DNAzyme biosensors for the detection of pathogenic bacteria

Ma, Xiaoyi; Ding, Wen; Wang, Cang; Wu, Hangjie; Tian, Xiaopeng; Lyu, Mingsheng; Wang, Shujun

doi:10.1016/j.snb.2020.129422

Cited by 61 publications

(31 citation statements)

References 86 publications

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“…So far, several methods such as plate culture method, enzyme-linked immunosorbent assay, molecular biological detection, for example, polymerase chain reaction, have been focused on the determination of foodborne pathogens in different matrices. These methods not only require expensive instruments and highly skilled workers but also involve intricate sample pretreatments and are time-consuming, − hence limiting their application in food safety and security. Consequently, it is particularly important to establish a simple, sensitive, and specific method for the quantitative detection of S.…”

Section: Introductionmentioning

confidence: 99%

SERS Sensors Based on Aptamer-Gated Mesoporous Silica Nanoparticles for Quantitative Detection of Staphylococcus aureus with Signal Molecular Release

et al. 2021

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This work describes a simple and novel biosensor for the quantitative determination of Staphylococcus aureus (S. aureus) based on target-induced release of signal molecules from aptamer-gated aminated mesoporous silica nanoparticles (MSNs) coupled with surface-enhanced Raman scattering (SERS) technology. MSNs were synthesized and then modified with amino groups by (3-aminopropyl) triethoxysilane to make them positively charged. Next, signal molecules (4-aminothiophenol, 4-ATP) were loaded into the pores of MSNs. Then, negatively charged aptamers of S. aureus were assembled on the surface of MSNs through electrostatic interactions. Upon the addition of S. aureus, the assembled aptamers were specifically bound to the bacteria. Consequently, the “gates” were opened, resulting in the release of 4-ATP from the pores of MSNs. The released molecules were measured by a Raman spectrometer, and the intensity of 4-ATP at 1071 cm–1 was linearly related to the S. aureus concentration. A silver nanoflower silica core–shell structure (Ag NFs@SiO2) was prepared and it served as a SERS substrate. Under optimized experimental conditions, a good linear relationship (y = 2107.93 + 1536.30x, R 2 = 0.9956) in the range from 4.7 × 10 to 4.7 × 108 cfu/mL was observed with a limit of detection of 17 cfu/mL. The method was successfully applied for the analysis of S. aureus in fish samples and the recovery rate was 91.3–109%.

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Section: Introductionmentioning

confidence: 99%

SERS Sensors Based on Aptamer-Gated Mesoporous Silica Nanoparticles for Quantitative Detection of Staphylococcus aureus with Signal Molecular Release

et al. 2021

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“…Recently, more simple, sensitive, and cost-effective methods were progressed for the detection of pathogens [ 32 , 33 , 34 ]. In addition, highly sensitive and inexpensive techniques have been developed for bacterial capture [ 1 , 35 ]. Moreover, the outcomes are constantly unsatisfactory, primarily because pathogens commonly exist in complex samples in the environment [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

“…Pathogenic bacteria cause environmental contamination and serious diseases and have become a significant public health burden over time [ 1 ]. These pathogens responsible for many diseases, such as septicemia, meningitis, gastroenteritis, and abortion, which are all associated with a high mortality rate [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Vancomycin-Loaded Furriness Amino Magnetic Nanospheres for Rapid Detection of Gram-Positive Water Bacterial Contamination

