DNA replication pattern and cell wall growth in Escherichia coli PAT 84

Koppes, L. J. H.; Overbeeke, Nico; Nanninga, N.

doi:10.1128/jb.133.3.1053-1061.1978

Cited by 43 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various attempts have been made to elucidate the topography of Escherichia coli envelope growth. For instance, use has been made of the incorporation of radioactive constituents such as 3H-labeled meso-DL-2,6-diaminopimelic acid (DAP) specific for the murein layer (8,10,13), the location of penicillin (3)or ampicillin-sensitive sites (15), and the location of bacteriophage receptor sites (1,12). So far no general agreement exists concerning the mode of growth, which clearly is a reflection of the complexity of the process of bacterial cell elongation.…”

mentioning

confidence: 99%

Pattern of meso-dl-2,6-diaminopimelic acid incorporation during the division cycle of Escherichia coli

Verwer¹,

Nanninga²

1980

J Bacteriol

Self Cite

View full text Add to dashboard Cite

The topography of meso-DL-2,6-diaminopimelic acid incorporation into the cell envelope of Escherichia coli W7 (doubling time, , = 70 min) has been studied by autoradiography. To follow the incorporation pattern during the division cycle, cells have been classified according to length and the silver grain distributions have been determined in the two cell halves. In particular, the question of equivalence (with respect to the grain distributions) of the two cell halves has been investigated statistically. The grain localizations have been determined separately for the left cell halves (highest number of grains) and right cell halves. The highest probability of finding grains was in the central area for cells of all length classes. In the longest cells (dividing or nondividing) incorporation occurred in the future septal regions of the prospective daughter cells. Autoradiography of tritiated thymidine-labeled cells indicated the presence of an atypical deoxyribonucleic acid replication cycle (at T = 70 min). Initiation of deoxyribonucleic acid replication occurred during the latter part of the division cycle, and its termination occurred in the next cycle.Surface growth in bacteria is a complex phenomenon. In rod-shaped bacteria, which maintain a more or less constant cell width, this includes overall length growth, i.e., how doubling in length is achieved, and also at which sites growth occurs. It is the latter point which is the subject of this paper. Various attempts have been made to elucidate the topography of Escherichia coli envelope growth. For instance, use has been made of the incorporation of radioactive constituents such as 3H-labeled meso-DL-2,6-diaminopimelic acid (DAP) specific for the murein layer (8, 10, 13), the location of penicillin (3)or ampicillin-sensitive sites (15), and the location of bacteriophage receptor sites (1, 12). So far no general agreement exists concerning the mode of growth, which clearly is a reflection of the complexity of the process of bacterial cell elongation.Earlier autoradiographic studies on [3H]DAPpulse-labeled E. coli cells (10, 13) have been carried out on a limited number of cell classes (three). Recently, we made a more detailed study of [3H]DAP incorporation in E. coli PAT 84 (8). In that case we also found a central area of incorporation and an indication for additional lateral zones in the longest cells.In these studies (8, 10, 13) the silver grain location has been measured with respect to either the geometric center (in nondividing cells) or the visible cell constriction. Thus, it has been assumed that the two cell halves in nondividing and in dividing cells are interchangeable. A possible asymmetry in grain location would not have been detected in this way.The work reported here is intended to provide more quantitative information about the silver grain distribution over whole-mount preparations of [3H]DAP-pulse-labeled E. coli W7 cells.In particular, we have studied the question of whether two cell halves are equivalent with respect to location and quantity...

show abstract

mentioning

confidence: 99%

Pattern of meso-dl-2,6-diaminopimelic acid incorporation during the division cycle of Escherichia coli

Verwer¹,

Nanninga²

1980

J Bacteriol

Self Cite

View full text Add to dashboard Cite

show abstract

“…As observed in synchronized cultures, the activity of peptidoglycan hydrolysis seems to increase sharply just before cell division [130]. The activity of peptidoglycan synthesis increases either at a critical cell length [22], or at a critical age [131,132]. Other authors have observed that one of the precursors, diaminopimelic acid, is incorporated into the peptidoglycan at a rate that increases regularly until it reaches a maximum just before septation initiation.…”

Section: Peptidoglycan Metabolismmentioning

confidence: 84%

“…For instance, auxotrophic strains, starved for an amino acid, initiate their DNA replication soon after readdition of the missing amino acid, at a cell mass 20-30% lower (cell diameter decreases by about 20% [49]) than the normal critical initiation mass (i.e., without starvation) [50][51][52]. Along the same lines, two substrains of E. coil B/r have different critical initiation masses [22]. Finally, the notion of initiator protein arose from the fact that, in the absence of protein synthesis, one did not observe any re-initiation [53].…”

Section: Models Bearing On the Control Of Dna Initiationmentioning

confidence: 99%

The cell cycle ofEscherichia coliand some of its regulatory systems

Képès¹

1986

FEMS Microbiology Letters

View full text Add to dashboard Cite

“…Previous studies have shown that in E. coli both the peptidoglycan layer (Hoffman et al, 1972;Koppes et al, 1978) and the proteins in the outer membrane (Churchward & Holland, 1976) are laid down linearly during the cell cycle; the rate of synthesis of these components is constant in the early part of the cell cycle but doubles abruptly a short time before division. In contrast, the rate of synthesis of the great majority of cellular proteins, including those in the inner membrane which constitute approximately 50 YO of all envelope protein, increases exponentially throughout the cell cycle.…”

Section: Discussionmentioning

confidence: 99%

Envelope Protein Synthesis and Inhibition of Cell Division in Escherichia coli during Inactivation of the B Subunit of DNA Gyrase

Herrero¹,

Nf²,

Ib³

1982

Microbiology

View full text Add to dashboard Cite

The rates of synthesis of inner and outer membrane proteins of Escherichia coli K12 during inhibition of cell division have been studied. When cell division was inhibited, either by treatment of wild-type cells with the antibiotic clorobiocin (an inhibitor of the B subunit of DNA gyrase) or by a temperature shift of a gyrB-ts mutant, a 40% reduction in the rate of synthesis of total outer membrane protein relative to that of the inner membrane was observed. When a gyrB-ts mutant was shifted to high temperature under conditions which allowed continued cell division, this large reduction in the rate of synthesis of outer membrane protein relative to inner membrane protein was not observed. In contrast to the results obtained with clorobiocin, inhibition of cell division by the beta-lactam antibiotic cefuroxime did not cause any detectable disturbance in the rate of synthesis of either inner or outer membrane protein. This demonstrates that inhibition of septum formation per se does not perturb synthesis of envelope protein. The data obtained are consistent with a model in which the rate of synthesis and therefore expansion of outer membrane is one of many conditions which must be satisfied before septum formation can occur. The results are discussed in relation to such a model, and to previous findings which have shown that the rate of synthesis of outer membrane proteins displays a linear mode with an abrupt doubling in rate at a discrete point in the cell cycle.

show abstract

DNA replication pattern and cell wall growth in Escherichia coli PAT 84

Abstract: An electron microscopic radioautographic study was made of tritiated thymidine incorporation into the genome of Escherichia coli PAT 84 and of tritiated

Cited by 43 publications

References 23 publications

Pattern of meso-dl-2,6-diaminopimelic acid incorporation during the division cycle of Escherichia coli

Pattern of meso-dl-2,6-diaminopimelic acid incorporation during the division cycle of Escherichia coli

The cell cycle ofEscherichia coliand some of its regulatory systems

Envelope Protein Synthesis and Inhibition of Cell Division in Escherichia coli during Inactivation of the B Subunit of DNA Gyrase

Contact Info

Product

Resources

About