DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO<sub>2</sub>/TiO<sub>2</sub>assembly

Păunescu, Daniela; Mora, Carlos A.; Puddu, Michela; Krumeich, Frank; Grass, Robert N.

doi:10.1039/c4tb01552e

Cited by 21 publications

(24 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The DNA storage system within silica further offers exceptional stability against oxidation (see the reactive oxygen species (ROS) test in the Supporting Information), and by adding an additional titania layer the photoresistance of DNA can be greatly improved. [17] In ancient fossil bone, DNA has the greatest chance of survival if encapsulated within apatite/collagen structures [18] and crystal aggregates, [19] which protect the solid DNA from the environment and humidity-very similar to the encapsulation of DNA within the inorganic silica particles utilized here. Indeed if the decay kinetics presented in Figure 2 for DNA encapsulated in silica are extrapolated to lower temperatures, the data coincides well with the degradation rate of DNA in fossil moa bone derived by Allentoft et al from samples up to 8000 years old.…”

mentioning

confidence: 97%

Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes

et al. 2015

View full text Add to dashboard Cite

Information, such as text printed on paper or images projected onto microfilm, can survive for over 500 years. However, the storage of digital information for time frames exceeding 50 years is challenging. Here we show that digital information can be stored on DNA and recovered without errors for considerably longer time frames. To allow for the perfect recovery of the information, we encapsulate the DNA in an inorganic matrix, and employ error-correcting codes to correct storage-related errors. Specifically, we translated 83 kB of information to 4991 DNA segments, each 158 nucleotides long, which were encapsulated in silica. Accelerated aging experiments were performed to measure DNA decay kinetics, which show that data can be archived on DNA for millennia under a wide range of conditions. The original information could be recovered error free, even after treating the DNA in silica at 70 8C for one week. This is thermally equivalent to storing information on DNA in central Europe for 2000 years.Prehistorical information put down by our ancestors in cave drawings, texts engraved in gold, and medieval texts are some of the strongest links with our past. An example is the Archimedes Palimpsest that originates from the tenth century. This contains the single known copy of "The Methods of Mechanical Theorems", and represents a cornerstone in the development of geometry and modern calculus. The book has survived more than 1000 years and in 1998 was valued at more than two million USD. In view of this valuation of information it may seem surprising that current efforts of guaranteeing longevity of digital information are scarce (e.g. MDisc, Syylex) and the storage half-life of information has dropped drastically since the transition from analog to digital storage systems.[1]Traditional storage technologies such as optical and magnetic devices are not reliable for long-term (> 50 years) data storage.[2] Furthermore, the development of reliable systems requires long-term testing, which is well above the current device-development timelines. DNA is the only datastorage medium for which real long-term data are available from archeology. Most recently, 300 000 year old mitochondrial DNA from bears and humans has been sequenced. [3] DNA has also previously been utilized as a coding language, for applications in forensics, [4] product tagging, [5] and DNA computing.[6] As a consequence, several approaches to store information on DNA have been proposed. [7] However, those approaches are not reliable as they cannot handle errors efficiently and do not suggest how to (physically) store the DNA to maintain its stability over time.To overcome these issues we combined an error-correcting information-encoding scheme tailored to DNA (Scheme 1) with a previously established chemical method for storing DNA in "synthetic fossils". The corresponding experiments show that only by the combination of the two concepts, could digital information be recovered from DNA stored at the Global Seed Vault (at À18 8C) after over 1 milli...

show abstract

mentioning

confidence: 97%

Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes

et al. 2015

View full text Add to dashboard Cite

show abstract

“…One possible solution is protecting the DNA code by nanohybridization with ceramic nanoparticles in order to produce a stable nanosystem. [42][43][44][45][46] These encapsulated DNA nanosystems are suitable for code systems. First, they are stable enough to endure severe environments and can maintain information integrity during storage and circulation.…”

Section: Ceramic Nanohybrids With Dna For Bio Codementioning

confidence: 99%

“…Grass and coworkers have developed synthetic DNA "fossils", mimicking the inherent hermetic diffusion barrier of fossils. 17,19,46) DNA was encapsulated into amorphous silica instead of "natural' amber (polymerized terpenes). Silica is a ceramic material with high melting point as well as exceptional barrier property.…”

Section: Design and Synthesis Of Dna@ceramic Nanohybridsmentioning

confidence: 99%

Info-Convergence Ceramic Nanosystems

Jin¹,

Park²

2019

J. Korean Ceram. Soc

View full text Add to dashboard Cite

We face many fascinating and diverse challenges, the most important among which is to determine how to store a large amount of information with novel approaches. Info-convergence ceramic nanosystems, which combine ceramic materials science and information technology, may provide an attractive alternative. This review considers recent multidisciplinary advances in the development of info-convergence nanosystems based on ceramic materials and discusses various strategies under ceramic-based information systems with a special focus on materials and nanohybridization technologies. Ceramic materials have played diverse roles not only as the generic coding support, but also as the central coding substance. The review highlights the ceramic nanohybrid bio code and ceramic nanoparticle optical code for applications in tracking-and-traceability management, nano-forensics, anticounterfeiting, and even communication, as well as the four steps of encoding, encrypting, decrypting, and decoding for the desired applications. Additionally, associated challenges, potential solutions, and perspectives for future developments in the field are discussed.

show abstract

“…The major downside of DNA as a tracer is its limited environmental stability—the biopolymer is prone to chemical decay in the presence of metal ions, non‐neutral pH, strong microbial activity, elevated temperature, and even sunlight . Halter and Zahn, for example, found that DNA has a half‐life of only roughly 2 days in soil .…”

Section: Introductionmentioning

confidence: 99%

Length‐dependent DNA degradation kinetic model: Decay compensation in DNA tracer concentration measurements

et al. 2018

View full text Add to dashboard Cite

DNA is often used as a tracer in both environmental fluid flow characterization and in material tracking to avoid counterfeiting and ensure transparency in product value chains. The main drawback of DNA as a tracer is its limited stability, making quantitative analysis difficult. Here, we study length-dependent DNA decay at elevated temperatures and under sunlight by quantitative PCR and show that the stability of randomly generated DNA sequences is inversely proportional to the sequence length. By quantifying the remaining DNA length distribution, we present a method to determine the extent of decay and to account for it. We propose a correction factor based on the ratio of measured concentrations of two different length sequences. Multiplying the measured DNA concentration by this length-dependent correction factor enables precise DNA tracer quantification, even if DNA molecules have undergone more than 100-fold degradation.

show abstract

DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO₂/TiO₂assembly

Abstract: The here presented method allows to protect DNA against UV-induced damage by encapsulating it in a core–shell–shell particulate construct.

Cited by 21 publications

References 47 publications

Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes

Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes

Info-Convergence Ceramic Nanosystems

Length‐dependent DNA degradation kinetic model: Decay compensation in DNA tracer concentration measurements

Contact Info

Product

Resources

About

DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO2/TiO2assembly

Abstract: The here presented method allows to protect DNA against UV-induced damage by encapsulating it in a core–shell–shell particulate construct.

Cited by 21 publications

References 47 publications

Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes

Robust Chemical Preservation of Digital Information on DNA in Silica with Error‐Correcting Codes

Info-Convergence Ceramic Nanosystems

Length‐dependent DNA degradation kinetic model: Decay compensation in DNA tracer concentration measurements

Contact Info

Product

Resources

About

DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO₂/TiO₂assembly