE TOTAL PROTEİN MİKTARI VE PEROKSİDAZ AKTİVİTESİ ÜZERİNE ETKİSİ

Çördük, Nurşen; Akıncı, Nihan; Kaya, Nergis; Yücel, Gülru; Akı, Cüneyt

doi:10.16984/saufenbilder.22241

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…Pesticides can be grouped into different classes based on the chemical type, target organisms, mechanism of action and type of application. According to their chemical types, they are categorized into four main groups known as organochlorine pesticides, carbamate pesticides, pyrethroid pesticides and organophosphate pesticides [5][6][7]. Among the pesticides used for a variety of purposes, such as the protection of crops, fields, and trees, and the control of mosquitoes and other pests, pyrethroids insecticides are synthetic analogs of pyrethrin.…”

Section: Introductionmentioning

confidence: 99%

Determination of Resmethrin in Corn Silk Matrix by Gas Chromatography-Flame Ionization Detector (GC-FID)

Serbest¹

2022

Sakarya University Journal of Science

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The use of pesticides provides benefits such as growing food products necessary for life, sustainability of production efficiency and prevention of diseases caused by pests. However, these benefits can turn into negative effects for humans and other living organisms with the use of pesticides in inappropriate doses and methods. In this study, it was aimed to determine resmethrin, which belongs to the insecticide class, by gas chromatography-flame ionization detector (GC-FID) method. Hence, a suitable temperature program was utilized to separate resmethrin at a certain retention time and satisfactory system analytical performance values were recorded under the proposed conditions. After corn silk sample was extracted using acetonitrile, analytical measurements were carried out for prepared sample to investigate the presence of resmethrin. There was no analytical signal belongs to resmethrin in blank analysis. Recovery experiments were performed using external calibration method with spiked samples at three concentrations and good percent recovery results were obtained between 93.6 – 96.9%.

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Section: Introductionmentioning

confidence: 99%

Determination of Resmethrin in Corn Silk Matrix by Gas Chromatography-Flame Ionization Detector (GC-FID)

Serbest¹

2022

Sakarya University Journal of Science

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Evaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis Cinerea) in Vicia Faba Leaves

Kaya

Yiğit

2023

Sakarya University Journal of Science

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The antioxidant defense system of the medicinal plant Raphanus sativus L. tuber root in terms of certain antioxidant enzymes (ascorbat peroxidase, glutathion reductase, catalase, superoxide dismutase) and lipid peroxidation against gray mold disease caused by Botrytis cinerea fungus on Vicia faba L. (bean) leaves was analyzed spectrophotometrically. V. faba plants were grown from seed under controlled conditions. A spore suspension of 105 spores/ml of B. cinerea was prepared. The leaves of V. faba were treated with distilled water, only DMSO, only B. cinerea (positive control), only extract applications (distilled water, ethanol and methanol extract) and B. cinerea treatment after extract applications. The leaves of V. faba were harvested 24 and 48 hours after the applications. Analyzes of the supernatants obtained from the extracts were performed spectrophotometrically. It has been revealed that the activity of all investigated enzymes generally increased in the extract:fungus application groups compared to the fungus application group. It was determined that the application of fungus alone caused an increase in the activity of enzymes compared to the application groups other than the extract:fungus application groups. It was observed that there was an increase in lipid peroxidation (TBARS) only in the fungus application group compared to all extract:fungus application groups except 10mg/ml distilled water extract:fungus treatment. As a result of the research, it was determined that the extract:fungus applications generally increased the antioxidant enzyme activity levels and decreased the TBARS content when compared to the negative control and positive control groups. In extract:fungus applications, enzyme activities were obtained from 10mg/ml distilled water extract:fungus, 10mg/ml EtOH extract:fungus, 10mg/ml MeOH extract:fungus applications. From this point of view, it can be stated that R. sativus distilled water, ethanol and methanol extracts generally give an increased antioxidant defense response in V. faba leaves compared to B. cinerea inoculation alone.

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In vitro effect of four fungicides on Colletotrichum gloeosporioides causing anthracnosis on the Red Globe grape variety

López-Zapata

Castaño-Zapata

2020

Rev. Acad. Colomb. Cienc. Ex. Fis. Nat.

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Anthracnose is considered one of the most important diseases of grape crops. In this study, we evaluated the in vitro effect on potato dextrose agar (PDA) of four fungicides: benomyl (Benlate® 50 WP), carbendazim (Belico ® 500 SC), chlorothalonil (Odeon® 720 SC), and dodine (Syllit ® 400 SC) using three concentrations on a Colletotrichum gloeosporioides isolate. This species complex causes anthracnose on the Red Globe grape variety. The concentrations of each fungicide were: benomyl at 0.5, 0.25, and 1 g.L-1; carbendazim at 0.6, 0.3, and 1.2 mL.L-1; chlorothalonil at 2.4, 1.2, and 4.8 mL.L-1, and dodine at 1.6, 0.8, and 3.2 mL.L-1. Evaluated the inhibition of mycelial growth and the sporulation of the fungus. The fungus mycelium exposed to the commercial dose of the fungicides was observed using environmental scanning electron microscopy (ESEM). We used a completely randomized design with 13 treatments and five repetitions. Our results showed a degree of differential efficacy for the four fungicides and their effects were directly proportional to the dose used, benomyl and chlorothalonil being the most efficient in controlling the mycelial growth of the fungus. At the commercial dose, sporulation was inhibited at a higher rate by benomyl showing no statistically significant differences with the other products except chlorothalonil and dodine when the recommended dose was reduced by half. The damage observed by ESEM on the fungus mycelium ratified the effect obtained in vitro, i.e., deformed hyphae and irregular growth. The results of this study, complemented by electron microscopy, are promising for the appropriate selection of the best fungicides for controlling anthracnose on grape fruits, subject to the timely and correct diagnosis of the disease.

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DODİN FUNGUSİTİNİN Vicia faba L. BİTKİSİNDE TOTAL PROTEİN MİKTARI VE PEROKSİDAZ AKTİVİTESİ ÜZERİNE ETKİSİ

Cited by 3 publications

References 35 publications

Determination of Resmethrin in Corn Silk Matrix by Gas Chromatography-Flame Ionization Detector (GC-FID)

Determination of Resmethrin in Corn Silk Matrix by Gas Chromatography-Flame Ionization Detector (GC-FID)

Evaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis Cinerea) in Vicia Faba Leaves

In vitro effect of four fungicides on Colletotrichum gloeosporioides causing anthracnosis on the Red Globe grape variety

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