Do collagen cross-linkers improve dentin’s bonding receptiveness?

Gré, C.P.; Lise, Diogo Pedrollo; Ayres, Ana Paula Almeida; Munck, Jan De; Tezvergil‐Mutluay, Arzu; Seseogullari‐Dirihan, Roda; Lopes, Guilherme Carpena; Landuyt, Kirsten Van; Meerbeek, Bart Van

doi:10.1016/j.dental.2018.08.303

Cited by 24 publications

(13 citation statements)

References 49 publications

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“…First, the remnant water in demineralized dentin has resulted in a highly porous hybrid layer [ 6 ]. The interspaces at the bottom of the hybrid layer cannot be completely occupied by adhesive monomers, and the denuded demineralized collagen fibrils are therefore under great threat from water hydrolysis and protease degradation [ 7 , 8 ]. Second, the ester group (–CO–O-R-) in both acrylate- and methacrylate-based monomers, especially 2-hydroxyethyl methacrylate (HEMA), could be hydrolyzed by water over time, which is detrimental to the mechanical properties of the hybrid layer [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancing resin-dentin bond durability using a novel mussel-inspired monomer

Yao

Ying

et al. 2021

Materials Today Bio

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Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N -(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a functional monomer in dental adhesive, its potential property to cross-link with dentin collagen and polymerize with adhesive will first be evaluated by transmission electron microscopy (TEM), attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR), and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution as a primer in dentin bonding was examined using micro-tensile bond strength (μTBS) testing, fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. The inhibitory effect of DMA on endogenous metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8. Results demonstrated that DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. Furthermore, even after 10,000 times thermocycling aging, the μTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values. In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 μmol/L. Thus, DMA could be used as a novel, biocompatible functional monomer in dentin bonding.

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Section: Introductionmentioning

confidence: 99%

Enhancing resin-dentin bond durability using a novel mussel-inspired monomer

Yao

Ying

et al. 2021

Materials Today Bio

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“…In this manner, these agents are regarded to increase the bond strength of bonded restorations to dentin, to reduce the biodegradation rate of demineralized collagen, and consequently, to increase the longevity of adhesive restorations [4,26]. Cross-linking agents are claimed to react in different ways with collagen, creating different kinds and extents of cross-links, mainly via bonding with hydrogen ions (with natural agents) and covalent bonds (with synthetic agents) [6,27]. Plant extracts have also been used to induce dentin biomodification mainly due to the presence of tannins, especially proanthocyanidins [28].…”

Section: Discussionmentioning

confidence: 99%

“…Tannin-rich extracts as cross-linkers prior to the application of adhesive restorative materials demonstrated superior results when compared with chlorhexidine [33][34][35][36]. Conversely, concerns have been expressed over their actual benefits, especially when cross-linkers are applied in clinically relevant application times [27]. It has been questioned that the ability of a cross-linking agent to react with the dentin matrix to avoid the degradation of collagen at the adhesive interfacial area depends on its chemical and structural characteristics [27].…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, concerns have been expressed over their actual benefits, especially when cross-linkers are applied in clinically relevant application times [27]. It has been questioned that the ability of a cross-linking agent to react with the dentin matrix to avoid the degradation of collagen at the adhesive interfacial area depends on its chemical and structural characteristics [27]. In the present study, only TNA proved to be equally effective in chemically modifying collagen, which explains the reason to use it as a positive control.…”

Section: Discussionmentioning

confidence: 99%

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Influence of Dentin Priming with Tannin-Rich Plant Extracts on the Longevity of Bonded Composite Restorations

