The reason why cooperation occurs in repeated games has remained a puzzle. Earlier literature has maintained that reciprocal behavior that gives rise to cooperation can be entirely self-regarding. However, experimental evidence shows that reciprocal behavior is otherregarding in many one-shot games. This other-regarding behavior is believed to have an emotional foundation. We hypothesized that emotions play a role in reciprocal behavior in repeated games as well. We tested this hypothesis by measuring the psychophysiological correlates of emotions from pairs of subjects as they played a repeated Cournot duopoly game.The players, who were in different rooms and remained anonymous to each other, made adjustment decisions to their production quantities that determined their payoffs in each round.Autonomic nervous system arousal was activated when the payoffs of both players decreased in a round, whereas positive affect was expressed when the payoffs of both players increased in a round. The disgust expression was related to a player's own one-sided increase in the payoff.Anger was expressed occasionally but less frequently when the outcome was the player's ideal outcome. An upwards adjustment of the production quantity was observed when the other player did not cooperate. This had the effect of decreasing the payoffs of both players and this was also related to an increase in the level of arousal. Our results provide evidence on how emotions are present in reciprocal behavior in a repeated social dilemma game. The results challenge recent behavioral research that advocates self-regarded motivations of cooperation in repeated games. What is the role of emotions in cooperation in repeated games? Mounting experimental evidence from one-shot games shows that cooperative behavior is related to emotions (Camerer and Fehr, 2004;Camerer et al., 2005;Fessler and Haley, 2003;Haidt, 2007;Loewenstein et al., 2008;Phelps, 2009;Sanfey, 2007) despite of the fact that in one-shot games the players interact anonymously and expect to never meet again in the same situation. Research in behavioral economics has explained cooperation in one-shot games by the players' preferences for equity and fairness and the reciprocal punishment and reward strategies that arise from these preferences (Boyd et al., 2010;Fehr and Fischbacher, 2002;Sobel, 2005). The concepts of emotions and other-regarding preferences are two sides of the same coin. For example, unfair offers in the Ultimatum Game elicit the negative emotions of anger (Pillutla and Murnighan, 1996), sadness (Harlé and Sanfey, 2007), and disgust (Chapman et al., 2009).The findings of emotional activity in cooperation in one-shot games cannot be generalized to repeated games. This is because of the Folk Theorem arguments that are most often used to explain behavior in repeated interaction. According to the Folk Theorem, reciprocal punishments and rewards that typically demonstrate other-regarding behavior in one-shot games can be part of an adaptive and rational response strategy...