Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p

Abstract

Summary Yeast peroxisomes multiply by fission. Fission requires two dynamin-related proteins, Dnm1p and Vps1p. We show that Dnm1p-dependent peroxisome fission requires Fis1p, Caf4p and Mdv1p using an in vivo fission assay. Fluorescence microscopy of cells expressing GFP-tagged Caf4p and Mdv1p revealed that their association with peroxisomes relies on Fis1p. Vps1p-dependent peroxisome fission occurs independently of these factors. Vps1p contributes most to fission of peroxisomes when cells are grown on glucose. Overexpression of Dnm1p suppresses the fission defect as long as Fis1p and either Mdv1p or Caf4p are present. Conversely, overexpression of Dnm1p does not restore the vacuolar fusion defect of vps1 cells, and Vps1p overexpression does not restore the mitochondrial fission defect of dnm1 cells. These data show that Vps1p and Dnm1p are part of independent fission machineries. Since the contribution of Dnm1p to peroxisome fission appears to be more pronounced in cells that proliferate peroxisomes in response to mitochondrial dysfunction, Dnm1p might be part of the mechanism that coordinates mitochondrial and peroxisomal biogenesis.

The progression of peroxisomal degradation through autophagy requires peroxisomal division

“…Dnm1 is able to constrict membrane, and its proper function and localization on the peroxisomes requires a fission complex, which also includes Fis1, Mdv1, and Caf4. 13 Fis1 is a tail-anchored membrane receptor that interacts with the partially redundant proteins Mdv1 and Caf4, which in turn bind to Dnm1, to recruit the latter to the peroxisome. This fission complex is shared with mitochondria, and promotes mitochondrial fission through the same mechanism of Dnm1 recruitment.…”

Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae

“…One is that Dnm1 adapter proteins are present. For example, mitochondrial division factors Fis1p and Mdv1 (Mozdy et al, 2000;Tieu and Nunnari, 2000) are required for Dnm1-dependent peroxisomal division (Motley et al, 2008), and human Fis1 serves as the Dnm1 recruiter to peroxisomes (Koch et al, 2005). Fis1p and Mdv1 are conserved in C. merolae (Matsuzaki et al, 2004), and there are >20 candidates that could be components of POD machinery .…”

“…Dnm1 is responsible for the division of the peroxisome and mitochondrion that is mediated by GTPase activity and is highly conserved in plant, yeast and mammalian cells (Bleazard et al, 1999;Smirnova et al, 2001;Sesaki and Jensen, 1999;Koch et al, 2003;Li and Gould, 2003;Mano et al, 2004;Tanaka et al, 2006;Motley and Hettema, 2007). Importantly, Dnm1-associated proteins such as fission1 (Fis1) (Mozdy et al, 2000), mitochondrial division (Mdv1) (Tieu and Nunnari, 2000) and mitochondrial fission factor (Mff ) (GandreBabbe and van der Bliek, 2008) are also used in both divisions of peroxisomes and mitochondria (Motley and Hettema, 2007;Motley et al, 2008). Moreover, Dnm1 is phylogenetically linked to Dnm2/ DRP5B, which is involved in plastid division , and dynamin-1, which is involved in synaptic vesicle endocytosis (Hinsaw and Schmid, 1995).…”

Programmed Cell Death Initiation and Execution in Budding Yeast

“…Mitochondrial fission requires the GTPase Dnm1, which forms atypical helical filaments that first encircle, then constrict, mitochondria until scission is achieved (Otsuga et al 1998;Bleazard et al 1999;Sesaki and Jensen 1999). Recruitment of Dnm1 to the mitochondrial outer membrane (MOM) requires the receptor Fis1 (Mozdy et al 2000;Tieu et al 2002) and one of two adaptors, Mdv1 (Mozdy et al 2000;Tieu et al 2002;Cerveny and Jensen 2003) or Caf4 (Schauss et al 2006;Motley et al 2008). Interestingly, peroxisome fission also requires Fis1 and one of two dynamin-like proteins, Vps1 or Dnm1; the latter seems only important in cultures grown on oleic acid (Hoepfner et al 2001;Kuravi et al 2006).…”