Polassi

Oliveira

Carvalho

et al. 2021

The Scientific World Journal

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Objective. This in vitro study evaluated the influence of bioactive plant extracts as dentin biomodifying agents to improve the longevity of bonded restorations. For that, plant extracts were applied to the dentin surface prior to the adhesive system. Materials and Methods. Bovine incisors were ground flat to obtain 2 mm thick slices in which conical preparations were made (N = 10). Tannin-containing plant extracts were applied to dentin before the application of the restorative system, as follows: control group (untreated, CTL), chlorhexidine 0.12% (CHX), mastruz (Dysphania ambrosioides, MTZ), cat’s claw (Uncaria tomentosa, CTC), guarana (Paullinia cupana, GUA), galla chinensis (Rhus chinensis, GCH), and tannic acid (extracted from Acacia decurrens, TNA). The push-out bond strength test was conducted (0.5 mm/min). Dentin biomodification was assessed by the modulus of elasticity and mass change in bovine tooth sections (0.5 × 1.7 × 7.0 mm). The dentin staining after extract treatments of dentin slices was compared. The dentin surface wettability was also evaluated by means of the contact angles of the adhesive system with the dentin surface and compared with the untreated control group. Data were subjected to ANOVA/Tukey’s test (α = 0.05). Results. The bond strength of the restoratives to dentin was not significantly improved by the plant extracts, irrespective of the evaluation time ( p > 0.05 ). Except for TNA, the elastic modulus of demineralized dentin significantly reduced after treatment with the plant extracts ( p < 0.05 ). The dentin staining correlated with the tannin content of the extracts. The contact angle was significantly reduced when treated with CTC, GCH, and TNA. Conclusions. The tannin-containing extracts had a questionable effect on the longevity of bonded restorations. The dentin modulus was negatively affected by the extract treatments. Although some of the extracts changed the contact angle, which seems to improve the adhesive monomer permeation, the tannin-rich plant extract application prior to adhesive application was proven to be clinically unfeasible due to dentin staining.

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“…Different MMP inhibitors such as quaternary ammonium methacrylates [17,28], and benzalkonium chloride [12] have been used experimentally to increase the durability of the resin-dentin interface [29]. Collagen cross-linkers have also been used to enhance the mechanical properties of the demineralized collagen network as well as bond durability in coronal [11,[30][31][32][33] and radicular dentin [34]. Because cross-linking of the collagen matrix is a naturally-occurring phenomenon in dentin, scientists have resorted to the use of chemical substances with cross-linking properties to render the dentin collagen matrix less susceptible to proteolytic attack [4,35].…”

Section: Introductionmentioning

confidence: 99%

Effect of an ethanol cross-linker on universal adhesive

et al. 2020

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Objective. To evaluate the effects of N,N'-dicyclohexylcarbodiimide (DCC), an ethanol-based dentin cross-linker, on the immediate and long-term microtensile bond strength (TBS) and nanoleakage expression of a universal adhesive employed in self-etch mode (SE) or etchand-rinse mode (ER). The effect of DCC on the dentinal MMP activity was also investigated by means of in-situ zymography.Methods. Eighty freshly extracted human molars were sectioned to expose mid-coronal dentin surfaces. The teeth were assigned to one of the following groups, according to the dentin surface priming/adhesive approach: (G1): DCC pre-treatment and Scotchbond Universal (SBU) in ER mode; (G2): SBU in ER mode; (G3): DCC pretreatment and SBU in SE mode;(G4): SBU in SE mode. TBS test was performed immediately (T 0 ) or after 1-year aging (T 12 ) in artificial saliva. Ten additional teeth per group were prepared for nanoleakage evaluation (N = 5) and for in-situ zymography (N = 5).Results. Three-factor analysis of variance revealed significant difference for the variables DCC pretreatment, application mode and aging (p < 0.05) for both microtensile bond strength testing and in-situ zymography. Nanoleakage analysis revealed reduced marginal infiltration of DCC experimental groups both at T 0 and T 12 .

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Do collagen cross-linkers improve dentin’s bonding receptiveness?

Cited by 24 publications

References 49 publications

Enhancing resin-dentin bond durability using a novel mussel-inspired monomer

Enhancing resin-dentin bond durability using a novel mussel-inspired monomer

Influence of Dentin Priming with Tannin-Rich Plant Extracts on the Longevity of Bonded Composite Restorations

Effect of an ethanol cross-linker on universal adhesive

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