Evaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis Cinerea) in Vicia Faba Leaves

Abstract

The antioxidant defense system of the medicinal plant Raphanus sativus L. tuber root in terms of certain antioxidant enzymes (ascorbat peroxidase, glutathion reductase, catalase, superoxide dismutase) and lipid peroxidation against gray mold disease caused by Botrytis cinerea fungus on Vicia faba L. (bean) leaves was analyzed spectrophotometrically. V. faba plants were grown from seed under controlled conditions. A spore suspension of 105 spores/ml of B. cinerea was prepared. The leaves of V. faba were treated with distilled water, only DMSO, only B. cinerea (positive control), only extract applications (distilled water, ethanol and methanol extract) and B. cinerea treatment after extract applications. The leaves of V. faba were harvested 24 and 48 hours after the applications. Analyzes of the supernatants obtained from the extracts were performed spectrophotometrically. It has been revealed that the activity of all investigated enzymes generally increased in the extract:fungus application groups compared to the fungus application group. It was determined that the application of fungus alone caused an increase in the activity of enzymes compared to the application groups other than the extract:fungus application groups. It was observed that there was an increase in lipid peroxidation (TBARS) only in the fungus application group compared to all extract:fungus application groups except 10mg/ml distilled water extract:fungus treatment. As a result of the research, it was determined that the extract:fungus applications generally increased the antioxidant enzyme activity levels and decreased the TBARS content when compared to the negative control and positive control groups. In extract:fungus applications, enzyme activities were obtained from 10mg/ml distilled water extract:fungus, 10mg/ml EtOH extract:fungus, 10mg/ml MeOH extract:fungus applications. From this point of view, it can be stated that R. sativus distilled water, ethanol and methanol extracts generally give an increased antioxidant defense response in V. faba leaves compared to B. cinerea inoculation alone.