Docosahexaenoic acid and other dietary polyunsaturated fatty acids suppress leukotriene synthesis by mouse peritoneal macrophages

Lokesh, Belur R.; Black, J. Mark; German, J. Bruce; Kinsella, John E.

doi:10.1007/bf02536345

Cited by 69 publications

(19 citation statements)

References 45 publications

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“…The effects of DHA administration on in vitro ( 16,18,20) or ex vivo ( 17,19) LTB4 production have been discussed in several studies. However, the methods of administration of DHA in these studies were either oral or in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Effects of DHA administration on LTB4 synthesis were discussed in several studies (16)(17)(18)(19)(20). Lokesh et al ( 19) reported that the LTB4 and LTC4 production by peritoneal macrophages of mice was reduced by dietary supplementation of a DHA-rich fish oil.…”

Section: Introductionmentioning

confidence: 99%

“…Lokesh et al ( 19) reported that the LTB4 and LTC4 production by peritoneal macrophages of mice was reduced by dietary supplementation of a DHA-rich fish oil. However, a period as long as 10 d of oral administration of DHA might be necessary to reduce LTB4 production ( 19). The effect of this type of intervention is not rapid enough for management of acute diseases related to LTB4.…”

Section: Introductionmentioning

confidence: 99%

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Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits. Effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes.

Nakamura¹,

Hamazaki²,

Yamazaki³

et al. 1993

J. Clin. Invest.

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Leukotriene (LT) B4 is a major chemical activator of PMN. Inhibitory effects of oral administration of docosahexaenoic acid (DHA) on LTB4 synthesis by PMN are known. We intravenously infused tridocosahexaenoyl-glycerol (DHA-TG) emulsion into rabbits in three different doses, namely 0.8, 0.4, or 0.2 g DHA/kg, and investigated the changes in LTB4/1 production by ionophore-activated PMN. The averaged LTB4 production by PMN was significantly reduced to 57 and 59% of baseline at 6 h after the infusion of 0.8 and 0.4 g DHA/ kg, respectively (P < 0.05), but not after the infusion of 0.2 g DHA/kg or 0.8 g soybean oil/kg. The combined concentrations of both DHA and eicosapentaenoic acid in the PMN phospholipid fraction were significantly increased at 6 h after the infusion of 0.8 or 0.4 g DHA / kg but not after the infusion of0.2 g DHA / kg or 0.8 g soybean oil / kg. Oral administration of 0.8 g DHA/kg did not increase DHA or eicosapentaenoic acid in the PMN phospholipid fraction and did not decrease LTB4

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits. Effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes.

Nakamura¹,

Hamazaki²,

Yamazaki³

et al. 1993

J. Clin. Invest.

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“…Yet, the data on dietinduced changes of their PUFA content are limited to three studies with human neutrophils, one study comparing five different rat strains, and a possibly a single mouse study (Table 2). There is some uncertainty about the identification of the immune cells analyzed by Lokesh et al [37] These researchers indicated that they were working with macrophages, yet the cells isolated were from the peritoneum of mice 30 min following the injection of an inflammatory agent (i.e., zymosan). The predominate immune cell that would have been recovered under these circumstances would have been neutrophils, not macrophages.…”

Section: Neutrophilsmentioning

confidence: 97%

Important Differences Exist in the Dose–Response Relationship between Diet and Immune Cell Fatty Acids in Humans and Rodents

Fritsche

2007

Lipids

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Omega-3 polyunsaturated fatty acids (n-3 PUFA) are noted for their ability to diminish inflammatory and immune responses in vitro and in a variety of animal-based models of autoimmunity and inflammation. Yet, recent systematic reviews suggest that the evidence for these fatty acids having beneficial effects on inflammation or autoimmunity in humans is equivocal. A possible explanation for these disappointing and somewhat paradoxical findings emerged from the analyses described in this review. The available data on the changes in immune cell fatty acid profiles in mice, rats and humans, fed various forms and amounts of n-3 PUFA are summarized and displayed graphically. The dose-response curves generated provide new insights into the relationship between dietary n-3 PUFA and immune cell fatty acid profiles. The author suggests that the poor predictive value of most in vitro as well as many animal trials may, in part, be a consequence of the frequent adoption of experimental conditions that create differences in immune cell fatty acid profiles that far exceed what is possible in free-living humans through dietary intervention. Recommendations for improving the preclinical value of future in vitro and animal-based studies with n-3 PUFA are provided.

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“…A study by Danno et al [27] reported that the mouse ear edema reaction induced by AA and UVB irradiation was decreased by EPA but not by DHA. Lokesh et al [28,29], found that DHA was as effec tive as EPA in suppressing eicosanoid synthesis in mice pe ritoneal macrophages. DHA ethyl ester was more effective than EPA ethyl ester in alleviating murine autoimmune glo merulonephritis, and the effects of the two fatty acids may be synergistic [30].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Properties of Docosahexaenoic and Eicosapentaenoic Acids in Phorbol-Ester-lnduced Mouse Ear Inflammation

Raederstorff¹,

Pantze²,

Bachmann³

et al. 1996

Int Arch Allergy Immunol

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Laboratory animal models and clinical studies suggest that dietary n-3 fatty acids are beneficial in diseases with an inflammatory component such as rheumatoid arthritis or psoriasis. In the present study we investigated the effect of purified docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on phorbol ester (TPA)-induced acute inflammation. Mice were fed for 6 weeks a diet containing 5% corn oil enriched with either 1% DHA or 1% EPA and compared with a group receiving 6% corn oil only. The dietary treatment with DHA or EPA elevated the n-3 polyunsaturated fatty acids as expected in the spleen and ear phospholipids, associated with a reduction in arachidonic acid levels. The degree of ear inflammation was quantified by measuring the four parameters including (1) edema as the increase in ear biopsy weight, (2) polymorphonuclear cell infiltration as myeloperoxidase activity (MPO) at the site of inflammation, (3) prostaglandin E₂ (PGE₂) and (4) leukotriene B₄ (LTB₄) concentrations in ear edema. The addition of DHA to the diet reduced significantly the edema formation and the MPO activity 24 h after a TPA challenge. Both DHA and EPA significantly reduced the PGE₂ and LTB₄ levels compared with animals fed corn oil. This result suggests that DHA rather than EPA may be useful in the adjuvant treatment of diseases where acute inflammatory processes play a role.

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Docosahexaenoic acid and other dietary polyunsaturated fatty acids suppress leukotriene synthesis by mouse peritoneal macrophages

Cited by 69 publications

References 45 publications

Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits. Effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes.

Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits. Effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes.

Important Differences Exist in the Dose–Response Relationship between Diet and Immune Cell Fatty Acids in Humans and Rodents

Anti-Inflammatory Properties of Docosahexaenoic and Eicosapentaenoic Acids in Phorbol-Ester-lnduced Mouse Ear Inflammation

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