DNA triplexes and regulation of the c-myc gene

Kinniburgh, Alan J.; Firulli, Anthony B.; Kolluri, Rukmini

doi:10.1016/0378-1119(94)90416-2

Cited by 37 publications

(17 citation statements)

References 13 publications

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“…In the presence of rYB-1, MBN produced a distinct banding pattern that was confined to the 3Ј IR (lane 3) with no banding in the Y-box motif. Nuclear extracts from quiescent and serum-stimulated mesangial cells induced a similar banding pattern to that observed with rYB-1, which was enhanced with prolonged serum exposure (lane [4][5][6]. Strong bands at the 3Ј-end indicated the presence of exonuclease activity.…”

Section: Nuclear Protein Binding Regions Within the ϫ220 To ϩ115supporting

confidence: 58%

“…Regulation of collagen gene expression occurs primarily at the level of transcription (2)(3)(4). A number of regulatory elements required for constitutive and inducible expression have been identified in the promoter and first intron of both the COL1A1 and COL1A2 genes (2,5,6) with gene transcription controlled by the complex interplay of positive and negative regulatory factors.…”

mentioning

confidence: 99%

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The Y-box Binding Protein YB-1 Suppresses Collagen α1(I) Gene Transcription via an Evolutionarily Conserved Regulatory Element in the Proximal Promoter

Norman¹,

Lindahl²,

Shakib³

et al. 2001

Journal of Biological Chemistry

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Section: Nuclear Protein Binding Regions Within the ϫ220 To ϩ115supporting

confidence: 58%

mentioning

confidence: 99%

The Y-box Binding Protein YB-1 Suppresses Collagen α1(I) Gene Transcription via an Evolutionarily Conserved Regulatory Element in the Proximal Promoter

Norman¹,

Lindahl²,

Shakib³

et al. 2001

Journal of Biological Chemistry

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“…Many models have been suggested to explain the conformational changes observed in the NHE. The marked disparity in the nucleotide composition of the two strands of the NHE prompted suggestion of an H-DNA structure as a model for the noncanonical NHE structure (13). This H-DNA structure is an intramolecular pyrimidine-purine-pyrimidine triplex.…”

mentioning

confidence: 99%

Induction of Duplex to G-quadruplex Transition in the c-myc Promoter Region by a Small Molecule

Rangan

Fedoroff

Hurley

2001

Journal of Biological Chemistry

194

145

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A major control element of the human c-myc oncogene is the nuclease-hypersensitive purine/pyrimidine-rich sequence. This double-stranded DNA fragment, corresponding to the 27-base pair segment in the nucleasehypersensitive element of the c-myc promoter region, forms a stable Watson-Crick double helix under physiological conditions. However, this duplex DNA can be effectively converted to G-quadruplex DNA by a small molecular weight ligand. Both intermolecular and intramolecular G-quadruplex forms can be induced by this ligand. Similar transitional changes are also observed with the duplex telomeric sequence from the Oxytricha species. These results provide additional support to the idea that G-quadruplex structures may play structural roles in vivo and also provide insight into novel methodologies for rational drug design. These structurally altered DNA elements might serve as regulatory signals in gene expression or in telomere dynamics and hence are promising targets for drug action.The protein product of the c-myc protooncogene plays a vital role in the process of cellular growth and differentiation (1). Deregulation of c-myc expression has been detected in many cancers and is believed to be an important step in tumorigenesis (2). The control of c-myc gene expression is a complex process and occurs at various steps of transcription, such as initiation, elongation, and attenuation, as well as during the post-transcriptional stages. Although the mechanisms involved in this regulation are not yet completely understood, a major control element of the human c-myc oncogene has been localized. This is a purine/pyrimidine-rich region located 115 bases upstream from the P1 promoter, which controls up to 95% of the total c-myc transcription (3, 4). This DNA segment is highly sensitive to DNase I and S1 nuclease (5, 6) and is termed the nuclease-hypersensitive element (NHE).1 The appearance of this hypersensitive site is coupled with transcription activation of the c-myc oncogene. Structural variations in the NHE can influence the binding of transcription factors. Transcription factors such as heterogenous nuclear ribonucleoprotein K and nucleoside diphosphate kinase B (7) bind sequence specifically to the pyrimidine-rich strand of the NHE and activate c-myc transcription (8). The transacting factors heterogenous nuclear ribonucleoprotein A/B (9) and cellular nucleic acid-binding protein (10) bind to the NHE and are shown to augment c-myc expression in vitro. Apart from protein factors, antisense oligonucleotides bind to the NHE and repress c-myc transcription in vitro (11,12). Formation of a colinear triplex between the synthetic oligonucleotide and the NHE was proposed to cause this observed repression in transcription.The NHE has a high potential to form atypical DNA structures under superhelical stress. It was proposed to be in a slow equilibrium between a Watson-Crick base-paired double helix and an atypical DNA structure (6). Many models have been suggested to explain the conformational changes observed in the NHE...

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“…A typical titration of either complex 1 or 2 in phosphate buffer was performed by using a fixed A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 6 either complex 1 or 2 concentration (20 µM), to which the RNA triplex stock solution (2 µL) as gradually added up to saturation. After each addition, the solution should be mixed evenly and allowed to re-equilibrate for at least 5 min before recording the absorption spectra.…”

Section: Electronic Absorption Spectral Studies Of the Bindingmentioning

confidence: 99%

Binding properties of ruthenium(II) complexes [Ru(bpy)2(ppn)]2+ and [Ru(phen)2(ppn)]2+ with triplex RNA: As molecular “light switches” and stabilizers for poly(U)·poly(A)*poly(U) triplex

Sun

Zhu

et al. 2016

Journal of Inorganic Biochemistry

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DNA triplexes and regulation of the c-myc gene

Cited by 37 publications

References 13 publications

The Y-box Binding Protein YB-1 Suppresses Collagen α1(I) Gene Transcription via an Evolutionarily Conserved Regulatory Element in the Proximal Promoter

The Y-box Binding Protein YB-1 Suppresses Collagen α1(I) Gene Transcription via an Evolutionarily Conserved Regulatory Element in the Proximal Promoter

Induction of Duplex to G-quadruplex Transition in the c-myc Promoter Region by a Small Molecule

Binding properties of ruthenium(II) complexes [Ru(bpy)2(ppn)]2+ and [Ru(phen)2(ppn)]2+ with triplex RNA: As molecular “light switches” and stabilizers for poly(U)·poly(A)*poly(U) triplex

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