DNA sequence from butterfly frass and exuviae

Feinstein, Julie

doi:10.1023/b:coge.0000014058.34840.94

Cited by 34 publications

(26 citation statements)

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“…Additionally, the combined protocol was also successfully applied in the extraction of DNA from cicada exuviae ( Figure 1E). Feinstein (2004) and Su et al (2007) have emphasized that DNA extraction protocol to insect exuviae are important to perform population analyses once do not need collect living wild animal. In fact, the combined use of these protocols increases the possibility of obtaining high quality DNA from diverse biological materials by using safe and inexpensive reagents.…”

Section: Resultsmentioning

confidence: 99%

Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Oliveira¹,

Wallau²,

Loreto³

2009

Electron. J. Biotechnol.

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High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first protocol utilizes the enzyme chymosin, normally used as "rennet" in cheese production and which is easily obtained on the commercial market. The second protocol uses "rennet DNA extraction protocol" combined with the DNA binding capacity of glass powder (glass milk), which can easily be "home made". The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples.

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Section: Resultsmentioning

confidence: 99%

Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Oliveira¹,

Wallau²,

Loreto³

2009

Electron. J. Biotechnol.

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“…The use of DNA extracted from faecal material is a well‐developed approach in non‐destructive molecular analysis, especially in mammals and birds . However, there is limited literature on the application of molecular analysis using DNA extracted from faecal samples in insects . In the present study, the possibility of developing a diagnostic test to confirm the presence of A. chinensis from environmental frass samples was investigated.…”

Section: Introductionmentioning

confidence: 99%

Managing outbreaks of the citrus long‐horned beetle Anoplophora chinensis (Forster) in Europe: molecular diagnosis of plant infestation

Strangi¹,

Peverieri²,

Roversi³

2012

Pest Management Science

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“…One noninvasive technique involves collection of frass (excreted insect waste) or exuviae (remains of the exoskeleton). This methodology is most useful in organisms that have at least one stage of their life cycle that produces a large quantity of frass, such as caterpillars, or when individuals are likely to frequently shed their exoskeleton (Feinstein 2004; Watts et al. 2005).…”

Section: Introductionmentioning

confidence: 99%

“…One noninvasive technique involves collection of frass (excreted insect waste) or exu-viae (remains of the exoskeleton). This methodology is most useful in organisms that have at least one stage of their life cycle that produces a large quantity of frass, such as caterpillars, or when individuals are likely to frequently shed their exoskeleton (Feinstein 2004;Watts et al 2005). These collection methods, however, require large amounts of time to observe moulting or involve isolating individuals from others in the population and from their natural environment (Monroe et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Nondestructive sampling of insect DNA from defensive secretion

Donald

Wood

Benowitz

et al. 2012

Molecular Ecology Resources

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Nondestructive techniques to obtain DNA from organisms can further genetic analyses such as estimating genetic diversity, dispersal and lifetime fitness, without permanently removing individuals from the population or removing body parts. Possible DNA sources for insects include frass, exuviae, and wing and leg clippings. However, these are not feasible approaches for organisms that cannot be removed from their natural environment for long periods or when adverse effects of tissue removal must be avoided. This study evaluated the impacts and efficacy of extracting haemolymph from a defensive secretion to obtain DNA for amplification of microsatellites using a nondestructive technique. A secretion containing haemolymph was obtained from Bolitotherus cornutus (the forked fungus beetle) by perturbation of the defensive gland with a capillary tube. A laboratory experiment demonstrated that the sampling methodology had no impact on mortality, reproductive success or gland expression. To evaluate the quality of DNA obtained in natural samples, haemolymph was collected from 187 individuals in the field and successfully genotyped at nine microsatellite loci for 95.7% of samples. These results indicate that haemolymph-rich defensive secretions contain DNA and can be sampled without negative impacts on the health or fitness of individual insects.

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DNA sequence from butterfly frass and exuviae

Cited by 34 publications

References 1 publication

Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Managing outbreaks of the citrus long‐horned beetle Anoplophora chinensis (Forster) in Europe: molecular diagnosis of plant infestation

Nondestructive sampling of insect DNA from defensive secretion

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