Do bio-insecticides affect only insect species? Behavior, regeneration, and sexual reproduction of a non-target freshwater planarian

Silva, Laila Cristina Rezende; Dornelas, Aline Silvestre Pereira; Saraiva, Althiéris de Souza; Gravato, Carlos; Pestana, João L.T.; Soares, Amadeu Mortágua Velho da Maia; Sarmento, Renato Almeida

doi:10.1007/s11356-021-16493-8

Cited by 7 publications

(5 citation statements)

References 69 publications

(39 reference statements)

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“…3 However, there is less knowledge about the potential ecological effects of pesticides on fresh-water ecosystems, compared to that on the effects of pesticides on terrestrial non-target species. 4 Avermectins (AVMs) are a group of 16-membered macrocyclic lactones, that was registered as insecticides (e.g., abamectin, ABM) and endectocides (e.g., ivermectin, IVM) since 1980s. 5 The most widely recognized mode of action of AVMs in invertebrates is the overstimulation of the glutamate-gated chloride channels (GluCls), and their blocking of the γ-Aminobutyric acid receptors (GABARs) in insects.…”

Section: Introductionmentioning

confidence: 99%

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Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

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BACKGROUND: Pesticides are extensively applied globally. Pesticide residues induce calamitous effects on the environment and untargeted organisms. Public concerns for the safety of freshwater organisms and the challenges posed by aquatic contaminants remain high. In the present study, the acute toxicity of avermectins (AVMs) to the crayfish, Procambarus clarkii was evaluated. We also evaluated the potential effects of AVM on the biochemical and transcriptomic status of the hepatopancreas and gastrointestinal tract in P. clarkii. RESULTS:The 24, 48, 72, 96 h median lethal concentrations (LC 50 ) of AVM on crayfish were 2.626, 1.162, 0.723, 0.566 mg L −1 , respectively. The crayfish were then exposed to 0.65 mg L −1 of AVM for 96 h. AVM significantly altered biochemical parameters including AChE and CAT activities in the hepatopancreas, and AChE, SOD and Na + -K + -ATPase activities in the gastrointestinal tract at several time points. Furthermore, transcriptomic analysis identified 953 and 1851 differentially-expressed genes (DEGs) in the hepatopancreas and gastrointestinal tract, respectively. KEGG enrichment showed that the gene expression profiles of the hepatopancreas and gastrointestinal tract were distinct from each other. The DEGs in the hepatopancreas were mostly enriched with stress-response pathways, while the majority of the DEGs in the gastrointestinal tract belonged to metabolism-related pathways.CONCLUSION: We demonstrated that the AVM induced acute toxicity, oxidative stress, osmoregulation disturbance, neurotoxicity and transcriptome imbalance in crayfish. These findings unraveled the detrimental effects of AVMs exposure on crayfish.

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Section: Introductionmentioning

confidence: 99%

“…This is due to the detection of pesticides in adjacent water bodies via direct water draining from paddy fields or from soil leaching 3 . However, there is less knowledge about the potential ecological effects of pesticides on fresh‐water ecosystems, compared to that on the effects of pesticides on terrestrial non‐target species 4 …”

Section: Introductionmentioning

confidence: 99%

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

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“…Untreated worms demonstrated highest motility, suggesting an intact microbiome may influence motility and possibly withdrawal-like behavior. Silva found that addition of certain bacteria, namely the gram positive rod Bacillus thuringiensis kurstaki , significantly diminished G. tigrina motility (Silva et al 2022). It is therefore possible that removal of certain microbiome populations via administration of broad spectrum antibiotics could similarly alter motility.…”

Section: Discussionmentioning

confidence: 99%

“…Planarian flatworms demonstrate a complex ability to learn and problem-solve and can completely regenerate their brain and central nervous system if damaged (Morokuma, et al 2017). Administration of Bacillus-based bioinsecticides have recently been shown to alter behavior and regeneration rates planaria (Silva, et al 2022). This suggests the microbiome could play an important role in the modulation of behavior in response to chemical, such as pharmacological agents.…”

