Do catalase and glutathione peroxidase protect blood platelets from lipid peroxidation in multiple sclerosis?

Abstract

Purpose: Catalase (cat) and glutathione peroxidase (GSH-Px) activities and thiobarbituric acid reactive substances (TBARS) concentration in blood platelets were determined in patients with multiple sclerosis (MS). Methods: The study was carried out in a group of 36 patients, men and women, aged 21-50 years old. They were divided into groups dependently on the degree of motor disability and duration of the disease. The control group included 15 healthy individuals of similar age to the study group. The activity of catalase was estimated according to the method by Beers and Sizer. Glutathione peroxidase activity was determined by the method of Sedlak and Lindsay, modified by Little and O'Brien. The concentration of thiobarbituric acid reactive substances in blood platelets was carried out using the method by Placer et al. Results: We observed a lower level of TBARS concentration in platelets of MS patients than in control group with an enhanced activities of both antioxidative enzymes on the basis of disability degree and duration of the disease. Conclusions: Catalase and glutathione peroxidase protect blood platelets from lipid peroxidation process in multiple sclerosis patients and may play a role in the course of the disease. It may also suggest involvement of lipid peroxidation in the activity of multiple sclerosis.

Contribution of Physical Activity to the Oxidative and Antioxidant Potential in 60–65-Year-Old Seniors

“…Hydrogen peroxide, the precursor of which is a superoxide anion radical, is an intraplatelet mediator of signaling pathways related to the stimulation of platelet aggregation by collagen and thrombin [ 74 , 75 ]. It is consistent with an observation that the degree of oxidative damage to platelet lipids is directly related to the activity of antioxidant enzymes, including hydrogen peroxide-scavenging catalase: the higher the catalase activity, the lower the degree of lipid peroxidation [ 76 ]. The role of hydrogen peroxide in shaping platelet reactivity is not simple and unambiguous.…”