DNA strand scission induced by a non-thermal atmospheric pressure plasma jet

Ptasińska, Sylwia; Bahnev, Blagovest; Stypczyńska, Agnieszka; Bowden, Mark; Mason, Nigel J.; Braithwaite, Nicholas

doi:10.1039/c001188f

Cited by 67 publications

(60 citation statements)

References 15 publications

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“…Several previous studies have investigated the effect of atmospheric pressure plasma directly on plasmid DNA and shown that atmospheric plasma treatment induced double strand breaks in plasmid DNA Li et al, 2008;O Connell et al, 2011;Ptasin ska et al, 2010 . Investigation into the effect of atmospheric dielectric barrier discharge plasma on plasmid and chromosomal DNA in E. coli cells by agarose gel electrophoresis…”

Section: Discussionmentioning

confidence: 99%

Low-Pressure Plasma Application for the Inactivation of the Seed-borne Pathogen Xanthomonas campestris

et al. 2016

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The aim of this study was to investigate the effect of low-pressure plasma treatment on seed disinfection and the possible mechanisms underlying this effect. Seed-borne disease refers to plant diseases that are transmitted by seeds; seed disinfection is an important technique for prevention of such diseases. In this study, the effectiveness of low-pressure plasma treatment in the inactivation of the seed-borne plant pathogenic bacterium, Xanthomonas campestris, inoculated on cruciferous seeds, was evaluated. The highest inactivation effect was observed when the treatment voltage and argon gas flow rate were 5.5 kV and 0.5 L/min, respectively. The viable cell number of X. campestris was 6.6 log cfu/seed before plasma treatment, and decreased by 3.9 log after 5 min of treatment and by 6.6 log after 40 min. Ethidium monoazide treatment and quantitative real-time PCR results indicated that both the cell membrane and target DNA region were damaged following 5 min of plasma treatment. Although both heat and ozone were generated during the plasma treatment, the contribution of both factors to the inactivation of X. campestris was small by itself in our low-pressure plasma system. Overall, we have shown that our low-pressure plasma system has great applicability to controlling plant pathogenic bacterium contamination of seeds.

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Section: Discussionmentioning

confidence: 99%

Low-Pressure Plasma Application for the Inactivation of the Seed-borne Pathogen Xanthomonas campestris

et al. 2016

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“…Studies using isolated protein and plasmid DNA models have shown protein and DNA damages [95]- [99], and more relevant experiments using bacterial cells have provided direct evidence of breaching and rapture of cell membrane [100], reduction and degradation of proteins [101]- [103], lipid damage [104], and (mostly) single-strand breaks (SSB) of DNA [105]. These results have prompted the suggestion of the involvement of ROS, charged particles, and UV photons [92][100] [105] - [108], with ROS often linked to oxidation of protein and lipid and with UV photons linked to DNA damages.…”

Section: Reactive Plasma Species and Their Biological Targetsmentioning

confidence: 99%

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Kong¹,

Keidar²,

Ostrikov³

2011

J. Phys. D: Appl. Phys.

107

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To cite this version:M G Kong, M Keidar, K Ostrikov. Plasmas meet nanoparticleswhere synergies can advance the frontier of medicine. Journal of Physics D: Applied Physics, IOP Publishing, 2011, 44 (17) AbstractNanoparticles and low-temperature plasmas have been developed, independently and often along different routes, to tackle the same set of challenges in biomedicine. There are intriguing similarities and contrasts in their interactions with cells and living tissues, and these are reflected directly in the characteristics and scope of their intended therapeutic solutions, in particular their chemical reactivity, selectivity against pathogens and cancer cells, safety to healthy cells and tissues, and targeted delivery to diseased tissues. Time has come to ask the inevitable question of possible plasma-nanoparticle synergy and the related benefits to the development of effective, selective, and safe therapies for modern medicine. This perspective article offers a detailed review of the strengths and weakenesses of nanomedicine and plasma medicine as a stand-alone technology, and then provides a critical analysis of some of the major opportunities enabled by synergising the nanotechnology and the plasma technology. It is shown that the plasma-nanoparticle synergy is best captured through the plasma nanotechnology and its benefits for medicine are highly promising.

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“…Generally, decontamination through plasma treatment is accomplished via oxidative stress arisen from synergetic actions of plasma discharge products. [88,103] The specific plasma agent responsible for the mechanism of CAP killing is not clearly corroborated in literature [7,89] ; although CAP is proven to be damaging to bacterial cell walls, primarily due to ROS and RNS. [104] These charged particles have sufficient ability to induce high oxidative stress which cause damage in microbial cells via fast direct interactions.…”

Section: Mechanisms Of Microbial Inactivation By Capmentioning

confidence: 99%

Terminal sterilization: Conventional methods versus emerging cold atmospheric pressure plasma technology for non‐viable biological tissues

Marsit

Sidney

Branch

et al. 2016

Plasma Processes & Polymers

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Tissue products are susceptible to microbial contamination from different sources, which may cause disease transmission upon transplantation. Terminal sterilization using gamma radiation, electron‐beam, and ethylene oxide protocols are well‐established and accepted, however, such methods have known disadvantages associated with compromised tissue integrity, functionality, safety, complex logistics, availability, and cost. Non‐thermal (cold) atmospheric pressure plasma (CAP) is an emerging technology that has several biomedical applications including sterilization of tissues, and the potential to surpass current terminal sterilization techniques. This review discusses the limitations of conventional terminal sterilization technologies for biological materials, and highlights the benefits of utilizing CAP.

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DNA strand scission induced by a non-thermal atmospheric pressure plasma jet

Cited by 67 publications

References 15 publications

Low-Pressure Plasma Application for the Inactivation of the Seed-borne Pathogen <i><i>Xanthomonas campestris</i></i>

Low-Pressure Plasma Application for the Inactivation of the Seed-borne Pathogen <i><i>Xanthomonas campestris</i></i>

Plasmas meet nanoparticles—where synergies can advance the frontier of medicine

Terminal sterilization: Conventional methods versus emerging cold atmospheric pressure plasma technology for non‐viable biological tissues

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