DNA repair test of disinfectants by liquid rec-assay

Sakagami, Yoshikazu; Yamasaki, Katsuhiro; Yokoyama, H.; Ose, Youki; Sato, Takahiko

doi:10.1016/0167-8817(88)90004-1

Cited by 17 publications

(9 citation statements)

References 10 publications

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“…Both positive and negative results have been reported in in vitro mutagenicity studies [76][77][78][79][80][81][82][83][84][85][86] , while almost all in vivo tests have yielded negative results 75,76,87,88) . GA exhibited mild to strong mutagenic effects with and without S9 metabolic activation in S. typhimurium strain TA102 [77][78][79] .…”

Section: Genotoxicity and Mutagenicitymentioning

confidence: 98%

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Effects of Glutaraldehyde Exposure on Human Health

Takigawa

Endo

2006

Journal of Occupational Health

243

147

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Section: Genotoxicity and Mutagenicitymentioning

confidence: 98%

“…GA was not mutagenic with or without S9 in TA98, TA1535, TA1537 and TA1538 76,81) . GA was positive in the DNA repair test by liquid recassay 83) and by umu test without S9 activation 84) . GA was mutagenic in E. coli WP2 tester strains 79) , but yielded negative results in the SOS chromotest with E. coli PQ37 80) .…”

Section: Genotoxicity and Mutagenicitymentioning

confidence: 99%

Effects of Glutaraldehyde Exposure on Human Health

Takigawa

Endo

2006

Journal of Occupational Health

243

147

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“…In the presence of metabolic activation there was a maximum of a 1.9-2.3-fold increase in revertants at non-toxic dosages. Previous studies with guanine-cytosine revertants also have not demonstrated mutagenic activity with TA98, TA1535, TA1537 and TA1538 but have demonstrated weak (only around twofold increases) increases with TA100 (Haworth et al, 1983;Slesinski et al, 1983;Sakagami et al, 1988a). Other studies with TA102, which has an adenine-thymine (A-T) base pair within the hisG gene (Levin et al, 1982) and has been shown to be more sensitive than guanine-cytosine base pairs (Ruiz-Rubio et al, 1985), has produced dosage-related positive results with GA (Levin et al, 1982;Wilcox et al, 1990;Jung et al, 1992;Watanabe et al, 1998).…”

Section: Discussionmentioning

confidence: 81%

Genetic toxicology studies with glutaraldehyde

Vergnes¹,

Ballantyne

2002

J of Applied Toxicology

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Glutaraldehyde (GA; CAS no. 111-30-8) has a wide spectrum of industrial, scientific and biomedical applications, with a potential for human exposure particularly in its biocidal applications. The likelihood for genotoxic effects was investigated in vitro and in vivo. A Salmonella typhimurium reverse mutation assay showed no evidence for mutagenic activity with strains TA98, TA1535, TA1537 and TA1538, with or without metabolic activation. However, there was a weak mutagenic response (1.9-2.3-fold at the highest non-toxic concentration) with TA100 in the presence of metabolic activation. In a Chinese hamster ovary (CHO) forward gene mutation assay (HGPRT locus) there were no consistent, statistically significant, reproducible or dosage-related increases in the frequency of 6-thioguanine resistant cells. There were no reproducible or dosage-related increases in sister chromatid exchanges in an in vitro test in CHO cells. An in vitro cytogenetics study in CHO cells showed no evidence for an increase in chromosomal aberrations on treatment with GA, either in the presence or absence of metabolic activation. In vivo, a mouse peripheral blood micronucleus test showed no increase in micronucleated polychromatophils at sampling times of 30, 48 and 72 h after acute gavage dosing with GA at 40, 80 and 125 mg kg(-1) (corresponding to 25, 50 and 85% of the LD(50)). The absence of an in vivo clastogenic potential was confirmed by no increase in chromosomal aberrations in a rat bone marrow cytogenetics study with sampling at 12, 24 and 48 h after acute gavage dosing with GA (12.5, 30 or 60 mg kg(-1) with males, and 7.5, 20 or 40 mg kg(-1) with females). Thus, in this series of tests, GA produced genotoxic effects in vitro only in a bacterial reverse mutation assay with no evidence for in vivo genotoxicity.

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“…Then 0.1 ml of this mixture was poured into the second one. This procedure was repeated 10 times to prepare serial halfdilutions of the compounds (Sakagami et al, 1988;Sharma and Sobti, 2000). Thereafter, the plates were inoculated with a 40-ml portion of spore solutions of rec + and rec -(1.3 · 10 8 ), covered with lids, and incubated overnight at 378C.…”

Section: Preparation Of Sporesmentioning

confidence: 99%

A study on the genotoxic activities of some new benzoxazoles

et al. 2007

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The Bacillus subtilis rec assay has been specially developed to detect DNA-damaging potential in chemicals, with the rationale based on the relative difference of survival of a DNA repair combination proficient strains and its deficient strain, which is interpreted as genotoxicity. The genotoxic activities of newly (1-6) and previously (7-18) synthesized various benzoxazoles and benzimidazoles were analyzed via the B. subtilis rec assay. Newly obtained benzoxazole and benzimidazole derivatives (1-6) were synthesized in the presence of polyphosphoric acid (PPA) and 6 N HCl, respectively to detect their DNA-damaging activities. Among the tested compounds, 6-methyl-2-(o-chlorophenyl)benzoxazole (9), 5-amino-2-(p-methylbenzyl)benzoxazole (4), 5-(p-fluorobenzamido)-2-phenylbenzoxazole (13), and 2-(p-methylaminophenyl)benzoxazole (18) showed genotoxic activities having Rec 50 values of 1.85, 1.74, 1.60, and 1.50 or S-probit values of 0.534, 0.482, 0.460, and 0.357, respectively. On the other hand, 2-(p-bromobenzyl)-5-methylbenzimidazole (6) and 2-benzyl-5-(p-fluorophenylacetamido)-benzoxazole (15) were exhibited a reverse effect that displayed a bacterial growth in the recstrains while there was no any bacterial growth in rec + strains at the same concentration.

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DNA repair test of disinfectants by liquid rec-assay

Cited by 17 publications

References 10 publications

Effects of Glutaraldehyde Exposure on Human Health

Effects of Glutaraldehyde Exposure on Human Health

Genetic toxicology studies with glutaraldehyde

A study on the genotoxic activities of some new benzoxazoles

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