DNA Sequencing on a Chip

Noble, Deborah

doi:10.1021/ac00101a728

Cited by 14 publications

(7 citation statements)

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“…After probe immobilization and MCU incubation, specificity tests of complementary and non-complementary DNA targets were conducted. The rate of DNA hybridization is dependent on DNA length, with shorter duplex regions showing higher hybridization rates ( Noble, 1995 , Steel et al, 2000 ). Based on the 24-base DNA target used in the experiments, target incubation was set for 30 min at room temperature (23 °C).…”

Section: Resultsmentioning

confidence: 99%

A sensitive DNA capacitive biosensor using interdigitated electrodes

Wang

Veselinović

Yang

et al. 2017

Biosensors and Bioelectronics

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This paper presents a label-free affinity-based capacitive biosensor using interdigitated electrodes. Using an optimized process of DNA probe preparation to minimize the effect of contaminants in commercial thiolated DNA probe, the electrode surface was functionalized with the 24-nucleotide DNA probes based on the West Nile virus sequence (Kunjin strain). The biosensor has the ability to detect complementary DNA fragments with a detection limit down to 20 DNA target molecules (1.5 aM range), making it suitable for a practical point-of-care (POC) platform for low target count clinical applications without the need for amplification. The reproducibility of the biosensor detection was improved with efficient covalent immobilization of purified single-stranded DNA probe oligomers on cleaned gold microelectrodes. In addition to the low detection limit, the biosensor showed a dynamic range of detection from 1 μL−1 to 105 μL−1 target molecules (20 to 2 million targets), making it suitable for sample analysis in a typical clinical application environment. The binding results presented in this paper were validated using fluorescent oligomers.

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Section: Resultsmentioning

confidence: 99%

A sensitive DNA capacitive biosensor using interdigitated electrodes

Wang

Veselinović

Yang

et al. 2017

Biosensors and Bioelectronics

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“…Successful accomplishment of this task may lead to a rapid diagnosis of these diseases. Preliminary experiments were performed also towards another important future direction, the development of DNA chips [9]. For this purpose, we explored the coupling of the PSA operation with microfabricated thick film sensors.…”

Section: Resultsmentioning

confidence: 99%

Adsorptive stripping potentiometry of DNA at electrochemically pretreated carbon paste electrodes

et al. 1996

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Potentiometric stripping analysis is employed for highly sensitive measurements of single-stranded DNA at carbon paste electrodes. Electrochemical (oxidative) pretreatment is used to condition the carbon paste surface prior to the accumulation process. The low background response of the potentiometric stripping mode offers convenient quantitation of low nanogram quantities of DNA. Experimental variables of the pretreatment and measurement procedures are explored and optimized. Results are also reported for the quantitation of nanogram levels of the long terminal repeat (LTR) from the human leukemia virus and for the stripping operation of microfabricated screen-printed electrodes.

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“…To selectively detect a unique human DNA sequence it has been reported that the DNA probe must have at least 16 bases [24]. As the hybridization speed is dependent on the probe length, with a higher speed for shorter probes, an overlapping array of short (6 ± 20 bases) oligonucleotide strands on a silicon chip can be used for evaluation of the sequence of a DNA sample [25].…”

Section: Hybridization Studiesmentioning

confidence: 99%

A Feasibility Study of a Capacitive Biosensor for Direct Detection of DNA Hybridization

Berggren¹,

Stålhandske²,

Brundell³

et al. 1999

Electroanalysis

216

127

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This preliminary study was performed to prove the feasibility of a direct capacitive DNA biosensor for detection of nucleic acids. Two different methods for immobilization of the oligonucleotide probes were used. The ®rst type of sensor was composed of a gold rod with a self-assembled monolayer of a 26-base long oligonucleotide probe, modi®ed with an SH-group at the 5 H -end. Coverage studies showed that only around 20% of the surface was covered, probably due to the bulky nature of the probes. Hybridization studies performed in a¯ow-through cell showed selectivity towards a DNA sample containing single stranded fragments of cytomegalo virus (CMV) possessing a complementary sequence. As few as 25 molecules could be detected at sample concentrations of 0.2 attomolar with an injection volume of 250 mL. Controls with fragments of double-stranded CMV and single-stranded hepatitis B virus and tyrosinase mRNA gave all lower responses. The other type of sensor was modi®ed by covalent immobilization of a phosphorylated 8-base long oligonucleotide probe to a self-assembled monolayer of cysteamine. This biosensor also showed selectivity against single stranded fragments of CMV and also in this case as few as 25 molecules could be detected.

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DNA Sequencing on a Chip

Cited by 14 publications

References 0 publications

A sensitive DNA capacitive biosensor using interdigitated electrodes

A sensitive DNA capacitive biosensor using interdigitated electrodes

Adsorptive stripping potentiometry of DNA at electrochemically pretreated carbon paste electrodes

A Feasibility Study of a Capacitive Biosensor for Direct Detection of DNA Hybridization

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