DNAzyme-Based Lithium-Selective Imaging Reveals Higher Lithium Accumulation in Bipolar Disorder Patient-Derived Neurons

McGhee, Claire E.; Yang, Zhenglin; Guo, Weijie; Wu, Yuting; Lyu, Mingkuan; DeLong, Cynthia J.; Hong, Shanni; Ma, Yuan; McInnis, Melvin G.; O’Shea, K. Sue; Lu, Yi

doi:10.1021/acscentsci.1c00843

Cited by 39 publications

(35 citation statements)

References 69 publications

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“…While glucose is non‐charged, salt can bridge the highly negatively charged aptamer to promote aptamer binding. A DNA aptamer motif for specific binding of Na + is already known, [36] and DNAzymes specific for Na + and Li + were also reported [37,38] . Thus, it is understandable that this aptamer can achieve specific Na + ‐mediated glucose binding.…”

Section: Resultsmentioning

confidence: 92%

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2‐Aminopurine Fluorescence Spectroscopy for Probing a Glucose Binding Aptamer

Huang

Zheng

et al. 2022

ChemBioChem

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Glucose is the most important analyte for biosensors. Recently a DNA aptamer was reported allowing binding-based detection. However, due to a relatively weak binding affinity, it is difficult to perform binding assays to understand the property of this aptamer. In this work, we replaced the only adenine base in the aptamer binding pocket with a 2-aminopurine (2AP) and used fluorescence spectroscopy to study glucose binding. In the selection buffer, glucose increased the 2AP fluorescence with a K d of 15.0 mM glucose, which was comparable with the 10 mM K d previously reported using the strand displacement assay. The binding required two Na + ions or one Mg 2 + that cannot be replaced by Li + or K + . The binding was weaker at higher temperature and its van't Hoff plot indicated enthalpy-driven binding. While other monosaccharides failed to achieve saturated binding even at high concentrations, two glucosecontaining disaccharides, namely trehalose and sucrose, reached a similar fluorescence level as glucose although with over 10-fold higher K d values. Detection limits in both the selection buffer (0.9 mM) and in artificial interstitial fluids (6.0 mM) were measured.

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Section: Resultsmentioning

confidence: 92%

“…A DNA aptamer motif for specific binding of Na + is already known, [36] and DNAzymes specific for Na + and Li + were also reported. [37,38] Thus, it is understandable that this aptamer can achieve specific Na + -mediated glucose binding.…”

Section: Two Sodium Ions Are Required For Glucose Bindingmentioning

confidence: 99%

2‐Aminopurine Fluorescence Spectroscopy for Probing a Glucose Binding Aptamer

Huang

Zheng

et al. 2022

ChemBioChem

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“…They then demonstrated the fluorescence detection of TPP in whole blood via the mix-and-read operation. In addition, McGhee et al reported the selection and development of a Li + -specific DNAzyme sensor with >100-fold selectivity compared with other biorelevant metal ions [ 56 ]. As shown in Figure 4 b, the fluorescent sensor is designed for imaging of Li + in HeLa cells, human neuronal progenitor cells, and neurons.…”

Section: Native Nucleic Acidsmentioning

confidence: 99%

Nucleic Acids and Their Analogues for Biomedical Applications

Wang

Chu

et al. 2022

Biosensors

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Nucleic acids are emerging as powerful and functional biomaterials due to their molecular recognition ability, programmability, and ease of synthesis and chemical modification. Various types of nucleic acids have been used as gene regulation tools or therapeutic agents for the treatment of human diseases with genetic disorders. Nucleic acids can also be used to develop sensing platforms for detecting ions, small molecules, proteins, and cells. Their performance can be improved through integration with other organic or inorganic nanomaterials. To further enhance their biological properties, various chemically modified nucleic acid analogues can be generated by modifying their phosphodiester backbone, sugar moiety, nucleobase, or combined sites. Alternatively, using nucleic acids as building blocks for self-assembly of highly ordered nanostructures would enhance their biological stability and cellular uptake efficiency. In this review, we will focus on the development and biomedical applications of structural and functional natural nucleic acids, as well as the chemically modified nucleic acid analogues over the past ten years. The recent progress in the development of functional nanomaterials based on self-assembled DNA-based platforms for gene regulation, biosensing, drug delivery, and therapy will also be presented. We will then summarize with a discussion on the advanced development of nucleic acid research, highlight some of the challenges faced and propose suggestions for further improvement.

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“…Lu group reported the first example of NaA43 DNAzyme that was highly specific for the monovalent metal ions, Na + [17] . Very recently, by using the same approach of in vitro selection, they discovered another DNAzyme named 20–4 that was selective to Li + [18] . The Liu group also made efforts to select DNAzymes that were specific to monovalent and trivalent metal ions.…”

Section: Expanding Of Dnazyme Librarymentioning

confidence: 99%

“…Despite the success in divalent metal ion analysis, the sensitive and selective for monovalent ion detection is still a challenge for DNAzyme‐based sensors. Recently, the Lu group reported an RNA‐cleaving DNAzyme with high selectivity toward Li + [18] . This Li + ‐specific DNAzyme could achieve over 100‐fold selectivity compared to other biorelevant metal ions, including the two main monovalent metal ions K + and Na + in biological samples.…”

Section: Dnazyme‐based Sensing Applicationsmentioning

confidence: 99%

Recent Advances on DNAzyme‐Based Sensing

Huang

Wang

et al. 2022

Chemistry An Asian Journal

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Owing to the high sensitivity, excellent programmability, and flexible obtainment through in vitro selection, RNA‐cleaving DNAzymes have attracted increasing interest in developing DNAzyme‐based sensors. In this review, we summarize the recent advances on DNAzyme‐based sensing applications. We initially conclude two general strategies to expand the library of DNAzmes, in vitro selection to discover new DNAzymes towards different targets of interest and chemical modifications to endue the existing DNAzymes with new function or properties. We then discuss the recent applications of DNAzyme‐based sensors for the detection of a variety of important biomoleucles both in vitro and in vivo. Finally, perspectives on the challenges and future directions in the development of DNAzyme‐based sensors are provided.

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DNAzyme-Based Lithium-Selective Imaging Reveals Higher Lithium Accumulation in Bipolar Disorder Patient-Derived Neurons

Cited by 39 publications

References 69 publications

2‐Aminopurine Fluorescence Spectroscopy for Probing a Glucose Binding Aptamer

2‐Aminopurine Fluorescence Spectroscopy for Probing a Glucose Binding Aptamer

Nucleic Acids and Their Analogues for Biomedical Applications

Recent Advances on DNAzyme‐Based Sensing

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