Do ectothermic vertebrates have a home in which to affinity mature their antibody responses?

Muthupandian, Aradana; Waly, Doaa; Magor, Brad G.

doi:10.1016/j.dci.2021.104021

Cited by 11 publications

(7 citation statements)

References 74 publications

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“…This, in turn, may result in a weaker selection of high-affinity clones. Magor (Magor 2015;Muthupandian et al 2021) suggests that this could explain the slow and weak affinity maturation found in fish. The main increase of specific ASC in the PerC observed in our study suggests that clonal selection might be possible locally.…”

Section: Non-specific Versus Specific B Cell Activationmentioning

confidence: 99%

“…Nevertheless, the location and mechanisms of the teleost affinity maturation are still largely unknown (Magor 2015), and the affinity maturation in fishes is generally considered as much less efficient than in mammals (Kaattari 2002). The absence of true germinal centers is often provided as an explanation, resulting in an inefficient selection of highaffinity clones (Magor 2015;Muthupandian et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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The importance of the Atlantic salmon peritoneal cavity B cell response: Local IgM secreting cells are predominant upon Piscirickettsia salmonis infection

Wal¹,

Jenberie²,

Nordli³

et al. 2021

Developmental & Comparative Immunology

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Section: Non-specific Versus Specific B Cell Activationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The importance of the Atlantic salmon peritoneal cavity B cell response: Local IgM secreting cells are predominant upon Piscirickettsia salmonis infection

Wal¹,

Jenberie²,

Nordli³

et al. 2021

Developmental & Comparative Immunology

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“…Further studies would have to determine the nature of these concentrations of lymphoid cells we observed in NELO and if they represent structures favoring processes of adaptive immunity such as Ag presentation. It would be particularly interesting to determine whether they are associated with the formation of melanomacrophage centers, which are immune structures that have been suggested as potential analogs of mammalian germinal centers in teleost fish ( 82 ) and are occasionally observed in NELO of Atlantic salmon (fig. S12, cyan arrowheads) and zebrafish (fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?

Resseguier,

Nguyen-Chi,

Wohlmann

et al. 2023

Sci. Adv.

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The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses to be extremely important for their survival. Here, we used a marker for T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques to investigate the lymphoid architecture of the zebrafish branchial cavity. We identified a sub-pharyngeal lymphoid organ, which we tentatively named “Nemausean lymphoid organ” (NELO). NELO is enriched in T/NK cells, plasma/B cells, and antigen-presenting cells embedded in a network of reticulated epithelial cells. The presence of activated T cells and lymphocyte proliferation, but not V(D)J recombination or hematopoiesis, suggests that NELO is a secondary lymphoid organ. In response to infection, NELO displays structural changes including the formation of T/NK cell clusters. NELO and gill lymphoid tissues form a cohesive unit within a large mucosal lymphoid network. Collectively, we reveal an unreported mucosal lymphoid organ reminiscent of mammalian tonsils that evolved in multiple teleost fish families.

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“…Further studies would have to determine the nature of these concentrations of lymphoid cells we observed in NEMO and if they represent structures favoring processes of adaptive immunity such as antigen-presentation. It would be particularly interesting to determine if they are associated to the formation of melanomacrophage center (MMC), which are immune structure that have been suggested as potential fish analogue of mammalian germinal center ( 106 ). In the present study and our previous one on the zebrafish GIALT ( 36 ), we showed that in contrast to NEMO, upon viral infection with SVCV or IHNV, the zebrafish ILT and ALT are first strongly reduced by 3 dpi before being replenished a week later.…”

Section: Discussionmentioning

confidence: 99%

Identification of a new pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: tonsils in fish?

Rességuier

NGUYEN-CHI

Wohlmann

et al. 2023

Preprint

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The evolution of fish in an aqueous environment, with the large gills surface continuously exposed to pathogens, must have driven local mucosal immune responses to be extremely important for survival. In this study, we used a T/NK cells marker, ZAP70, and advanced imaging techniques to investigate the lymphoid architecture of the branchial cavity in adult zebrafish. With this approach, we identified a new lymphoid organ (NEMO), within the sub-pharyngeal region and in close association with gills. Besides T/NK cells, NEMO is also enriched in plasma/B cells and antigen-presenting cells that are embedded in a network of reticulated epithelial cells. While this organ is not associated with V(D)J recombination or hematopoiesis, it exhibits features of secondary lymphoid organs, such as the presence of activated T cells and lymphocyte proliferation. In response to viral and parasitic infections, NEMO displays structural changes that are typically associated with immune responses to pathogens. NEMO is part of a large lymphoid network that interconnects all the immune structures within the branchial cavity. Importantly, NEMO could clearly be identified in multiple teleost fish families. Our findings reveal a new mucosal lymphoid organ that is reminiscent of mammalian tonsils.

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Do ectothermic vertebrates have a home in which to affinity mature their antibody responses?

Cited by 11 publications

References 74 publications

The importance of the Atlantic salmon peritoneal cavity B cell response: Local IgM secreting cells are predominant upon Piscirickettsia salmonis infection

The importance of the Atlantic salmon peritoneal cavity B cell response: Local IgM secreting cells are predominant upon Piscirickettsia salmonis infection

Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?

Identification of a new pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: tonsils in fish?

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