Section: Introductionmentioning

confidence: 99%

Influence of Microbiome on Withdrawal-Like Behavior in Planaria

Sosa,

Pisciotta,

Guerra-Solano

et al. 2023

Preprint

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The microbiome of animals can influence health status, disease susceptibility and can also influence host behavior. This study investigates the effect of microbiome alteration on animal behavior using the planarian model system. For this,Girardia tigrina(Brown Planaria) andPhagocata gracilis(Black Planaria) were investigated. These flatworms possess a primitive nervous system and exhibit similar addictive and withdrawal behaviors to mammals. Here we tested the hypothesis that alteration of planarian microbiome using the broad-spectrum antibiotics kanamycin and ampicillin could influence the worms’ behavior and withdrawal response to nicotine. After antibiotic treatment period of seven days, the behavior and withdrawal response of four groups of brown and black planaria was analyzed by recording the worms’ motility on a 1 cm2grid. Results support the hypothesis as treatment significantly altered behavior in black worms. The microbiomes of antibiotic treated planaria were analyzed using conventional culture techniques, microscopy and metagenomic methods. Alpha proteobacteria including Sphingomonadaceae were detected in both Brown and Black planarians. This study suggests chemotherapeutic alteration of an animal’s microbiome can influence motility behavior and withdrawal responses to addictive substance and sheds light on the species composition of Planarian microbiomes.

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“…Although the potential for automatisation is clear, most toxicological research on planarians has focused on a small number of substances and employed more traditional methodologies. These approaches have been used to assess the impact of insecticides, pesticides, metals, and neurotoxic compounds (Simao et al ., 2020; Dornelas et al ., 2021; Silva et al ., 2022).…”

Section: Non‐standard Modelsmentioning

confidence: 99%

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

Rosner,

Ballarin,

Barnay‐Verdier

et al. 2023

Biological Reviews

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Aquatic invertebrates play a pivotal role in (eco)toxicological assessments because they offer ethical, cost‐effective and repeatable testing options. Additionally, their significance in the food chain and their ability to represent diverse aquatic ecosystems make them valuable subjects for (eco)toxicological studies. To ensure consistency and comparability across studies, international (eco)toxicology guidelines have been used to establish standardised methods and protocols for data collection, analysis and interpretation. However, the current standardised protocols primarily focus on a limited number of aquatic invertebrate species, mainly from Arthropoda, Mollusca and Annelida. These protocols are suitable for basic toxicity screening, effectively assessing the immediate and severe effects of toxic substances on organisms. For more comprehensive and ecologically relevant assessments, particularly those addressing long‐term effects and ecosystem‐wide impacts, we recommended the use of a broader diversity of species, since the present choice of taxa exacerbates the limited scope of basic ecotoxicological studies.This review provides a comprehensive overview of (eco)toxicological studies, focusing on major aquatic invertebrate taxa and how they are used to assess the impact of chemicals in diverse aquatic environments. The present work supports the use of a broad‐taxa approach in basic environmental assessments, as it better represents the natural populations inhabiting various ecosystems. Advances in omics and other biochemical and computational techniques make the broad‐taxa approach more feasible, enabling mechanistic studies on non‐model organisms. By combining these approaches with in vitro techniques together with the broad‐taxa approach, researchers can gain insights into less‐explored impacts of pollution, such as changes in population diversity, the development of tolerance and transgenerational inheritance of pollution responses, the impact on organism phenotypic plasticity, biological invasion outcomes, social behaviour changes, metabolome changes, regeneration phenomena, disease susceptibility and tissue pathologies. This review also emphasises the need for harmonised data‐reporting standards and minimum annotation checklists to ensure that research results are findable, accessible, interoperable and reusable (FAIR), maximising the use and reusability of data. The ultimate goal is to encourage integrated and holistic problem‐focused collaboration between diverse scientific disciplines, international standardisation organisations and decision‐making bodies, with a focus on transdisciplinary knowledge co‐production for the One‐Health approach.

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Do bio-insecticides affect only insect species? Behavior, regeneration, and sexual reproduction of a non-target freshwater planarian

Cited by 7 publications

References 69 publications

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Influence of Microbiome on Withdrawal-Like Behavior in Planaria

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

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