Do leaf total antioxidant capacities (TAC) reflect specific antioxidant potentials? – A comparison of TAC and reactive oxygen scavenging in tobacco leaf extracts

Majer, Petra; Stoyanova, Silviya; Hideg, Éva

doi:10.1016/j.jphotobiol.2010.04.007

Cited by 10 publications

(16 citation statements)

References 28 publications

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“…7C). This is different from what we found earlier in senescing tobacco leaves, where FRAP and total phenolics were strongly correlated and TEAC appeared less related to the two other parameters [24]. This difference between results of the two experiments which are only common in changes of antioxidant levels is not surprising.…”

Section: Discussioncontrasting

confidence: 99%

“…On the other hand, the lack of a linear correlation between FRAP and either hydroxyl radical or singlet oxygen scavenging, which was also found earlier [24] confirms that these specific antioxidant capacities are not decisive main constituents of FRAP.…”

Section: Discussionsupporting

confidence: 77%

“…Currently available ROS detecting methods are, however, not sensitive enough to identify ROS directly under milder stress conditions, leaving only the possibility of assuming that increased antioxidant activities reflect a situation requiring stronger control over the increased level of ROS. In addition to measuring ROS specific antioxidant enzymes, plant studies have also borrowed methods from medicine and food chemistry to assess total antioxidant potentials of a plant extracts [22][23][24][25]. Comparisons of these general and ROS specific antioxidant capacities are of interest.…”

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confidence: 99%

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Developmental stage is an important factor that determines the antioxidant responses of young and old grapevine leaves under UV irradiation in a green-house

Majer

Hideg

2012

Plant Physiology and Biochemistry

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 77%

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confidence: 99%

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Developmental stage is an important factor that determines the antioxidant responses of young and old grapevine leaves under UV irradiation in a green-house

Majer

Hideg

2012

Plant Physiology and Biochemistry

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“…The TAC of leaves is frequently included in the set of parameters used to characterize plant stress responses (Majer et al, 2010). In the present study, leaf TAC increased in both UV-B-treated groups, indicating that the tobacco antioxidant system was stimulated by UV-B radiation.…”

Section: Discussionsupporting

confidence: 55%

“…The homogenized samples were centrifuged at 40,000 g for 25 min at 4°C, after which the supernatants were used for measurements. The principle of the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay is the scavenging mechanism of ABTS + radical by hydrogen-donating antioxidants in the sample, forming colorless ABTS (Majer et al, 2010). To form cation radicals, we mixed 0.1 mM ABTS, 0.0125 µM horseradish peroxidase, and 1 mM H 2 O 2 with 50 mM phosphate buffer, pH 6.…”

Section: Trolox Equivalent Antioxidant Capacity (Teac) Assaymentioning

confidence: 99%

Effect of increased UV-B radiation on carotenoid accumulation and total antioxidant capacity in tobacco (Nicotiana tabacum L.) leaves

et al. 2017

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ABSTRACT.Carotenoids are important components of plant antioxidant systems, which protect photosystems from photooxidative destruction during ultraviolet-B (UV-B) exposure. The influence of carotenoids on total antioxidant capacity (TAC) of plants has rarely been studied. In this study, tobacco (Nicotiana tabacum L., 'K326') seedlings exposed to UV-B radiation were used in order to evaluate the effects of ambient levels of UV-B radiation on carotenoid accumulation. The aim was to investigate whether carotenoids could enhance TAC as a means of UV protection. Our results showed that leaf carotenoid content in the low UV-B exposure (+9.75 mW/cm 2 ) plants was approximately 8% higher than that observed in control plants at 2-8 days of exposure. At high UV-B exposure (+20.76 mW/cm 2 ), the carotenoid content increased rapidly after 1 day's exposure (10.41% higher than the control), followed by a return to the content as in control plants. Furthermore, carotenoid content positively correlated with TAC (P = 0.024). These results suggest that carotenoids have antioxidant properties and play an important role in the antioxidant system. UV-B exposure increased the carotenoid synthesis capability of plants. The plants could deplete the carotenoids to scavenge excess ROS at high UV-B radiation levels, which protects the tobacco plant from oxidative damage caused by UV-B stress.

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Overexpression of homogentisate phytyltransferase (HPT) and tocopherol cyclase (TC) enhances α-tocopherol content in transgenic tobacco

Harish¹,

Dachinamoorthy²,

Balamurugan³

et al. 2013

Biol. plant.

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Photosynthetic organisms synthesize the amphipathic antioxidants called tocopherols which are essential components of the human diet. To increase the α-tocopherol (vitamin E) content, Arabidopsis genes encoding homogentisate phytyltransferase (HPT) and tocopherol cyclase (TC) were constitutively expressed individually and in combination (HPT:TC) in tobacco plant by Agrobacterium mediated transformation. The transgene was confirmed by polymerase chain reaction (PCR), transgene expression was studied by reverse transcriptase (RT)-PCR, integration of the transgene in the plant genome was confirmed by Southern blot, and α-tocopherol content was quantified using high performance liquid chromatography (HPLC). The α-tocopherol content in transgenic tobacco plants expressing HPT, TC, and HPT:TC was increased by 5.4-, 4.0-, and 7.1-fold, respectively, when compared to the wild type (WT). These results indicate that, the HPT and TC activities are critical for enhancing the vitamin E content in tobacco plants.

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Do leaf total antioxidant capacities (TAC) reflect specific antioxidant potentials? – A comparison of TAC and reactive oxygen scavenging in tobacco leaf extracts

Cited by 10 publications

References 28 publications

Developmental stage is an important factor that determines the antioxidant responses of young and old grapevine leaves under UV irradiation in a green-house

Developmental stage is an important factor that determines the antioxidant responses of young and old grapevine leaves under UV irradiation in a green-house

Effect of increased UV-B radiation on carotenoid accumulation and total antioxidant capacity in tobacco (Nicotiana tabacum L.) leaves

Overexpression of homogentisate phytyltransferase (HPT) and tocopherol cyclase (TC) enhances α-tocopherol content in transgenic tobacco

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