Dna Relatedness Among Wild and Domesticated Species in the<i>Aspergillus Flavus</i>Group

Kurtzman, Cletus P.; Smiley, M. J.; Robnett, Christie J.; Wicklow, Donald T.

doi:10.1080/00275514.1986.12025355

Cited by 84 publications

(29 citation statements)

References 17 publications

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“…This indicated that A. oryzae and A. sojae were very closely related species to A. favus and A. parasiticus, respectively, consistent with the DNA complementarity of Kurtzman et al (7). DISCUSSION We found that the restriction enzyme, SmaI, produced the restriction cleavage patterns that allow the closely related species of koji molds, A, oryzae, A. sojae, A. flavus and A, parasiticus to be distinguished.…”

Section: Resultssupporting

confidence: 66%

Rapid differentiation of four related species of Koji molds by agarose gel electrophoresis of genomic DNA digested with SmaI restriction enzyme.

Gomi

Tanaka

Iimura

et al. 1989

J. Gen. Appl. Microbiol.

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We describe a simple and rapid procedure for differentiating the koji molds, Aspergillus oryzae and A. sojae, from the closely related species, A. flavus and A. parasiticus. Total DNA from the Aspergillus strains was digested with the restriction enzyme SmaI and was separated by electrophoresis on an agarose gel. The size of the bands, ranging from 3 to 5 kb strongly stained with ethidium bromide, was intraspecifically consistent but was interspecifically different. The difference in the size of the bands seemed to be due to the divergence in the nucleotide sequence of nuclear ribosomal DNA. The restriction enzyme cleavage patterns can be readily distinguished on agarose gel, so they will provide a useful tool for identifying koji molds and other related species.Aspergillus oryzae and A. sojae are important fungi as koji mold for manufacture of sake, shoyu (soy sauce) and miso in Japan. These two species are very closely related to As flavus and A. parasiticus which produce the potent carcinogen aflatoxin. Therefore, accurate identification of these species is of great importance. Murakami (8) studied the taxonomy of the four species and differentiated the A. oryzae group including A. oryzae and A, sojae from the A. flavus group including A. flavus and A. parasiticus. However, his method, based on some morphological and physiological characteristics is time-consuming and requires experience for accurate differentiation.Recently molecular biological techniques have been used in fungal taxonomy.

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Section: Resultssupporting

confidence: 66%

Rapid differentiation of four related species of Koji molds by agarose gel electrophoresis of genomic DNA digested with SmaI restriction enzyme.

Gomi

Tanaka

Iimura

et al. 1989

J. Gen. Appl. Microbiol.

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“…Microbiology 153 phylogeny of these fungi (Kurtzman et al, 1986;Klich & Mullaney, 1987), morphological and cultural characteristics are still routinely used for identification because of their simplicity, accessibility and feasibility.…”

Section: T Hedayati and Others 1680mentioning

confidence: 99%

Aspergillus flavus: human pathogen, allergen and mycotoxin producer

et al. 2007

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Aspergillus infections have grown in importance in the last years. However, most of the studies have focused on Aspergillus fumigatus, the most prevalent species in the genus. In certain locales and hospitals, Aspergillus flavus is more common in air than A. fumigatus, for unclear reasons. After A. fumigatus, A. flavus is the second leading cause of invasive aspergillosis and it is the most common cause of superficial infection. Experimental invasive infections in mice show A. flavus to be 100-fold more virulent than A. fumigatus in terms of inoculum required. Particularly common clinical syndromes associated with A. flavus include chronic granulomatous sinusitis, keratitis, cutaneous aspergillosis, wound infections and osteomyelitis following trauma and inoculation. Outbreaks associated with A. flavus appear to be associated with single or closely related strains, in contrast to those associated with A. fumigatus. In addition, A. flavus produces aflatoxins, the most toxic and potent hepatocarcinogenic natural compounds ever characterized. Accurate species identification within Aspergillus flavus complex remains difficult due to overlapping morphological and biochemical characteristics, and much taxonomic and population genetics work is necessary to better understand the species and related species. The flavus complex currently includes 23 species or varieties, including two sexual species, Petromyces alliaceus and P. albertensis. The genome of the highly related Aspergillus oryzae is completed and available; that of A. flavus in the final stages of annotation. Our understanding of A. flavus lags far behind that of A. fumigatus. Studies of the genomics, taxonomy, population genetics, pathogenicity, allergenicity and antifungal susceptibility of A. flavus are all required.

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“…DNA-DNA hybridization is the most important method for revealing species assignments among yeasts (Kurtzman and Fell, 1998). Kurtzman et al (1986) studied the relatedness between strains of A. flavus and three phenotypically similar species in section Flavi by nuclear DNA base composition and the total DNA-DNA hybridization. They have concluded that A. flavus, A. oryzae, A. parasiticus, and A. sojae represent a single species.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, molecular genetic approaches have been extended to filamentous fungi such as Aspergillus and related teleomorphs (Kurtzman et al, 1986;Peterson, 1992;Sugiyama et al, 1991;Tamura et al, 1999). Peterson (1999) studied the D1 and D2 regions of the large subunit ribosomal RNA genes (lsu-rDNA) from 215 named taxa in Aspergillus and tried to establish a species concept of these taxa.…”

mentioning

confidence: 99%

Genetic relatedness among species in Aspergillus section Clavati as measured by electrophoretic comparison of enzymes, DNA base cmposition, and DNA-DNA hybridization.

Tamura

Hamamoto

Cañete‐Gibas

et al. 1999

J. Gen. Appl. Microbiol.

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Dna Relatedness Among Wild and Domesticated Species in theAspergillus FlavusGroup

Cited by 84 publications

References 17 publications

Rapid differentiation of four related species of Koji molds by agarose gel electrophoresis of genomic DNA digested with SmaI restriction enzyme.

Rapid differentiation of four related species of Koji molds by agarose gel electrophoresis of genomic DNA digested with SmaI restriction enzyme.

Aspergillus flavus: human pathogen, allergen and mycotoxin producer

Genetic relatedness among species in Aspergillus section Clavati as measured by electrophoretic comparison of enzymes, DNA base cmposition, and DNA-DNA hybridization.

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