et al. 2022

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Bacterial pathogens pose high threat to public health worldwide. Different types of nanomaterials have been synthesized for the rapid detection and elimination of pathogens from environmental samples. However, the selectivity of these materials remains challenging, because target bacterial pathogens commonly exist in complex samples at ultralow concentrations. In this study, we fabricated novel furry amino magnetic poly-L-ornithine (PLO)/amine-poly(ethylene glycol) (PEG)-COOH/vancomycin (VCM) (AM-PPV) nanospheres with high-loading VCM for vehicle tracking and the highly efficient capture of pathogens. The magnetic core was coated with organosilica and functionalized with cilia. The core consisted of PEG/PLO loaded with VCM conjugated to Gram-positive bacterial cell membranes, forming hydrogen bonds with terminal peptides. The characterization of AM-PPV nanospheres revealed an average particle size of 56 nm. The field-emission scanning electron microscopy (FE-SEM) micrographs showed well-controlled spherical AM-PPV nanospheres with an average size of 56 nm. The nanospheres were relatively rough and contained an additional 12.4 nm hydrodynamic layer of PLO/PEG/VCM, which provided additional stability in the suspension. The furry AM-PPV nanospheres exhibited a significant capture efficiency (>90%) and a high selectivity for detecting Bacillus cereus (employed as a model for Gram-positive bacteria) within 15 min, even in the presence of other biocompatible pathogens. Moreover, AM-PPV nanospheres rapidly and accurately detected B. cereus at levels less than 10 CFU/mL. The furry nano-design can potentially satisfy the increasing demand for the rapid and sensitive detection of pathogens in clinical and environmental samples.

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“…The traditional culture-based methods are time consuming and labor intensive (Ma et al, 2021), and such methods cannot detect bacteria in the viable but nonculturable (VBNC) state because VBNC cells cannot grow on plates, but they retain virulence or can recover virulence after resuscitation (Li et al, 2014). In addressing the bottlenecks of the culture-based method, PCR has been developed in the foodborne pathogen detection field (Gaňová et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Real-time recombinase-aided amplification with improved propidium monoazide for the rapid detection of viable Escherichia coli O157:H7 in milk

Zhou²,

Xie

et al. 2022

Journal of Dairy Science

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Escherichia coli O157:H7, the causative agent of thrombotic thrombocytopenic purpura and hemolytic uremic syndrome in humans, generates a effective harm to community health because of its high pathogenicity. A real-time recombinase-aided amplification (rRAA) is an emerging method for nucleic acid detection. However, genomic DNA of bacteria could exist in food and the environment for a long time after death and could be amplified by rRAA assay, resulting in false-positive signal; thus, developing a fast and sensitive method is necessary to detect viable foodborne pathogens in food products. In our research, rRAA assay coupled with an enhanced nucleic acid binding dye named improved propidium monoazide (PMAxx) was established and applied in viable E. coli O157:H7 identification in skim milk. The PMAxx could eliminate interference from dead bacteria by permeating impaired membranes and covalently linking to DNA to prevent DNA amplification. The PMAxx-rRAA assay was performed with high sensitivity and good specificity. The PMAxx-rRAA assay could detect as low as 5.4 × 10 0 cfu/mL of viable E. coli O157:H7 in pure culture, and 7.9 × 10 0 cfu/mL of viable E. coli O157:H7 in skim milk. In addition, the PMAxx-rRAA assay was performed in the presence of a high concentration of dead bacteria or nontarget bacteria in skim milk to verify the capacity to resist interference from dead bacteria and nontarget bacteria. Therefore, the established PMAxx-rRAA assay is a valuable tool for the identification of viable E. coli O157:H7 in complex food matrix.

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DNAzyme biosensors for the detection of pathogenic bacteria

Cited by 61 publications

References 86 publications

SERS Sensors Based on Aptamer-Gated Mesoporous Silica Nanoparticles for Quantitative Detection of Staphylococcus aureus with Signal Molecular Release

SERS Sensors Based on Aptamer-Gated Mesoporous Silica Nanoparticles for Quantitative Detection of Staphylococcus aureus with Signal Molecular Release

Vancomycin-Loaded Furriness Amino Magnetic Nanospheres for Rapid Detection of Gram-Positive Water Bacterial Contamination

Real-time recombinase-aided amplification with improved propidium monoazide for the rapid detection of viable Escherichia coli O157:H7 in milk

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