Do Cellulose Binding Domains Increase Substrate Accessibility?

Esteghlalian, Ali R.; Srivastava, Vinit; Gilkes, Neil R.; Kilburn, Douglas G.; Warren, R. A. J.; Saddler, John N.

doi:10.1007/978-1-4612-0217-2_49

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Cellulase Structure1

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Cited by 4 publications

(1 citation statement)

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“…Many studies have shown that removal of the CBD typically results in a decrease of about 50-80 % of the activity of fungal cellulases [58,59]. The biochemical role of a CBD is to keep the enzyme catalytic unit close to the substrate surface [60]. Table 1.…”

Section: Cellulase Structurementioning

confidence: 99%

Fungal Biodegradation of Agro-Industrial Waste

Soliman¹,

El-Zawahry²,

El-Mougith³

2013

Cellulose - Biomass Conversion

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Section: Cellulase Structurementioning

confidence: 99%

Fungal Biodegradation of Agro-Industrial Waste

Soliman¹,

El-Zawahry²,

El-Mougith³

2013

Cellulose - Biomass Conversion

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Aerobic Microbial Cellulase Systems

Wilson

2008

Biomass Recalcitrance

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Biomass accessibility analysis using electron tomography

Hinkle

Ciesielski

Gruchalla

et al. 2015

Biotechnol Biofuels

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BackgroundSubstrate accessibility to catalysts has been a dominant theme in theories of biomass deconstruction. However, current methods of quantifying accessibility do not elucidate mechanisms for increased accessibility due to changes in microstructure following pretreatment.ResultsWe introduce methods for characterization of surface accessibility based on fine-scale microstructure of the plant cell wall as revealed by 3D electron tomography. These methods comprise a general framework, enabling analysis of image-based cell wall architecture using a flexible model of accessibility. We analyze corn stover cell walls, both native and after undergoing dilute acid pretreatment with and without a steam explosion process, as well as AFEX pretreatment.ConclusionImage-based measures provide useful information about how much pretreatments are able to increase biomass surface accessibility to a wide range of catalyst sizes. We find a strong dependence on probe size when measuring surface accessibility, with a substantial decrease in biomass surface accessibility to probe sizes above 5–10 nm radius compared to smaller probes.

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Do Cellulose Binding Domains Increase Substrate Accessibility?

Cited by 4 publications

References 26 publications

Fungal Biodegradation of Agro-Industrial Waste

Fungal Biodegradation of Agro-Industrial Waste

Aerobic Microbial Cellulase Systems

Biomass accessibility analysis using electron tomography

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