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Abstract

ABSTRACT:Immunization with two different immunogens, i.e., plasmid encoding CD147 (pCDM8-CD147) and phage-displayed CD147 were compared for producing polyclonal antibodies. Two mice were injected with pCDM8-CD147 or phage-displayed CD147 at bi-weekly intervals and antibody responses were determined by indirect ELISA and immunofluorescence. The anti-CD147 antibodies could be detected in the immunized sera after inoculating with either pCDM8-CD147 or phage-displayed CD147. However, the antibody response induced by phage-displayed CD147 was much higher than that by pCDM8-CD147. These results suggested the possibility of using the phage display technique for preparing hyperimmune serum when purified protein antigens are difficult to obtain.