DNASER. I. Layout and data analysis

Abstract

We present the DNA analyzer (DNASER), a novel bioinstrumentation for real-time acquisition and elaboration of images from fluorescent DNA microarrays. A white light beam illuminates the target sample allowing the images grabbing on a high sensibility and wide-band charge-coupled device camera (ORCA II-Hamamatsu). This high-performance device permits to acquire images faster and of higher quality than the traditional systems. The DNA microarrays images are processed to recognize the DNA chip spots, to analyze their superficial distribution on the glass slide and to evaluate their geometric and intensity properties. Differently form conventional techniques, the spots analysis is fully automated and the DNASER does not require any additional information about the DNA microarray geometry. The DNASER hardware and software architecture is illustrated. Preliminary results are shown from experiments performed on real DNA samples.

Gene expression in the cell cycle of human T lymphocytes: I. Predicted gene and protein networks

“…This can be very useful in the therapy of several kinds of tumors. Moreover, the objective identification of leader genes and of gene interaction maps can suggest a more rational approach to experimental techniques and methods, as DNA microarrays have emerged to be a very powerful tool for gene expression analysis not only for the study of cell cycle [Butte, 2002;Nicolini et al, 2002;Nicolini et al, 2005]. Anyway, microarrays often display a very large number of genes, usually several thousand.…”

Psychodrama: helping families to adapt to childhood diabetes

“…We employed a novel bioinstrumentation for real‐time acquisition and elaboration of images from fluorescent DNA and protein microarrays realized in our laboratories, the DNASER (Figure 1), acronym of DNA analySER (equipped with the ORCA II Hamamatsu CCD camera),5 to analyze gene microarrays. In a separate communication (Sivozhelezov et al), we present the results obtained analyzing a self‐assembling protein microarray of new conception, called nucleic acid programmable protein array (NAPPA), by DNASER.…”

“…This review summarizes here its major features with few key examples of applications to medicine and cell biology, which make clear its differentiation and advancement with respect to classical genomic defined in all textbooks as the identification, isolation, and characterization of cell genes within the framework of traditional current biomedicine. DNA microarrays have emerged as one of the most promising methods for the analysis of gene expression 4,5. This technique allows the study of an immense amount of genes (actually the entire 35,000 genes or more of the human genome) with only one experiment and therefore can draw a picture of a whole genome.…”

Two-dimensional singlet oxygen imaging with its near-infrared luminescence during photosensitization

“…The linear fitting analysis gave an r 2 of 0.993. We could calculate the detection sensitivity of the system as follos: 23,24 …”