DNA-stabilized silver nanoclusters and carbon nanoparticles oxide: A sensitive platform for label-free fluorescence turn-on detection of HIV-DNA sequences

Ye, Yu-Dan; Li, Xia; Xu, Dangdang; Xing, Xiaojing; Pang, Dai‐Wen; Tang, Hong‐Wu

doi:10.1016/j.bios.2016.06.001

Cited by 86 publications

(26 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carbon dots (CDs) have attracted great interest in chemo-and bio-sensing, theranostic, photocatalysis, bioimaging, light emitting diodes and solar cells [14][15][16][17] due to their attractive fluorescence properties, green and economical synthetic strategy, low cytotoxicity, and feasibility for facile large scale production. Amongst them, the applications of CDs used in biosensing, chemical analysis and environment monitoring especially for potential sensor development were caught much attention due to their favorable photostability, high solubility in aqueous medium, biocompatibility and cost effective [18][19][20][21][22]. Currently, many CDs successfully applied to detect heavy ions in aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution

Ren

Zhang

et al. 2017

Sensors and Actuators B: Chemical

107

View full text Add to dashboard Cite

A kind of biocompatible fluorescent nitrogen doped carbon quantum dots (NCDs) has been synthesized using uric acid as raw material through a facile, effective and green thermal treatment method in one pot. The surface morphology and optical properties of the synthesized NCDs were characterized. Without further chemical modification, the NCDs emit bright blue fluorescence with a high quantum yield of 56% due to the enhancement effect of N dopant atoms on a surface of carbon quantum dots. Owing to the strong blue fluorescence, negligible toxicity and the special response of heavy metal ions, the NCDs have been used as a fluorescence probe for sensitive and selective detection of Hg 2+ and Ag + ions with detection limit of 4.8 nM and 1 nM, respectively. Moreover, it was successfully applied to determination of Hg 2+ and Ag + in real environmental water samples, which offers the advantages of simplicity and cost efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution

Ren

Zhang

et al. 2017

Sensors and Actuators B: Chemical

107

View full text Add to dashboard Cite

show abstract

“…It can be seen that for Hg 2+ and Ag + concentrations ranging from 0 to 1600 nM, the target concentration and the fluorescence change show a good correlation ( Figure 6 and Figure 7 ). The equation of linear regression for Hg 2+ was y = −905.79x + 8190 ( R 2 = 0.9915), the limit of detection (LOD) based on the

calculation (

is the standard deviation for the blank solution, and S is the slope of the calibration curve) was 3.9 pM [ 3 , 49 , 50 ]. Similarly, the equation of linear regression for Ag + was y = −914.86x + 8455 ( R 2 = 0.9903) and the limit of detection was 3.9 pM.…”

Section: Resultsmentioning

confidence: 99%

A Multifunctional Molecular Probe for Detecting Hg2+ and Ag+ Based on Ion-Mediated Base Mismatch

Wang

Zhang

Dong

2018

Sensors

View full text Add to dashboard Cite

In this paper, a multifunctional biosensing platform for sensitively detecting Hg2+ and Ag+, based on ion-mediated base mismatch, fluorescent labeling, and strand displacement, is introduced. The sensor can also be used as an OR logic gate, the multifunctional design of sensors is realized. Firstly, orthogonal experiments with three factors and three levels were carried out on the designed sensor, and preliminary optimization of conditions was performed for subsequent experiments. Next, the designed sensor was tested the specificity and target selectivity under the optimized conditions, and the application to actual environmental samples further verified the feasibility. Generally, this is a convenient, fast, stable, and low-cost method that provides a variety of ideas and an experimental basis for subsequent research.

show abstract

“…Up to now, several papers have reported the effects of QDs on proteins, DNA, cells, and so forth. [2][3][4][5][6][7], and obvious biological toxicity of QDs has been detected and confirmed. For example, Shen et al have studied the interaction between human hemoglobin and CdS QDs by spectroscopic analysis and confirmed that both electrostatic forces and chemical bonds play an important role in the interaction [6].…”

Section: Introductionmentioning

confidence: 81%

“…where is the gas constant, 8.314 J mol −1 K −1 ; is the temperature (K); is the equilibrium constant at the corresponding temperature, which stands for the static quenching constant ( SV ) in the present paper [10]. According to (3) and 4for plotting the ln ∼ (1/ ) diagram [33], the value of Δ was calculated, and then Δ and Δ were estimated based on the slope of the curve (Table 2).…”

Section: Svmentioning

confidence: 99%

Probing the Effect of Ag₂S Quantum Dots on Human Serum Albumin Using Spectral Techniques

Guan

Liang

et al. 2017

Journal of Nanomaterials

View full text Add to dashboard Cite

The understanding of the interaction between protein and quantum dots (QDs) has significant implications for biological applications of QDs. Herein, we studied the effect of Ag2S QDs on human serum albumin (HSA) using UV-Vis absorption spectra and fluorescence spectroscopy and found that the fluorescence intensity of HSA was gradually decreased with increasing Ag2S QDs concentrations. By using the Stern-Volmer equation for the fluorescence quenching constant (KSV) of the response of Ag2S QDs to HSA as well as thermodynamic equations, the values of thermodynamic enthalpy change (ΔHθ), entropy change (ΔSθ), and free energy change (ΔGθ) were calculated to be −10.79 KJ·mol−1, 37.80 J·mol−1·K−1, and −22.27 KJ·mol−1, respectively. The results indicate that Ag2S QDs exert an obvious static fluorescence quenching effect on HSA and electrostatic interaction plays a key role in the binding process. Furthermore, Raman spectral analysis reveals that Ag2S QDs alter the external environment of tyrosine and tryptophan or the C-H bending of HSA but not the α-helical content.

show abstract

DNA-stabilized silver nanoclusters and carbon nanoparticles oxide: A sensitive platform for label-free fluorescence turn-on detection of HIV-DNA sequences

Cited by 86 publications

References 53 publications

One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution

One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution

A Multifunctional Molecular Probe for Detecting Hg2+ and Ag+ Based on Ion-Mediated Base Mismatch

Probing the Effect of Ag₂S Quantum Dots on Human Serum Albumin Using Spectral Techniques

Contact Info

Product

Resources

About

DNA-stabilized silver nanoclusters and carbon nanoparticles oxide: A sensitive platform for label-free fluorescence turn-on detection of HIV-DNA sequences

Cited by 86 publications

References 53 publications

One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution

One pot synthesis of highly fluorescent N doped C-dots and used as fluorescent probe detection for Hg2+ and Ag+ in aqueous solution

A Multifunctional Molecular Probe for Detecting Hg2+ and Ag+ Based on Ion-Mediated Base Mismatch

Probing the Effect of Ag2S Quantum Dots on Human Serum Albumin Using Spectral Techniques

Contact Info

Product

Resources

About

Probing the Effect of Ag₂S Quantum Dots on Human Serum Albumin Using Spectral Techniques