Do Ca2+-chelating polysaccharides reduce calcium ion release from gypsum-based biomaterials?

Belcarz, Anna; Janczarek, Monika; Kolacz, Karolina; Urbanik‐Sypniewska, Teresa; Ginalska, Grażyna

doi:10.2478/s11535-013-0191-4

Cited by 3 publications

(4 citation statements)

References 36 publications

(44 reference statements)

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“…Following this hypothesis, such a deposition and pores clogging could probably reduce the surface area of HAp granules and slow down the rate of further uptake of Ca 2+ and PO 4 – ions. Release of Mg 2+ ions, parallel with the uptake of Ca 2+ and PO 4 – ions, is in agreement with our earlier results where such a specific Mg 2+ /Ca 2+ uptake/release equilibrium was observed for HAp/gypsum composite . One should remember, however, that the phenomenon of ions absorption‐desorption from the composite was observed for five days only.…”

Section: Discussionsupporting

confidence: 93%

“…Perhaps traces of apatite were formed on the composite surface but they were deposited within micropores of HAp granules (approximately 100 nm in size, as reported elsewhere 14 ) and this could explain the decreasing rate of Ca 21 -ions, is in agreement with our earlier results where such a specific Mg 21 /Ca 21 uptake/release equilibrium was observed for HAp/gypsum composite. 30 One should remember, however, that the phenomenon of ions absorption-desorption from the composite was observed for five days only.…”

Section: Discussionmentioning

confidence: 99%

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Behavior of new hydroxyapatite/glucan composite in human serum

et al. 2018

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Biomaterials for bone tissue regeneration, including polymer-based composites, are typically evaluated in vitro prior to the clinical trials. However, such composites tested in vivo may behave different due to the specific body conditions. For example, some composites implanted into the tissue acidified due to transient postoperative inflammation may unexpectedly swell which delays the wound healing. Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial. However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed. Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes. Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques. Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite. Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Behavior of new hydroxyapatite/glucan composite in human serum

et al. 2018

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“…4-fold) the growth rate of S. aureus (for C + CSD in comparison with C biomaterial), which can be explained by the stimulatory effect of calcium ions released by CSD. Calcium ions have been reported to affect the growth and metabolism of all organisms, including different bacteria, in a concentration-dependent manner [34][35][36]. This effect was not observed for E. coli (the viability of bacteria for C and C + CSD samples was similar).…”

Section: Antibacterial Activitymentioning

confidence: 97%

Gypsum-Related Impact on Antibiotic-Loaded Composite Based on Highly Porous Hydroxyapatite—Advantages and Disadvantages

Zalewska,

Vivcharenko,

Belcarz

2023

IJMS

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Highly porous hydroxyapatite is sometimes considered toxic and useless as a biomaterial for bone tissue regeneration because of the high adsorption of calcium and phosphate ions from cell culture media. This negatively affects the osteoblast’s growth in such ion-deprived media and suggests “false cytotoxicity” of tested hydroxyapatite. In our recent study, we showed that a small addition of calcium sulfate dihydrate (CSD) may compensate for this adsorption without a negative effect on other properties of hydroxyapatite-based biomaterials. This study was designed to verify whether such CSD-supplemented biomaterials may serve as antibiotic carriers. FTIR, roughness, mechanical strength analysis, drug release, hemocompatibility, cytotoxicity against human osteoblasts, and antibacterial activity were evaluated to characterize tested biomaterials. The results showed that the addition of 1.75% gypsum and gentamicin caused short-term calcium ion compensation in media incubated with the composite. The combination of both additives also increased antibacterial activity against bacteria representative of bone infections without affecting osteoblast proliferation, hemocompatibility, and mechanical parameters. Thus, gypsum and antibiotic supplementation may provide advanced functionality for bone-regeneration materials based on hydroxyapatite of a high surface area and increasingly high Ca2+ sorption capacity.

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“…It has already been shown that the application of calciumchelating polysaccharides (alginates or rhizobial exopolysaccharides) as a supplement to gypsum-based composite does not provide a sufficient, long-term efficacy in the removal of the excess of gypsumreleased Ca 2+ ions [13]. However, another concept may be tested for this purpose: the use of calcium ion-adsorbing ceramics.…”

Section: Introductionmentioning

confidence: 99%