A sensitive DNA capacitive biosensor using interdigitated electrodes

Wang, Lei; Veselinović, Milena; Yang, Liu; Geiss, Brian J.; Dandy, David S.; Chen, Tom

doi:10.1016/j.bios.2016.09.006

Cited by 95 publications

(72 citation statements)

References 48 publications

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“…The electrodes were created using standard photolithographic microfabrication techniques to create 2 μm of spacing between electrode digits that are 4 μm wide. Many other groups have created IDEs, but they typically use bigger dimensions as these are easier to microfabricate [ 24 , 25 , 26 , 27 ]. Some other groups have developed structures that are more complex to enhance sensitivity [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Using Impedance Measurements to Characterize Surface Modified with Gold Nanoparticles

MacKay

Abdelrasoul

Tamura

et al. 2017

Sensors

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With the increased practice of preventative healthcare to help reduce costs worldwide, sensor technology improvement is vital to patient care. Point-of-care (POC) diagnostics can reduce time and lower labor in testing, and can effectively avoid transporting costs because of portable designs. Label-free detection allows for greater versatility in the detection of biological molecules. Here, we describe the use of an impedance-based POC biosensor that can detect changes in the surface modification of a micro-fabricated chip using impedance spectroscopy. Gold nanoparticles (GNPs) have been employed to evaluate the sensing ability of our new chip using impedance measurements. Furthermore, we used impedance measurements to monitor surface functionalization progress on the sensor’s interdigitated electrodes (IDEs). Electrodes made from aluminum and gold were employed and the results were analyzed to compare the impact of electrode material. GNPs coated with mercaptoundecanoic acid were also used as a model of biomolecules to greatly enhance chemical affinity to the silicon substrate. The portable sensor can be used as an alternative technology to ELISA (enzyme-linked immunosorbent assays) and polymerase chain reaction (PCR)-based techniques. This system has advantages over PCR and ELISA both in the amount of time required for testing and the ease of use of our sensor. With other techniques, larger, expensive equipment must be utilized in a lab environment, and procedures have to be carried out by trained professionals. The simplicity of our sensor system can lead to an automated and portable sensing system.

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Section: Introductionmentioning

confidence: 99%

Using Impedance Measurements to Characterize Surface Modified with Gold Nanoparticles

MacKay

Abdelrasoul

Tamura

et al. 2017

Sensors

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“…Specifically, for the detection of nucleic acids, studies have demonstrated the successful fabrication of microelectode devices using photolithographic methods and the development of nucleic acid sensors which use EIS as the measurement technique. Examples include the detection of BRCA1 gene in breast cancer using interdigitated electrodes 15 the measurement of matched an mismatched DNA for detection of mutations 16 the measurement of 16SrRNA for antibiotic susceptibility testing 17 and the detection of West Nile virus using interdigitated microelectrodes 18 .…”

Section: Introductionmentioning

confidence: 99%

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Piper

Schmueser

et al. 2017

Analyst

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Due to their electroanalytical advantages, microelectrodes are a very attractive technology for sensing and monitoring applications. One highly important application is measurement of DNA hybridisation to detect a wide range of clinically important phenomena, including single nucleotide polymorphisms (SNPs), mutations and drug resistance genes. The use of electrochemical impedance spectroscopy (EIS) for measurement of DNA hybridisation is well established for large electrodes but as yet remains relatively unexplored for microelectrodes due to difficulties associated with electrode functionalisation and impedimetric response interpretation. To shed light on this, microelectrodes were initially fabricated using photolithography and characterised electrochemically to ensure their responses matched established theory. Electrodes with different radii (50, 25, 15 and 5 µm) were then functionalised with a mixed film of 6-mercapto-1-hexanol and a thiolated single stranded ssDNA capture probe for a specific gene from the antibiotic resistant bacterium MRSA. The complementary oligonucleotide target from the mecA MRSA gene was hybridised with the surface tethered ssDNA probe. The EIS response was evaluated as a function of electrode radius and it was found that charge-transfer (R CT ) was more significantly affected by hybridisation of the mecA gene than the non-linear resistance (R NL ) which is associated with the steady state current. The discrimination of mecA hybridisation improved as electrode radius reduced with the R CT component of the response becoming increasingly dominant for smaller radii. It was possible to utilise these findings to produce a real time measurement of oligonucleotide binding where changes in R CT were evident one minute after nanomolar target addition. These data provide a systematic account of the effect of microelectrode radius on the measurement of hybridisation, providing insight into critical aspects of sensor design and implementation for the measurement of clinically important DNA sequences. The findings open up the possibility of developing rapid, sensitive DNA based measurements using microelectrodes.

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“…It has been established for gold surfaces that Faradaic and non-Faradaic EIS measurements can yield approximately equal sensitivity and so it is possible to dispense with a redox couple all together. In fact non-Faradaic EIS measurements have shown the CDL can be used with interdigitated gold electrodes to achieve attomolar sensitivity levels for DNA detection and that voltage pulses and the resulting non-Faradaic currents can be used to measure DNA hybridization in the pM to nM range (Fernandes et al, 2014;Hsu et al, 2016;Wang et al, 2017). Owing to the sensitivity of EIS, the placement of the reference electrode can affect the response (Dimaki et al, 2014).…”

Section: Eismentioning

confidence: 99%

A review of microfabricated electrochemical biosensors for DNA detection

Blair

Corrigan

2019

Biosensors and Bioelectronics

126

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This review article presents an overview of recent work on electrochemical biosensors developed using microfabrication processes, particularly sensors used to achieve sensitive and specific detection of DNA sequences. Such devices are important as they lend themselves to miniaturisation, reproducible mass-manufacture, and integration with other previously existing technologies and production methods. The review describes the current state of these biosensors, novel methods used to produce them or enhance their sensing properties, and pathways to deployment of a complete point-ofcare biosensing system in a clinical setting.

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A sensitive DNA capacitive biosensor using interdigitated electrodes

Cited by 95 publications

References 48 publications

Using Impedance Measurements to Characterize Surface Modified with Gold Nanoparticles

Using Impedance Measurements to Characterize Surface Modified with Gold Nanoparticles

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

A review of microfabricated electrochemical biosensors for DNA detection

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