Behavior of new hydroxyapatite/glucan composite in human serum

Abstract

Biomaterials for bone tissue regeneration, including polymer-based composites, are typically evaluated in vitro prior to the clinical trials. However, such composites tested in vivo may behave different due to the specific body conditions. For example, some composites implanted into the tissue acidified due to transient postoperative inflammation may unexpectedly swell which delays the wound healing. Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β-glucan biomaterial. However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed. Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes. Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques. Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite. Therefore, this suggests the lack of volume change-related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2653-2664, 2018.

Highly Porous Fluorapatite/β-1,3-Glucan Composite for Bone Tissue Regeneration: Characterization and In-Vitro Assessment of Biomedical Potential

“…170%, 140% and 130% of dry weight, respectively, after 1 min of soaking ( Figure 5 ). This observation was similar to our earlier observations described elsewhere [ 43 ]. For FAP/glucan composite, soaking capacity was in all cases lower than for relevant HAP/glucan ones.…”

“…Magnesium ions were absorbed from SBF neither by HAP nor by FAP-based composites ( Figure 6 b, Table 2 ). It was surprising because calcium and magnesium ions are usually absorbed simultaneously from ions-rich media by calcium phosphate ceramics [ 43 ]. More surprising, some magnesium ions were released by FAP/glucan composite to SBF during the first and second medium exchange; afterwards, no further Mg 2+ release was observed ( Figure 6 b).…”

The Impact of Contaminating Poly (Methyl Methacrylate) (PMMA) Bone Cements on Their Compressive Strength

“…In the present day, the advance of science remains fast-paced, especially in disciplines associated with medicine and human life. The need to save human lives, relieve pain, reconstruct parts of the human body after injuries, and, finally, cure those suffering from various kinds of local or systemic ailments using traditional methods [ 1 ] are the flywheels of progress both in medicine itself and many other disciplines that are subsidiary to medicine in terms of their classification, but which share its goals, such as material engineering and, in particular, the development of biomaterials [ 2 , 3 , 4 ]. Some biomaterials used to aid patients are bone cements.…”

Calcium Orthophosphate (CaPO4) Containing Composites for Biomedical Applications: Formulations, Properties, and Applications

“…Various apatite-containing biocomposites have been developed using PVA [198,[291][292][293][294], PVAP [295], and other polymers [296][297][298][299][300][301][302][303][304][305][306]. Namely, PVA/CDHA biocomposite blocks were prepared by precipitating CDHA in an aqueous solution of PVA [198].…”