DNA Vaccines: A Review

Pd, Lewis; La, Babiuk

doi:10.1016/s0065-3527(08)60367-x

Cited by 83 publications

(31 citation statements)

References 187 publications

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Section: Introductionmentioning

confidence: 99%

“…The RNA selfamplifying property is of considerable interest with respect to vaccine biosafety: the vector replicates at the RNA level and not at the DNA level, so the rate of foreign DNA present in vivo and possessing 'genome integration potential' is controlled and does not increase following vaccination (contrary to some attenuated or recombinant vaccines). Furthermore, when a suicidal DNA vaccine is transfected into cells, it leads eventually to apoptosis of the transfected cells (Kohno et al, 1998;Leitner et al, 2000), which is particularly important in alleviating the concerns of potential integration and cell transformation generated by the use of conventional DNA vaccines (Gurunathan et al, 2000;Lewis & Babiuk, 1999).Several groups have demonstrated the ability of suicidal DNA vaccines to induce high-level humoral and cellmediated immunity against a variety of antigens, with immunized animals developing more prominent immune responses than those receiving a conventional DNA vaccine encoding the same antigen (Berglund et al, 1998;Deshpande et al, 2002;Hariharan et al, 1998;Kirman et al, 2003;Leitner et al, 2000). In addition, it has been demonstrated that suicidal DNA vaccines could break immunological tolerance by activating innate antiviral pathways, in contrast to conventional DNA vaccines encoding the same antigen (Leitner et al, 2003).…”

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confidence: 99%

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Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

Sun

Liu

Guo

et al. 2007

Journal of General Virology

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A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against swine vesicular disease virus (SVDV). The 1BCD gene of SVDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/1BCD, was transfected into BHK-21 cells and the antigenicity of the expressed protein was confirmed using an indirect immunofluorescence assay. Immunogenicity was studied in guinea pigs and swine. Animals were injected intramuscularly three times with pSCA/1BCD at regular intervals. Anti-SVDV antibodies were detected by ELISA, the lymphocyte proliferation response was tested by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide method and neutralizing antibodies were measured by microneutralization tests. The data showed that SVDV-specific antibodies, neutralizing antibodies and lymphocyte proliferation were induced in both guinea pigs and swine. Furthermore, after three successive vaccinations with pSCA/1BCD, half of the pigs were protected against challenge with SVDV. These results should encourage further work towards the development of a DNA vaccine against SVDV. INTRODUCTIONSwine vesicular disease (SVD) is a highly contagious viral pig disease, characterized by the appearance of vesicles on the coronary bands, heels of the feet and less commonly on the snout and tongue. Due to the similarity of these lesions to those caused by foot-and mouth-disease (FMD), SVD is subject to international controls and is listed by the World Organization for Animal Health. SVD was first identified in Italy in 1966(Nardelli et al., 1968 and several more outbreaks have been reported subsequently in Europe and eastern Asia (Brocchi et al., 1997). However, in the recent past, reports of SVD have been limited to Portugal and Italy.SVD vaccines have been developed previously to control the disease, both in monovalent form (Gourreau et al., 1975) and in combination with FMD (Mitev et al., 1978), and an SVD subunit vaccine has also been described, although it was not very efficacious (Jiménez-Clavero et al., 1998). Although the inactivated virus vaccines are effective in protecting against clinical signs, there has been little, if any, assessment made of their ability to reduce wild-type virus transmission and no effective vaccine is available commercially. Once introduced, SVD could be a difficult disease to eradicate and improved methods of control would be highly beneficial, including the development of safer and more effective vaccines to protect and control this disease.Suicidal DNA vaccines, based on the alphavirus replicon, have emerged as an important strategy to enhance immunogenicity and to improve the biosafety of conventional DNA vaccines (Berglund et al., 1998;Leitner et al., 1999;Lundstrom, 2000). Unlike the conventional DNA vaccine construct in which heterologous gene expression is driven directly by the RNA polymerase II-dependent promoter, suicidal DNA vaccines based on the replicon of alphaviruses, including Sindbis viru...

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

Sun

Liu

Guo

et al. 2007

Journal of General Virology

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“…During the last decade a fast development of methodology concerning nucleic acid based immunization has occurred, including experimental vaccine applications in a large number of viral, bacterial and parasitic systems [1,2]. Safety aspects with naked DNA vaccines, mainly concerning possible chromosomal integration and induction of immunological tolerance [2], have incited the development of nonpersistent vectors, such as RNA-based vaccines [3] and suicidal DNA vaccines [4].…”

Section: Introductionmentioning

confidence: 99%

“…Safety aspects with naked DNA vaccines, mainly concerning possible chromosomal integration and induction of immunological tolerance [2], have incited the development of nonpersistent vectors, such as RNA-based vaccines [3] and suicidal DNA vaccines [4]. In this context, the SFV replicon has made it possible to construct vectors which may direct the high-level, transient expression of heterologous proteins in vivo [4,5].…”

Section: Introductionmentioning

confidence: 99%

Comparative Immunization Study Using RNA and DNA Constructs Encoding a Part of the Plasmodium falciparum Antigen Pf332

et al. 2001

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Development of nucleic acid-based vaccines against parasitic diseases shows great promise, although certain concerns about safety aspects of conventional DNA vaccines have been raised. This study presents a comparison of antibody responses induced in mice by DNA and RNA-based immunization with vectors encoding a part of the P. falciparum antigen Pf332. Two types of plasmids were used, one conventional DNA plasmid containing a cytomegalovirus promoter and one suicidal DNA plasmid encoding the Semliki Forest virus (SFV) replicase. RNA, encoding the SFV replicase and the relevant antigen, was delivered either as naked RNA or packaged in SFV suicide particles. In general, the antibody responses induced by the DNA plasmids were low and peaking after three injections, the conventional plasmid giving the highest responses. Also the RNA delivered in SFV particles consistently induced antibody responses, although comparatively low. Analyses of the ratio of immunoglobulin (Ig)G1/IgG2a subclasses in the responses indicated that all plasmids resulted in a bias for a Th2-type of response, while the SFV-particles elicited a Th1 type of response. Importantly, all these immunogens induced an immunological memory, which could be efficiently activated by a booster injection with the corresponding protein, with unchanged patterns of IgG subclasses.

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“…Several investigators have demonstrated the feasibility of using a direct injection of plasmid DNA for the induction of protective immunity against various pathogens and the production of specific antibodies [3][4][5] . Recently, phage display technology has been developed and has proven to be a very powerful technique for production of proteins or peptides.…”

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confidence: 99%

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Boonmuen¹,

Tayapiwatana²,

Kasinrerk³

2005

ScienceAsia

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ABSTRACT:Immunization with two different immunogens, i.e., plasmid encoding CD147 (pCDM8-CD147) and phage-displayed CD147 were compared for producing polyclonal antibodies. Two mice were injected with pCDM8-CD147 or phage-displayed CD147 at bi-weekly intervals and antibody responses were determined by indirect ELISA and immunofluorescence. The anti-CD147 antibodies could be detected in the immunized sera after inoculating with either pCDM8-CD147 or phage-displayed CD147. However, the antibody response induced by phage-displayed CD147 was much higher than that by pCDM8-CD147. These results suggested the possibility of using the phage display technique for preparing hyperimmune serum when purified protein antigens are difficult to obtain.

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DNA Vaccines: A Review

Cited by 83 publications

References 187 publications

Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

Comparative Immunization Study Using RNA and DNA Constructs Encoding a Part of the Plasmodium falciparum Antigen Pf332

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